Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,860,628 T→A I95F (ATC→TTC)  rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,860,6280TA99.2% 387.8 / NA 120I95F (ATC→TTC) rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (55/64);  minor base C (0/1);  total (55/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

AAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAT  >  CP009273/2860486‑2860773
                                                                                                                                              |                                                                                                                                                 
 cgCGGAAACCACGTCCCGGTTCCAACTTCTCCACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCcgg                                                                                                                                            <  2:289493/148‑1 (MQ=255)
     gAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTTGAACATCcggc                                                                                                                                           >  1:1033440/1‑146 (MQ=255)
       aaCCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCcggcgg                                                                                                                                         <  1:1339177/146‑1 (MQ=255)
       aaCCACGTTCCGGGTCAAACTTCTCGACCGAGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCga                                                                                                                                      >  2:917290/1‑149 (MQ=255)
           aCGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAgg                                                                                                                                  >  2:1695319/1‑149 (MQ=255)
             gTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGCCGCAAGTTACTCTCGAACATCCGGCGGCGagag                                                                                                                                   >  1:248088/1‑146 (MQ=255)
             gTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCg                                                                                                                                >  2:953413/1‑149 (MQ=255)
               tCCGGGTCAAACTACTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGCTTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGc                                                                                                                                 <  1:1099152/146‑1 (MQ=255)
                    gTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATACGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACAt                                                                                                                            <  1:542754/146‑1 (MQ=255)
                    gTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACAtccc                                                                                                                         >  2:116055/1‑147 (MQ=255)
                    gTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACAt                                                                                                                            >  1:903587/1‑146 (MQ=255)
                       aaaCTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGCCAtctc                                                                                                                         >  1:396397/1‑146 (MQ=255)
                       aaaCTTCCACACCGCGCGCATCAGCCCCAGGTTGCCCTCTTCGATCAGTGCCAGCAACGCCAGCCCACGTGTGCCATATCGGCGGGCAATTTTTACCATCAGACGCAAGTTACTCTCGAACCTCCGGCGGCGAGAGGCGACATCTCCAc                                                                                                                      <  2:856586/149‑1 (MQ=255)
                         aCTTCTCTACCGCGCGGATCAGCCCCACGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCa                                                                                                                       <  1:927530/146‑1 (MQ=255)
                          cTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCa                                                                                                                   <  2:761408/149‑1 (MQ=255)
                               ctACCGCGCGGATCAGTCCCAGGTTGCCCACTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACGAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTgcg                                                                                                              >  2:1127000/1‑149 (MQ=255)
                                 aCCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCCGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGcgcg                                                                                                            <  2:1177111/149‑1 (MQ=255)
                                 aCCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTgc                                                                                                               <  1:377768/146‑1 (MQ=255)
                                  ccGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGa                                                                                                           >  2:1549555/1‑149 (MQ=255)
                                    gcgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAATTTACTCTCGAACATCCGGCGGCGAAAGGCGACATCTCCACGCAGTGCGCGAcg                                                                                                         >  2:792974/1‑149 (MQ=255)
                                    gcgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGCCATCTCCACGCAGTGcgcg                                                                                                            >  1:1631302/1‑146 (MQ=255)
                                     cgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACCTCCGGCGGCGGGAGGCGACATCTCCACGCAGTGCGCGa                                                                                                           >  1:716372/1‑146 (MQ=255)
                                        ggATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCCACGCCAGACCACGATTGTCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGAcgc                                                                                                        <  1:1448834/146‑1 (MQ=255)
                                          aTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAACTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACgcgc                                                                                                      >  1:1181573/1‑146 (MQ=255)
                                          aTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAATTTTACCACCAGACGCAAGCTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGAccggc                                                                                                      >  1:22991/1‑142 (MQ=255)
                                          aTCAGCCCCAGGTTGCCCTCTTCGAGAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCaaa                                                                                                   <  2:252735/149‑1 (MQ=255)
                                               ccccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCaaaac                                                                                                 >  1:834733/1‑145 (MQ=255)
                                               ccccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATaaa                                                                                              <  2:1667584/149‑1 (MQ=255)
                                                 ccAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCCGTGCGCGACGCGCaaactaaact                                                                                            >  2:677512/1‑149 (MQ=255)
                                                  cAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATACCGGCGGGCAATTTTTACCACCAGACGCATGTTACTCTCGAACATCCGGCGGCTAGAGGCGACATCTCTTCTCAGTGTGCGACGCGTAAAATaaa                                                                                              <  1:445465/146‑1 (MQ=255)
                                                  cAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATaaa                                                                                              <  1:1451104/146‑1 (MQ=255)
                                                  cAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATaaa                                                                                              <  1:289384/146‑1 (MQ=255)
                                                   aGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCACTTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGTCATCTCCACGCAGTGCGCGACGCGCAAAATAAActtc                                                                                          <  2:1151463/149‑1 (MQ=255)
                                                   aGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActtc                                                                                          <  2:756129/149‑1 (MQ=255)
                                                   aGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActtc                                                                                          <  2:1775237/149‑1 (MQ=255)
                                                      ttGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGACCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActtc                                                                                          <  1:48529/146‑1 (MQ=255)
                                                      ttGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCGCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAActtc                                                                                          <  1:49874/146‑1 (MQ=255)
                                                            tctTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGc                                                                                 >  2:816375/1‑149 (MQ=255)
                                                             ctTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCg                                                                                   <  1:313650/146‑1 (MQ=255)
                                                                cGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGtt                                                                             >  2:1434631/1‑149 (MQ=255)
                                                                cGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGtt                                                                             <  2:1446816/149‑1 (MQ=255)
                                                                 gATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCg                                                                               <  1:1346800/146‑1 (MQ=255)
                                                                 gATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGAGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAACATAAACTTCTTCTTCGGCCg                                                                               >  1:1190553/1‑146 (MQ=255)
                                                                      ggTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgt                                                                       >  2:1441870/1‑149 (MQ=255)
                                                                       gcccaccaACGCCAGCCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtg                                                                      <  2:619118/147‑1 (MQ=255)
                                                                        tCCAGCAACGCCATACCACGATTGACATCACGGCGGGCACTTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtgg                                                                     <  2:117726/149‑1 (MQ=255)
                                                                         ccAGCAACGCCAGCCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                                    <  2:1016598/149‑1 (MQ=255)
                                                                         ccAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGCCACAAATAACACTCCGACAACCTGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATCAACTTCTTCTTCGGCCGTTAACAgtggt                                                                    <  2:953253/149‑1 (MQ=255)
                                                                         ccAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                                    <  2:552441/149‑1 (MQ=255)
                                                                         ccAGCAACGCCACACCCCGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAATCTTCTTCTTCGGCCGTTAACAgtggt                                                                    <  2:1552107/149‑1 (MQ=255)
                                                                          cAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAggg                                                                      >  1:1558562/1‑144 (MQ=255)
                                                                           aGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGGCGCGCAAAATAAACTTCTTCTTCGGCCGTTAACGCTGGTGa                                                                  >  2:213708/1‑149 (MQ=255)
                                                                           aGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCGTCTTCGGCCGTTAACAGTGGTGa                                                                  <  2:958165/149‑1 (MQ=255)
                                                                            gCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                                    <  1:656812/146‑1 (MQ=255)
                                                                            gCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                                    >  1:1660985/1‑146 (MQ=255)
                                                                            gCAACGCCAGACCACGATTCCCATAGCGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAgtggt                                                                    <  1:1051061/146‑1 (MQ=255)
                                                                               aCGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGaa                                                                 <  1:280415/146‑1 (MQ=255)
                                                                                cGCCAGACCACGTTTGCCATAGCGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACGTCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAAt                                                                <  1:1163775/146‑1 (MQ=255)
                                                                                   cAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCaa                                                          >  2:1443181/1‑149 (MQ=255)
                                                                                        cACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCa                                                     <  2:359311/149‑1 (MQ=255)
                                                                                        cACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCCCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCa                                                     <  2:359253/149‑1 (MQ=255)
                                                                                         aCGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCACCAAAAACTTCTTCTTCGGCCGTTCACAGTGGGGAATAAcacaccc                                                       >  1:1288357/1‑140 (MQ=255)
                                                                                           gATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGATACTCTCGAACATACGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACACGCAAAAGAAACTGCTGCTTCGGCCGTTAACAGTGGTGAATAACCAAACTCa                                                     >  1:581776/1‑146 (MQ=255)
                                                                                             ttGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGACGTAAACAGTGGTGAATACCCAAtcctacc                                                   <  1:1384325/146‑6 (MQ=255)
                                                                                               gCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCCAACATCCGGCGGCGAGAGGCGCCCTCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCaa                                                 <  1:1471342/146‑1 (MQ=255)
                                                                                                ccATACCGGCGGGCAATTTTTACCACCCGCCGCAAGTTACGCTCGAACATCCGGCGGCGATAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAg                                                <  1:1539795/146‑1 (MQ=255)
                                                                                                ccAGAACCGCGTGCAATTTTTACCACCAGACCCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAg                                                <  1:1486890/146‑1 (MQ=255)
                                                                                                 cATAACGGCTGGCAATTTTTACCACCAGACGCAAGTTACTCACGAACACCCGCCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAgg                                               <  1:583277/146‑1 (MQ=255)
                                                                                                 cATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACACTCGAACATACGGCGGAGAGAGGCGACATCTCCAAGCAGTGCGCGACGCGCAAAATAAACTTCTTATTCGGCCGGTAACAGTGGTGAATAACCAATATCACCaacg                                               >  1:770261/1‑144 (MQ=255)
                                                                                                 cATAACGGCGGGCAATTTTTACCACCAGAAGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCGTCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTaa                                            >  2:1251450/1‑149 (MQ=255)
                                                                                                  aTAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGATGCGACATCTCCACGCAGTGCGCGATGCGCAAAATAAACTTCTTCTTCGGCGGTTAACAGTGGTGAATAACCAATCTCACCAAGGTaaa                                           <  2:1153335/149‑1 (MQ=255)
                                                                                                  aTAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTaaa                                           <  2:1690482/149‑1 (MQ=255)
                                                                                                   tAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGACTAACCAATCTCACCAAGGTAAAg                                          >  2:925716/1‑149 (MQ=255)
                                                                                                     aCGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTaaa                                           <  1:1023403/146‑1 (MQ=255)
                                                                                                      cggcggGCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTATTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTg                                       >  2:608347/1‑149 (MQ=255)
                                                                                                            gCAATTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTcgc                                 >  2:53669/1‑149 (MQ=255)
                                                                                                              aaTTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGCCGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACAAAGGTAAAGCTGCGTcg                                  >  1:619211/1‑146 (MQ=255)
                                                                                                               aTTTTTACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTc                              <  2:1553270/149‑1 (MQ=255)
                                                                                                                tttttACCACCAGCCGCCAGGTACTCTCGAACATCCGGCGCGGGGTGGCGACCTATACTCCCAGTTCACGACGCGCAAGATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTcgcg                                >  1:1160797/1‑146 (MQ=255)
                                                                                                                tttttACCACCAGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGCGTCGCGTcc                             >  2:1049823/1‑149 (MQ=255)
                                                                                                                      ccccccgACGCAAGTTACTCTCGAACCTCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacaca                       <  2:705981/143‑1 (MQ=255)
                                                                                                                      ccaccaGCCGCCAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacaca                       <  2:268475/149‑1 (MQ=255)
                                                                                                                      ccaccaGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTTCAac                          >  1:977711/1‑146 (MQ=255)
                                                                                                                        accaGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg                     >  2:1484731/1‑149 (MQ=255)
                                                                                                                         ccaGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacaca                       <  1:1338487/146‑1 (MQ=255)
                                                                                                                         ccaGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacaca                       >  1:106514/1‑146 (MQ=255)
                                                                                                                           aGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg                     >  1:1034571/1‑146 (MQ=255)
                                                                                                                           aGACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtg                  >  2:587110/1‑149 (MQ=255)
                                                                                                                            gccGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgt                 <  2:1691721/147‑1 (MQ=255)
                                                                                                                            gACGCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgt                 <  2:1691124/149‑1 (MQ=255)
                                                                                                                              cGCAAGTTACTCTCGAACAGCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGGGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtgt               <  2:1480044/149‑1 (MQ=255)
                                                                                                                               gCAAGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAATCGCATCCAACACACGCtgtgtg              >  2:94953/1‑149 (MQ=255)
                                                                                                                                cAAGTTACTCTCGAACCTCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTgg             <  2:1278989/149‑1 (MQ=255)
                                                                                                                                  aGTTACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt           >  2:728380/1‑149 (MQ=255)
                                                                                                                                     tACTCTCGAACATCCGGCGGCGAGAGGCGACATCTCCACGAAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt           <  1:744448/146‑1 (MQ=255)
                                                                                                                                       ctctCGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGAGAATAACCAATCTCACCAAGGTAAAACGGAGTGGCGTCCAACACACGCTGTGTGGCTCCCTg      >  2:1206680/1‑149 (MQ=255)
                                                                                                                                        tctcGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTGCTTCGGCCGTTAACAGTGGTGAATAACCAATCGCACCCAGTGAAAGCTGAGTCGCGTCCAACACACCCTGTGTGGCTccc        >  1:1271460/1‑146 (MQ=255)
                                                                                                                                          tcGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAAGACACGCTGTGTGGCTCCCTg      >  1:737894/1‑146 (MQ=255)
                                                                                                                                          tcGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa   <  2:448220/149‑1 (MQ=255)
                                                                                                                                           cGAACATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGAAGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACACCTCACACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGGGAt  >  2:413442/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
AAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAT  >  CP009273/2860486‑2860773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑