Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,176,709 Δ1 bp 6.3% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,176,7090A. 6.3% 182.6 / 7.6 63intergenic (+27/+463)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (36/22);  new base . (2/2);  total (39/24)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

ATGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAA  >  CP009273/3176561‑3176857
                                                                                                                                                     |                                                                                                                                                    
atggATTGATGCCGCTCGACACAGGGCTTGATTCTTACAGTCACCCCAGCTTTGGCGGTCTATGTGGAATTTCTGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCa                                                                                                                                                      <  1:387246/149‑1 (MQ=255)
 tgaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                                                                                     >  2:44341/1‑149 (MQ=255)
 tgaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                                                                                     <  1:504015/149‑1 (MQ=255)
   aaTTAATGCCGCTGGCCAAAGGGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGTATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                                                                                   >  2:615383/1‑149 (MQ=255)
     ttAACGCCGCTCGCCACAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAc                                                                                                                                                 <  2:164696/149‑1 (MQ=255)
       aaTGCCGCTCGCCAAAGAGATTGACCCTAATAATACCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACaa                                                                                                                                               <  2:842575/149‑1 (MQ=255)
        acgCCGCTCGACAAAGAGATTGACTCTAATAATCACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAAc                                                                                                                                              <  2:723273/147‑1 (MQ=255)
            cGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTGTGTGGAATGTCAGTGATGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGCAGTTGTCAAGCGCATGTACaaagtct                                                                                                                                          >  2:228513/1‑144 (MQ=255)
              cTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAAAGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                                                                        >  1:556438/1‑149 (MQ=255)
                    aaaGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCtt                                                                                                                                  >  2:89749/1‑149 (MQ=255)
                      agagATTGACCCTAATAATACCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACTGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                                                                                <  2:338698/149‑1 (MQ=255)
                       gctatggACGCTAATAACACCCCGCGCTATGGCGTACGATGTTGAATGTCAGTGATGGGAGTCAGTTGAGTGCTGCCACAAACGGCGGGAATTGGTTAAAAACAACGTCCGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                                                                               <  1:753770/143‑1 (MQ=255)
                        agaTTGACCCTACTACTCTCCCTAGCTATGGCGTACTATGTGGAAGGTCAGTGATGGGATTCAGTTTAGTTCTGCTACTAACGGCGGGAATTGGTTAAAAACAACAGCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgttgttttt                                                                                                                              <  1:684527/149‑12 (MQ=25)
                        agaTTGACCCTAATGATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACAGCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgttgttttt                                                                                                                              <  1:717541/149‑12 (MQ=37)
                          aTTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCTGCTTCACGaa                                                                                                                            >  2:329187/1‑149 (MQ=255)
                             gACCTTAATGATAACCCCAGCTAGGGCCTACTATGTGGAATGTCAGTGATGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGc                                                                                                                         <  1:146718/149‑1 (MQ=255)
                                ccTAATAGTAACGCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTTTCCAGCGCATGGACAACTTCTACCAGCATCACGaaacactt                                                                                                                      >  1:58630/1‑144 (MQ=255)
                                ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTTCTACACACGGCGCGTATCTGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTACCAGCTTCCCGCAAGCCtt                                                                                                                      >  1:742277/1‑149 (MQ=255)
                                ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                                                                      >  1:160460/1‑149 (MQ=255)
                                ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGATTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCACCTGCACTAAATCCtt                                                                                                                      >  1:564585/1‑149 (MQ=255)
                                       ataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCCGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTCATTCTag                                                                                                               >  1:432028/1‑148 (MQ=255)
                                        taaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                                                                              <  2:380205/149‑1 (MQ=255)
                                                  tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                                                                                    >  2:740729/1‑149 (MQ=255)
                                                   aTGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGt                                                                                                   <  2:780980/149‑1 (MQ=255)
                                                     gccgtcccgtgttGAATGTCGGTGATGTGATTCAGCTTAGTGGTGCTAGCACCGGAGGGAATTGGTTAAAATCTACATCAGAAGTGGTCCAGCGCATGGACAACTTCTGCCAGCGTCACGAAAGCCTTATTCTACCTTTTCGGCTGtct                                                                                                 <  1:422740/140‑1 (MQ=255)
                                                         tACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                                                                             >  2:768969/1‑149 (MQ=255)
                                                          aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTTTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAATGCCTTATTCTACCTTTTCGGCTGtctcttcc                                                                                            <  1:635359/149‑2 (MQ=255)
                                                          aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttct                                                                                            <  2:83905/149‑1 (MQ=255)
                                                           cTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                                                                           >  2:537936/1‑149 (MQ=255)
                                                              tgtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTAATTCTAACTTTTCGGCTGTCTCTTCTCTCg                                                                                        >  2:583226/1‑149 (MQ=255)
                                                                        cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                                                                              >  1:130778/1‑149 (MQ=255)
                                                                         aGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                                                                             >  2:695025/1‑149 (MQ=255)
                                                                             aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                         >  2:735032/1‑149 (MQ=255)
                                                                             aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                         <  1:291755/149‑1 (MQ=255)
                                                                             aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                         >  2:39092/1‑149 (MQ=255)
                                                                              tGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGTTTAACAACAACATCAGAAGTTGTCCAGCGCATGGACTTCCTATCCTACCGTCACTGGAGCCTTTTTCTACCTTTTCGGCTCTCTCTCCTCTCGTACTGTTTACGTAttt                                                                        <  1:401114/149‑1 (MQ=255)
                                                                                ggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAACTTTTCTGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                                                                      >  2:377421/1‑149 (MQ=255)
                                                                                ggAATCAGCTTAGGTCTGCTACAAACGGCGGGAATTGGTTAGAAACAAGATGAGGAGTTGTCCAGAGCATAGACAACTTCTACCAGCCTCACCCAAGCCGTATTCTACATTTTCTGCTGTCTCCTTTCTCGCACTGTTTAAGTCttttt                                                                      >  1:61557/1‑147 (MQ=255)
                                                                                   ttCCGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTCAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTCCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc                                                                   <  2:566920/149‑1 (MQ=255)
                                                                                    tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                                                                  >  2:826576/1‑149 (MQ=255)
                                                                                     cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc                                                                 <  1:259498/149‑1 (MQ=255)
                                                                                            gTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTACCGCGCTTCaa                                                          >  2:59719/1‑149 (MQ=255)
                                                                                                 gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGCCAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAATTGTTCCGCGCTTCAAACAGt                                                     >  1:41120/1‑149 (MQ=255)
                                                                                                   tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGc                                                   <  1:810688/149‑1 (MQ=255)
                                                                                                     cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGt                                                 <  2:675023/149‑1 (MQ=255)
                                                                                                      aaaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGtc                                                >  2:199769/1‑149 (MQ=255)
                                                                                                       aaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGtct                                               <  1:740729/149‑1 (MQ=255)
                                                                                                           gcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGt                                           >  2:105929/1‑149 (MQ=255)
                                                                                                                     tttAAAAACAACATCAGAAGGTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGTCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTCAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGaaa                                 <  1:195584/148‑1 (MQ=255)
                                                                                                                       tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGGAACg                               >  1:393939/1‑149 (MQ=255)
                                                                                                                          aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGGGTCTCTGTCCTGGCGGATCGccc                            >  1:709025/1‑149 (MQ=255)
                                                                                                                           aagaaaaTCAGAGGTTGTCCAGCGTGGGGAGATATGCTGAAAGGTTGAGGGAGGCCTTATTGTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTGTAAGTATTTGTTCTGGGCTTCAAGCAGTGCGTTTCTGTCCTGGCGATACGccgg                           >  1:79718/7‑147 (MQ=255)
                                                                                                                              gctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACa                        >  2:404475/6‑149 (MQ=39)
                                                                                                                                  tCAGAGGTTGTCCCGCGCATGGACCACTTCTGCCAGCTTCCCGAATGCCTTCTCCTACCTCTCCGCCTGTCTCTTCTCTCGTACTGTTTACGTATTTGTTCCGCGCTGCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCTCGACAATaa                    <  1:651996/149‑1 (MQ=255)
                                                                                                                                   cAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCGTCAAGCAGTGCGTCTCTGTCCTGGCGAGACGCCCGACAGTAAc                   >  1:290990/1‑149 (MQ=255)
                                                                                                                                       aGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGGAACGGCCGACACTAACGttt               >  1:607058/1‑149 (MQ=255)
                                                                                                                                       aGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAAGAACGttt               >  2:862783/1‑149 (MQ=255)
                                                                                                                                        gTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGCAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGttta              >  2:316636/1‑148 (MQ=255)
                                                                                                                                            tCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAACCTTATTCCACCCTTTCGGCTGTCTCTCCTCACGTACTGGTTAAGTCGTTGCTCCGCGCATGAAGCAGTCCGTCGCTGTCCTGCCGAAACGCCCGACAATAACGTTGCacag          >  1:733819/1‑146 (MQ=255)
                                                                                                                                             ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGCCAATAACGTTTCAAATg         >  1:73929/1‑149 (MQ=255)
                                                                                                                                             ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATg         >  1:805534/1‑149 (MQ=255)
                                                                                                                                              cAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGc        >  2:317253/1‑149 (MQ=255)
                                                                                                                                                 gcgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAg      >  1:562635/1‑149 (MQ=255)
                                                                                                                                                 gcgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCAACAATAACGTTTCAAATGCAg      <  2:834881/149‑1 (MQ=255)
                                                                                                                                                     aTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGaaa  <  2:187902/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAA  >  CP009273/3176561‑3176857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: