Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 3,178,254 IS5 (+) +4 bp 74.0% coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)36 (0.730) 27/274 NT 72.9% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 3178254 = 13 (0.270)coding (56/291 nt) yqiC BMFP family putative fusogenic protein
* ? CP009273 = 2283592NA (NA)38 (0.760) 32/276 NT 73.9% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 3178257 13 (0.270)coding (59/291 nt) yqiC BMFP family putative fusogenic protein

GGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGT  >  CP009273/2283446‑2283740
                                                                                                                                                  |                                                                                                                                                    
gggACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCAt                                                                                                                                                    <  1:863050/149‑1 (MQ=31)
cgaacaggcaaaTCTGTTTTCTTGTCTTAGCGCGCTTACTGCTCCATTCACTGTCGGCGAAGGTAAGTTGATCACTCAGGATGAACCCTGTTCTATGGCGCCAGATGACAAACATGATCTCATATCATTGACTTGTTCGCACCTTCCAt                                                                                                                                                    <  1:679773/140‑1 (MQ=11)
    cAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACg                                                                                                                                                <  2:635552/149‑1 (MQ=34)
     aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGc                                                                                                                                               <  2:322815/149‑1 (MQ=35)
        aaacTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                            <  2:49832/149‑1 (MQ=38)
        aaaaTCTCTTGTCTGGTCTGACGGCGCTGACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                            <  1:504995/149‑1 (MQ=255)
              tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                                      <  2:179853/149‑1 (MQ=255)
              tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                                      >  1:292442/1‑149 (MQ=255)
              tctTGTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                                      <  2:433318/149‑1 (MQ=255)
                ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTAGCACCTTCAATACCGCAGAACccacca                                                                                                                                    >  1:849935/1‑149 (MQ=11)
                    cTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAAc                                                                                                                                <  2:306767/149‑1 (MQ=255)
                     tGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACCTGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACa                                                                                                                               <  1:559158/149‑1 (MQ=255)
                       gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGa                                                                                                                             >  1:733337/1‑149 (MQ=255)
                          tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGatat                                                                                                                          >  2:309124/1‑149 (MQ=255)
                            aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTg                                                                                                                        >  2:97939/1‑149 (MQ=255)
                             cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGc                                                                                                                       <  2:614876/149‑1 (MQ=255)
                              ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCg                                                                                                                      <  1:627187/149‑1 (MQ=255)
                               gcgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATAATGccg                                                                                                                     >  1:498301/1‑147 (MQ=255)
                                       tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaa                                                                                                             >  1:768503/1‑149 (MQ=255)
                                        gctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                            <  1:606535/149‑1 (MQ=255)
                                            aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATCCACCCTGTTCTCTGGCTCCACATGACAAACATCACCTCATACCCGCGACTTCTTCGCACCTCCCATAACGCCCTAGCCACCCGACCAGCTCTTACGGACCGCAACAgaga                                                                                                        >  1:174909/1‑149 (MQ=9)
                                              ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaaa                                                                                                      <  2:337791/149‑1 (MQ=255)
                                              ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaaa                                                                                                      >  1:39182/1‑149 (MQ=255)
                                                        gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACGGATATTGCGGAACGAGGAAGAGAAACCGAACCAGa                                                                                            >  2:75427/1‑149 (MQ=255)
                                                        gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGa                                                                                            >  2:148080/1‑149 (MQ=255)
                                                            aGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGa                                                                                        <  2:292442/149‑1 (MQ=255)
                                                              gTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATg                                                                                      >  2:635632/1‑149 (MQ=255)
                                                                    tgatgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACGGATATGGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGa                                                                                >  1:487046/1‑149 (MQ=255)
                                                                          ctctagaggaACCCTGTTCTATTGGTCCAGATGACGGACAGTGTCTCATATTGGGCACTTGTTCGCACCTTCCATAACGCTGCAGCCATCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTc                                                                          <  1:738109/141‑1 (MQ=255)
                                                                          cgcATGATGAACCCTGTTCTATGCTGCCAGATCACAATCATTATTTCGTATTATGGAGTGGTGGGCTGCTTCGATATGTGTGTAGTCAGCAGAACAGTTATGGCGGAACTGCAAGTGCAGACAGAACCAGATTGTTGCATTTAGCGTTc                                                                          <  1:238115/147‑1 (MQ=255)
                                                                           tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCa                                                                         >  1:733803/1‑149 (MQ=255)
                                                                           tCATGATGAACCCTGTTCTATGGCTACAGATGACAAACATGATCCCATAACAGGGACTTGTTGGCACCTTCCATAACGCTGTACCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATCGACGCATTGAGCTTTcc                                                                         >  1:252214/1‑148 (MQ=25)
                                                                               gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCt                                                                     <  2:733337/149‑1 (MQ=255)
                                                                                  gaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATg                                                                  >  2:263050/1‑149 (MQ=255)
                                                                                     cccTGTTCTATGGATCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGaaa                                                               >  1:533801/1‑149 (MQ=255)
                                                                                          ttCTATGGATCCAGATGGCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCTGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaat                                                          <  2:604848/149‑1 (MQ=255)
                                                                                              aTGGCTCCAGATGACAAACACGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCt                                                      <  2:395564/149‑1 (MQ=255)
                                                                                              aTGGCTACAGATGACAAACAAGGGCTCATATCAGCGACTTGTAAGAACCATCCAGAAAGTTGTAGCAACGAGAACACATATGGAAGAACGCCAAAGAGTAACAGAACCAGAATGATGCATTGAGCTTTCATCCTAAGAAATTAATAGCt                                                      >  1:361551/1‑149 (MQ=255)
                                                                                                  cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTa                                                  <  1:309124/149‑1 (MQ=255)
                                                                                                    ccAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaa                                                >  2:704247/1‑149 (MQ=255)
                                                                                                     cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                               <  1:862409/149‑1 (MQ=255)
                                                                                                     cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                               >  1:811677/1‑149 (MQ=255)
                                                                                                       gATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGAATGATGCCGTGAGCTTTCAGCCTATGAAATTAATTGCTGTTAAAAGc                                             >  2:590075/1‑149 (MQ=255)
                                                                                                          gACAAACATGATCTCATATCAGGGACTTCTTCCCACCTTCCATAACGCTGTAGCCACCAGAACAGATCTTGCGGCACGACAAAGAGAAACAGAACCAGCTTGATGCATGGACATTTCATCCTATGAAATTAATTGcgttgcaaaccatt                                          >  1:317554/1‑136 (MQ=37)
                                                                                                            cAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTgg                                        <  2:840297/149‑1 (MQ=255)
                                                                                                                  tGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAg                                  <  1:148080/149‑1 (MQ=255)
                                                                                                                   gATCTCATATCAGGGACTTGTTCGCACCTTCCATAACTCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGa                                 <  2:307623/149‑1 (MQ=255)
                                                                                                                    aTCTCATATCAGGGACTTGTTCCCACCTTCCATAACGCTGTAGGCACCAGAACAGATATTGCGGAACGACAAAGAGAAACCGGACCAGATGGAGGCATTGAGCTTCCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaa                                <  1:185351/149‑1 (MQ=255)
                                                                                                                      ctcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaaaa                              <  2:533801/149‑1 (MQ=255)
                                                                                                                      cccATATCAGGGACTTGTGCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGCACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaaaa                              <  1:248152/147‑1 (MQ=255)
                                                                                                                           atCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAccc                         >  1:169060/1‑149 (MQ=255)
                                                                                                                             cAGGGACCTGTTCGCAGCTTCCATAACGCTGTAGCCACCAGAACAGATGTTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCAt                       <  2:286540/149‑1 (MQ=255)
                                                                                                                              aGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTCAAAGCATTGGGTACAGAAAATACCCATa                      <  2:481341/149‑1 (MQ=255)
                                                                                                                              aGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGGAAATACCCATa                      >  2:295084/1‑149 (MQ=255)
                                                                                                                              aGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTAAAAAGCATTGGGTACAGAAAATACCCATa                      >  2:236563/1‑149 (MQ=255)
                                                                                                                                    ttGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCa                >  2:310234/1‑149 (MQ=255)
                                                                                                                                      gTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACACGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAACACCATa              >  1:388904/1‑149 (MQ=255)
                                                                                                                                       ttCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAc             >  1:435155/1‑149 (MQ=255)
                                                                                                                                                  cATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGGCCCATACCCGGCGACAGt  >  1:257476/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGT  >  CP009273/2283446‑2283740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.