Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,717,970 G→C 9.4% A379G (GCG→GGG)  kgtP ← alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,717,9700GC 9.4% 191.3 / 8.2 69A379G (GCG→GGG) kgtPalpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (22/39);  new base C (5/2);  total (28/41)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTG  >  CP009273/2717822‑2718115
                                                                                                                                                    |                                                                                                                                                 
cttcccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCg                                                                                                                                                   <  1:35303/149‑1 (MQ=255)
       ttGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGGCCGCATATGa                                                                                                                                            >  1:680312/1‑149 (MQ=255)
       ttGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCAGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGGACCACCAAATATAGGATTAGCGACCGCATATGa                                                                                                                                            >  1:468069/1‑149 (MQ=255)
        tgggctGTTGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGAc                                                                                                                                           >  1:365909/6‑149 (MQ=255)
           gATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATAAGACAGa                                                                                                                                        >  1:310495/1‑149 (MQ=255)
               tAGCATCAAAGAACCCAAAAACGCCACCCCGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGTCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCaa                                                                                                                                    <  2:132493/149‑1 (MQ=255)
               tAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCaa                                                                                                                                    <  1:254399/149‑1 (MQ=255)
                  cATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGc                                                                                                                                 <  1:384809/149‑1 (MQ=255)
                  cATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCCCATATGACAGACCACCGc                                                                                                                                 >  2:130577/1‑149 (MQ=255)
                      aaaGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTaa                                                                                                                             <  1:544670/149‑1 (MQ=255)
                      aaaGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTaa                                                                                                                             <  2:371117/149‑1 (MQ=255)
                       aaGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAAt                                                                                                                            <  1:388999/149‑1 (MQ=255)
                          aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATgcg                                                                                                                         <  2:749142/149‑1 (MQ=255)
                          aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCCCCTATGACAGACCAACGCCTAAAgcg                                                                                                                         >  1:240521/1‑149 (MQ=255)
                          aaaCCAGAAACGCCACCACGGCCATCAAGGTCACACACCAGAAGCAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATgcg                                                                                                                         <  1:856310/149‑1 (MQ=255)
                           aaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATgcgc                                                                                                                        <  1:845292/149‑1 (MQ=255)
                           aaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATgcgc                                                                                                                        <  2:72240/149‑1 (MQ=255)
                           aaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATCAGCGACCGCATATGACAGACCAACGCCTAATgcgc                                                                                                                        <  1:41798/149‑1 (MQ=255)
                                     gCCACCCCGGCCATCAAGGTCACGTACCAGAAGAAGCCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACCGACGAGGGTGTAATGCGCGAAGCTGTGc                                                                                                              <  1:192360/149‑1 (MQ=255)
                                     gCCACCACGGCCGTCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAATGCCTAATGCGCGAACCTGTGc                                                                                                              >  2:626682/1‑149 (MQ=255)
                                     gCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGc                                                                                                              <  1:764529/149‑1 (MQ=255)
                                         ccacGGCCATCAAGGTCACATACCAGAAGAAGGCTGCTTCCATACCGATGCATTTCACCGACAACGCCACGTGCTCCGCCGAACCAACAAATATAGCATTAGCGACCACATATGACAGACCACGGCTCACTGCGCGAACCTCAGCCggg                                                                                                          >  1:101730/1‑149 (MQ=255)
                                          cacGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGa                                                                                                         >  2:491032/1‑149 (MQ=255)
                                           acGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCCAATATCAGACCAACGCCTAATGCGCGAACCTGTGCCGGGaa                                                                                                        <  2:424826/149‑1 (MQ=255)
                                           acGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGaa                                                                                                        <  2:225454/149‑1 (MQ=255)
                                              gCCATCACGTTCACATACCAGAAGATGGCTGTTTCCATTCCGATTGATTACAGCGACTACGCGACGTACTCCGCCGAACCATCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACAt                                                                                                     <  2:722961/149‑1 (MQ=255)
                                                cATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGTCCGCATATGACGGCCCAACCCCTAATGCGCGAACCGGTGCCGGGGACAtcc                                                                                                   >  1:229524/1‑148 (MQ=255)
                                                          acaTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAg                                                                                         >  1:466337/1‑149 (MQ=255)
                                                             tACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCACCGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTAt                                                                                      >  2:58051/1‑149 (MQ=255)
                                                              aCCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGCAtt                                                                                     >  2:814412/1‑149 (MQ=255)
                                                                   aagaagGCTGTTACCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACACCTCAGCCTTCAGTATTCCACt                                                                                <  1:628306/149‑1 (MQ=255)
                                                                        ggCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAAGATAGCATTAGCGACCGCATATGACAGACCAACGCCGAATGCGGGAACCTGTGCCGGGAACATCTCCGCCTTCAGCATTCCACtgattg                                                                           >  1:852357/1‑149 (MQ=255)
                                                                         gCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATCAGCGACCCCATATGACACACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtgattga                                                                          <  1:454408/149‑1 (MQ=255)
                                                                          cTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtgattgat                                                                         <  2:274052/149‑1 (MQ=255)
                                                                          cTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtgattgat                                                                         <  1:323501/149‑1 (MQ=255)
                                                                          cTGGTTCCATTCCCACTGATTGCAGCGACAACGCTACGTCCTCAGATGATCCCCCAAAGAGGGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGTAACATCTCAGCCTTCAGTATTCCACtgattgat                                                                         <  2:166046/149‑1 (MQ=255)
                                                                              ttCCATTCCTCTTGATTTCAGCCACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGtat                                                                     <  2:229757/149‑1 (MQ=255)
                                                                                ccATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaa                                                                   <  2:365909/149‑1 (MQ=255)
                                                                                   ttCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaaaaa                                                                <  2:549667/149‑1 (MQ=255)
                                                                                   ttCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaaaaa                                                                <  2:549634/149‑1 (MQ=255)
                                                                                      cTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCCCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGCTGTATaaaacatc                                                             >  1:436190/1‑145 (MQ=255)
                                                                                       tATTGATGTCCGCGACAACGCTACGTACTCCGCCGAGCCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCa                                                            <  1:736221/149‑1 (MQ=255)
                                                                                              ttCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcag                                                     >  1:855302/1‑149 (MQ=255)
                                                                                                 cgccaCAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGACCCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagcag                                                  <  2:465687/145‑1 (MQ=255)
                                                                                                 aGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagcag                                                  <  2:592543/149‑1 (MQ=255)
                                                                                                   ccaCAGCGCTACGTCCTCCGCCGAACCACCGAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAggg                                                <  1:211821/147‑1 (MQ=255)
                                                                                                     aCAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCCCTGATTGATGTATAACAACTCACTATCAGCATGGca                                              <  1:858388/149‑1 (MQ=255)
                                                                                                      cAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGACGGGAACGCCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac                                             <  1:746406/149‑1 (MQ=255)
                                                                                                         cGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGAATAAAAACTCACTATCAGCAGGGcacaca                                          >  1:3472/1‑149 (MQ=255)
                                                                                                         cGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAAACTGTGCCGGGAACATCCCAGCCTTCAGTATTCCACTGCTTGATGTATAAAAACTCACTATCAGCAGAGcacaca                                          >  1:65565/1‑149 (MQ=255)
                                                                                                             aCGTGCTCCGCCGAACCACCAAATATAGCATTAGCGACCCCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCAc                                      <  1:130577/149‑1 (MQ=255)
                                                                                                              cGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGAGGCATAAAAACTCACTACCAGCAGGGCACCCATCAcc                                     >  2:71007/1‑149 (MQ=255)
                                                                                                                tACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAg                                   <  2:97224/149‑1 (MQ=255)
                                                                                                                tACTCCGCCGAACCACCAAATATAGCATTAGCCACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAg                                   <  1:265478/149‑1 (MQ=255)
                                                                                                                 aCTCCGCCGAACCACCCAATATATCATCTGCGACCGCATACGACACACCATCGCCTATCGCGCGAACCTGTGACTGGAACACCTCAGCCTCCAGTATCCCACTGATTGATGCATAAAAACTCACTATCAGCAGGGCACACCTCACCata                                  <  1:277810/149‑3 (MQ=255)
                                                                                                                   tCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGGATTCCACTGATTGATGTATAAAAACTCACTACCAGCAGGGCACACATAACCAGAcc                                >  1:636435/1‑149 (MQ=255)
                                                                                                                    ccgccgAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCa                               >  2:656091/1‑149 (MQ=255)
                                                                                                                      gccgAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCaaa                             >  2:383122/1‑149 (MQ=255)
                                                                                                                         gAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGc                          <  2:709501/149‑1 (MQ=255)
                                                                                                                            ccaccaAATATAGCCTTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGGGCCGGGAACATCTCAGCCTTCAGTATTTCACTGATTGATGTATAAAACCTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGc                       <  1:50451/149‑1 (MQ=255)
                                                                                                                            ccaccaAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTGCACTGATTGATGTATAAAAACTCCCTATCAGCAGGGCACACATCACCAGACCAAAAGCGGc                       <  1:473126/149‑1 (MQ=255)
                                                                                                                              accaAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGCTTGATGTATAAAAACTCCCTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCAt                     >  1:575634/1‑149 (MQ=255)
                                                                                                                                        gCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTCATTGATGTATAAAAACTCACTATCAGCAGGGCACACACCACCAGACCAAAACCACGATAAGGAGAGGa           >  1:462562/1‑149 (MQ=255)
                                                                                                                                         cATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATAACCAGACCAAAGGCGGCATAAGGCGAGGaa          >  2:321933/1‑149 (MQ=255)
                                                                                                                                         cATTAGCGACCGCATATGACAGACCAACGCCTAAGGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTAGTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATACGGCGAGGaa          >  1:813411/1‑149 (MQ=255)
                                                                                                                                          aTTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCCCAGCCTTCAGTACTCCACTGAGTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGaaa         <  2:466337/149‑1 (MQ=255)
                                                                                                                                               cGACCCCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTACCAGCAGGGCAACCATCACCAGACCCAAAGCGGCATAAGGCCAGGAAACGttt    >  1:142447/1‑149 (MQ=255)
                                                                                                                                               cGACCCCATATGACAGACCAACGCCTAAGGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCACAAGGCGAGGAAACGttt    >  1:202859/1‑149 (MQ=255)
                                                                                                                                                 aCCGCATGTGACAGACCAACGCCTAAGGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGAGCAAGTGCGGCATAAGGCGAGGAAACGTTTTg  <  1:190122/149‑1 (MQ=255)
                                                                                                                                                 aCCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCCCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTg  <  2:347762/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTG  >  CP009273/2717822‑2718115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑