Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,029,079 C→T 38.2% A188T (GCT→ACT)  prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,029,0790CT38.2% 56.5 / 62.4 72A188T (GCT→ACT) prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base C (19/25);  new base T (15/12);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTT  >  CP009273/3028942‑3029222
                                                                                                                                         |                                                                                                                                               
ttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGCACGCCGGGTTCTGTACGCAGCCAGAAGTAAGTGTAATCGCCgg                                                                                                                                      >  1:352874/1‑149 (MQ=255)
ttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCgg                                                                                                                                      >  1:470859/1‑149 (MQ=255)
ttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTAACGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGGGACCGCCGGTTGCTGTACGCAGCGAGGCGTAGGGGTAATCGCCgg                                                                                                                                      >  2:245004/1‑149 (MQ=255)
 tCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACACACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGga                                                                                                                                     <  2:815082/149‑1 (MQ=255)
  cGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCgggg                                                                                                                                    >  2:212612/1‑147 (MQ=255)
  cGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAACGTAATCGCCggga                                                                                                                                    >  2:52769/1‑147 (MQ=255)
   gATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGgaga                                                                                                                                   <  1:802176/149‑1 (MQ=255)
      atcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCCGCCCGCCGTAAGTGTAATCGCCGGAGAttt                                                                                                                                >  2:58808/1‑149 (MQ=255)
       tcaatatcaTCATCAACTTCCGGATAATCAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGTTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGAtttt                                                                                                                               <  2:494461/149‑1 (MQ=255)
       tcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTAAACCGAGCTTTTACTGACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGAtttt                                                                                                                               <  1:219547/149‑1 (MQ=255)
         aatatcaACATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGa                                                                                                                             <  2:228590/149‑1 (MQ=255)
          atatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGAt                                                                                                                            <  2:23474/149‑1 (MQ=255)
          atatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGAt                                                                                                                            >  1:674903/1‑149 (MQ=255)
           tatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATc                                                                                                                           <  2:278357/149‑1 (MQ=255)
           tatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATc                                                                                                                           <  2:303223/149‑1 (MQ=255)
           tatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATc                                                                                                                           <  2:345345/149‑1 (MQ=255)
           tatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATc                                                                                                                           <  2:508097/149‑1 (MQ=255)
             tcatcatcaACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGt                                                                                                                         <  2:865411/149‑1 (MQ=255)
                       cTTCCGGATAAACCAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTa                                                                                                               <  2:755744/149‑1 (MQ=255)
                        ttCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTGTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGACCGTTTGAGCGGCACGGGttgac                                                                                                              >  1:468515/1‑146 (MQ=255)
                        ttCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCCTTTACGCACCAGGCGGCGAACGCCGGTTTCCGTACGCAGCCACCCCTAAGCGTAATCGCCGGAGACTTTGATCGCCCCGCACTTaa                                                                                                              >  1:50665/1‑149 (MQ=255)
                         tCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTAAAt                                                                                                             >  1:535991/1‑149 (MQ=255)
                          ccGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATa                                                                                                            >  2:776072/1‑149 (MQ=255)
                           cGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATAc                                                                                                           <  2:336222/149‑1 (MQ=255)
                            ggATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTATACACGGCCAGCCGTAAGCGTAATCTCCGGAGATTTTGATCGTCACGGCTTTAATAcc                                                                                                          >  1:478878/1‑149 (MQ=255)
                              aTAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCg                                                                                                        <  1:120172/149‑1 (MQ=255)
                                    aaacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAGTACCCGCCACtg                                                                                                  >  1:625753/1‑148 (MQ=255)
                                          ggAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTACGTGTGATCGCCGGCGCTTTTGCTCGTCACGGATTTAATACCCCCCACGTCCCCtt                                                                                            >  1:688842/1‑149 (MQ=255)
                                          ggAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGGTTTTGATCGCCACGGAATTAATACCCACCACTTCACCtt                                                                                            >  2:108985/1‑149 (MQ=255)
                                           gAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTc                                                                                           <  2:778717/149‑1 (MQ=255)
                                            aGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATCTAATACCCGCCACTTCACCTTcc                                                                                          <  2:569133/149‑1 (MQ=255)
                                                 aaCGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCCGAGAGTTTGATCGTCCCGGATTTAATACCCGCCACTTCACCTTCCGCctc                                                                                     >  1:146368/1‑149 (MQ=255)
                                                  acgacgTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGActct                                                                                    >  2:331473/1‑149 (MQ=255)
                                                   cgacgTGTGCCGACGACCGCGGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCtt                                                                                   <  1:686499/149‑1 (MQ=255)
                                                      cgTGGGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATCTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCga                                                                                <  1:567378/149‑1 (MQ=255)
                                                           ggCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTCCGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGAtt                                                                           <  2:106700/149‑1 (MQ=255)
                                                              gacgacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                        >  2:150343/1‑149 (MQ=255)
                                                                 gacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtt                                                                     >  2:500601/1‑149 (MQ=255)
                                                                 gacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATAGTCACGGATTTCATACCCGCCACTTGACCTTCCGACTCTTCGATCATTTccggt                                                                     >  2:55047/1‑145 (MQ=255)
                                                                      ccgGGGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGaa                                                                <  1:34097/149‑1 (MQ=255)
                                                                          gttCAAACGGGGTTTTCCGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCa                                                            <  1:348606/147‑1 (MQ=255)
                                                                              aaaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAGGCGTAATCGCCGGAGCTTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACCGTTACATGCCTTTCGGTTTGAAACCAcccg                                                        >  1:690920/1‑149 (MQ=255)
                                                                               aaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGa                                                       <  2:369413/149‑1 (MQ=255)
                                                                                 gtggCTGTTGCGCACCAGGCCGTGACCGCGGGTTTCTGGCCGCGGCCAGCGGTAGGTGTAATCGCCGGAGATTTGGCTCGTCACTGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGAtt                                                     <  1:501431/147‑1 (MQ=255)
                                                                                 cGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGTCGGGCGACCTACCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGAtt                                                     >  2:847333/1‑149 (MQ=255)
                                                                                    gCTTCTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTg                                                  <  1:866015/149‑1 (MQ=255)
                                                                                       tttACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGGCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGccc                                               >  2:767344/1‑149 (MQ=255)
                                                                                         tACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATTCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAg                                             <  2:674903/149‑1 (MQ=255)
                                                                                           cgtaacAGGCGGTGAACGCCGGTTTCTTTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcg                                           <  2:400869/144‑1 (MQ=255)
                                                                                            gCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGGTGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcgc                                          >  1:337383/1‑149 (MQ=255)
                                                                                             cACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCa                                         >  1:366898/1‑149 (MQ=255)
                                                                                                     ggTGGAGGCCGGTGCGTGTACGCAGCCGGCCGTAAGTGTAATCGGCGGAGAGTTTGATCGTCACGGATTTAATCCCCGCCACCTCACCTGCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacata                                 <  1:242102/149‑1 (MQ=255)
                                                                                                     ggTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacata                                 <  2:352874/149‑1 (MQ=255)
                                                                                                     ggTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacata                                 <  2:470859/149‑1 (MQ=255)
                                                                                                       tGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACg                               >  1:333914/1‑149 (MQ=255)
                                                                                                         aaCGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCt                             >  1:325770/1‑149 (MQ=255)
                                                                                                           cGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACAGACGCTCa                           >  1:687858/1‑149 (MQ=255)
                                                                                                             ccGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCACATACATACGCTCAAg                         >  1:260986/1‑149 (MQ=255)
                                                                                                                     tGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATGCCTACGCTCAAGCATGCTCg                 >  2:297994/1‑149 (MQ=255)
                                                                                                                      ggaCGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGc                <  2:215356/147‑1 (MQ=255)
                                                                                                                      gTACGCAGCCAGCCGTAAGTGTAATGGCCGGAGATTTTGATCGTCACAGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGc                >  2:487275/1‑149 (MQ=255)
                                                                                                                      gTACGCAGCCAGCCGTAAGTGTAAGCGCAGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTTCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGc                >  2:649521/1‑149 (MQ=255)
                                                                                                                      gTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGc                <  1:226295/149‑1 (MQ=255)
                                                                                                                      gTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGc                <  2:59742/149‑1 (MQ=255)
                                                                                                                      gTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGc                <  2:260832/149‑1 (MQ=255)
                                                                                                                       tACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCCCAAGCATGCTCGcc               >  1:57700/1‑149 (MQ=255)
                                                                                                                         cGCAGCCGGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCa             <  1:833546/149‑1 (MQ=255)
                                                                                                                           gcgctagccGTAAGCTCAATCGCCGGAGATTTTGATCGTCACGGCTTTAATACCCGCCACTTCACCTTCCGACTCTGCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGt           <  2:44544/144‑1 (MQ=255)
                                                                                                                             gccagccGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTcc         >  1:813352/1‑149 (MQ=255)
                                                                                                                               cagccGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCtg       <  2:799969/149‑1 (MQ=255)
                                                                                                                                  ccGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGc    >  2:262862/1‑149 (MQ=255)
                                                                                                                                    gTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCtt  <  2:824897/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                               
TTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTT  >  CP009273/3028942‑3029222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑