Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,787,229 T→C 7.5% intergenic (‑186/‑118) yibB ← / → hldD YibB family protein, function unknown/ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,787,2290TC 7.5% 145.0 / 2.9 54intergenic (‑186/‑118)yibB/hldDYibB family protein, function unknown/ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base C (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

AAATATTAATAGAATGTATACTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGTTACCGGCGGCGCGGGCTTT  >  CP009273/3787084‑3787376
                                                                                                                                                 |                                                                                                                                                   
aaaTATTAATAGAATGTATACTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCa                                                                                                                                                  >  2:150312/1‑149 (MQ=255)
      tAATAGAATGTATACTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGc                                                                                                                                            <  2:519807/149‑1 (MQ=255)
            aaTGTATACTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCtt                                                                                                                                      >  2:798874/1‑149 (MQ=255)
            aaTCTATAGTGTCTATTATCCATTCTAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCCACACCCGCCATGAAGGACTAGCTAAAACCCAACCTAGTTTGTTGCAATTAGCATCCtt                                                                                                                                      <  2:168981/149‑1 (MQ=255)
                tataCTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATAGGAATATTGGTACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAACCTAGTTTGTGGCAATTAGAATCCTTGCAc                                                                                                                                  >  2:37046/1‑149 (MQ=255)
                  taCTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTCGCAATTAGCATCCTTGCACct                                                                                                                                >  2:472130/1‑149 (MQ=255)
                  taCTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCAGCCATGAAGGACTAGCTAAAACCCAAACTAGTATGTTACAATTAACATCCTTacacgc                                                                                                                                >  1:196478/1‑147 (MQ=255)
                  taCTGTATATTATCCATTCAAATGCTTCCGTTAATTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTCGCAATTAGCATCCTTGCACct                                                                                                                                >  2:558814/1‑149 (MQ=255)
                   aCTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACctc                                                                                                                               <  2:745299/149‑1 (MQ=255)
                       tataTTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCCCTATg                                                                                                                           >  2:86196/1‑149 (MQ=255)
                           ttatTCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTaaa                                                                                                                       <  2:325413/149‑1 (MQ=255)
                                 aTTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCtc                                                                                                                 >  1:724051/1‑148 (MQ=255)
                                          cctCCTTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTCTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTc                                                                                                        <  1:69758/147‑1 (MQ=255)
                                            tCCGTTATTTATCATACATCGGAATATTGATACTAAAGCGCTATTCACATGCAAAACCAACATCCGCAATGGAGGCCTAGCTAAAACCCAAACCAGTTGGTTCCAATTATCATCCTTGCACCCCTATGCAAAGGGCAGAAGGGAtcgcg                                                                                                      >  1:219406/1‑145 (MQ=255)
                                            tCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCgg                                                                                                      <  2:444738/149‑1 (MQ=255)
                                             ccGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGa                                                                                                     <  2:687322/149‑1 (MQ=255)
                                                ttatttagCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAtgt                                                                                                  <  1:93307/149‑1 (MQ=255)
                                                  atttatCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGAGATTCGGATGTGa                                                                                                <  1:604450/149‑1 (MQ=255)
                                                         aTACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGACGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGAAAAGGGCTGAAGCGATTCGCATGTGATGGTATg                                                                                         >  1:374931/1‑149 (MQ=255)
                                                          tACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCAATGTAAAGGGCTGAAGGGATTCGGATGGGATGGTAAGa                                                                                        >  2:688964/1‑149 (MQ=255)
                                                            cATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATAAAGGACTAGCGAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAATGGCTGAAGGGATGCGGATGTGATGGTATGAtt                                                                                      >  1:143612/1‑149 (MQ=255)
                                                              tCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTAc                                                                                    <  2:557540/149‑1 (MQ=255)
                                                               cGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACa                                                                                   >  2:446416/1‑149 (MQ=255)
                                                                  aaTATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGAc                                                                                >  2:626691/1‑149 (MQ=255)
                                                                    tatTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACAt                                                                              <  1:183597/149‑1 (MQ=255)
                                                                    ccctgatccgacaGCACTATTCACATGCAAAACCAGCCTCCGCCATTAAGGACTCGCAAATATCTTTTTTAGTCTGTTGCAATTAGCATCCTTGCACCTCTATTTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACAt                                                                              <  2:203286/137‑1 (MQ=255)
                                                                      ttaaTACTAAATTACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACGAGTTTGTTGCAATCAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTc                                                                            <  2:15150/146‑1 (MQ=255)
                                                                          tACTAAAGCACTATTCACATGCAAAACCAACACCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCgtgt                                                                        <  2:262228/149‑1 (MQ=255)
                                                                           aCTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTc                                                                       >  2:798195/1‑149 (MQ=255)
                                                                                aGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTgaga                                                                  >  2:212414/1‑149 (MQ=255)
                                                                                  cACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGAtt                                                                <  2:706855/149‑1 (MQ=255)
                                                                                    cTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGt                                                              <  1:472005/149‑1 (MQ=255)
                                                                                     tATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAGGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGtc                                                             <  1:731627/149‑1 (MQ=255)
                                                                                     tATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTCGCAATTAGCATCCTTGCACCTCTATGTAAAGTGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGtc                                                             <  1:558814/149‑1 (MQ=255)
                                                                                     tATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTCGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGtc                                                             <  1:472130/149‑1 (MQ=255)
                                                                                        tCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTGGTGTCTGAGATTGTgtct                                                          >  1:40011/1‑149 (MQ=255)
                                                                                          acaTGCAAAACCACCACCCGCCAGGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGa                                                        <  2:309697/149‑1 (MQ=255)
                                                                                              gCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACATACATTCGTTTCTGAGATTGTCTCTTACTcc                                                    >  2:261593/1‑149 (MQ=255)
                                                                                               cAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGCGATTGTCTCTGACTCCa                                                   >  2:634869/1‑149 (MQ=255)
                                                                                                      aaCATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCg                                            <  1:798195/149‑1 (MQ=255)
                                                                                                         aTCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAg                                         <  2:698490/149‑1 (MQ=255)
                                                                                                          tCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAgg                                        <  1:113927/149‑1 (MQ=255)
                                                                                                              ccATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTAc                                    >  2:37420/1‑149 (MQ=255)
                                                                                                                   aaGGACTAGCTAAAACACAAACTAGTTTGTTGCAATTAACATCCTTGCACCTCTATGTAAAGGGCTGAAGTGATTCGGTTGTGGTAGTATGCTTGCAGACATTGGTGGCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTa                               >  1:220317/1‑149 (MQ=255)
                                                                                                                        cTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGAGGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGatc                          <  2:751961/149‑1 (MQ=255)
                                                                                                                           gCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCCGCTCTCTAGAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGatcatc                       <  2:67086/149‑1 (MQ=255)
                                                                                                                              aaaaCCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGtt                    >  2:508715/1‑149 (MQ=255)
                                                                                                                                 aCCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCGTCGTTAcc                 >  2:837772/1‑149 (MQ=255)
                                                                                                                                 aCCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGTTAcc                 <  1:150312/149‑1 (MQ=255)
                                                                                                                                  cccAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGTTACcg                >  2:334693/1‑149 (MQ=255)
                                                                                                                                  cccAAACTAGTTTGTTACAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGGTGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGTTACcg                >  2:554523/1‑149 (MQ=255)
                                                                                                                                   ccAAACTAGTTTGTTGCAATTAGCATCCTTACACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGTTACcgg               >  2:455592/1‑149 (MQ=255)
                                                                                                                                     aaaCTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCAGCGTTACcggcg             >  2:271795/1‑149 (MQ=255)
                                                                                                                                       ccTAGTTGGGTGTAATTCGCATCCTTTCACCTCTGTGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCGCTGACTCCAGAATTCGAAGGTTACAGTTATGATCATCGTTACCggcggc           <  1:818194/148‑1 (MQ=255)
                                                                                                                                            tgtgttcCAATGAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGTTACCGGCGGCGCggg      <  1:382756/147‑1 (MQ=255)
                                                                                                                                                ctgCAATTAGCATCCTTGCGCCTCCATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGTTACCGGCGGCGCGGGCttt  <  2:724051/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AAATATTAATAGAATGTATACTGTATATTATCCATTCAAATGCTTCCGTTATTTATCATACATCGGAATATTGATACTAAAGCACTATTCACATGCAAAACCAACATCCGCCATGAAGGACTAGCTAAAACCCAAACTAGTTTGTTGCAATTAGCATCCTTGCACCTCTATGTAAAGGGCTGAAGGGATTCGGATGTGATGGTATGATTACAGACATTCGTGTCTGAGATTGTCTCTGACTCCATAATTCGAAGGTTACAGTTATGATCATCGTTACCGGCGGCGCGGGCTTT  >  CP009273/3787084‑3787376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑