Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 571,240 C→A 53.3% intergenic (‑31/+2) insH1 ← / ← nmpC IS5 transposase and trans‑activator/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273571,2400CA53.3% ‑5.9 / 78.2 62intergenic (‑31/+2)insH1/nmpCIS5 transposase and trans‑activator/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
Reads supporting (aligned to +/- strand):  ref base C (15/14);  new base A (15/18);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 7.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGCCCCATACTCCAAGATCCTTACCTT  >  CP009273/571107‑571380
                                                                                                                                     |                                                                                                                                            
tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGCTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCAtt                                                                                                                               <  1:644280/149‑1 (MQ=255)
    tttttggggcTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCATCAACGCCGTCGCACCTTACTTAGTGAAGTCATTTTTg                                                                                                                           <  1:188648/140‑1 (MQ=14)
    ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTg                                                                                                                           >  2:517874/1‑149 (MQ=40)
     ttccTGGTCTGACGGCGCTTACTGCGGGATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGt                                                                                                                          <  2:17001/146‑1 (MQ=14)
      ttCTGGTCTGACGGCGGTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTc                                                                                                                         <  1:718481/149‑1 (MQ=255)
      ttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTc                                                                                                                         <  2:697561/149‑1 (MQ=255)
        cgggTCTGCCTGCGGTTACTGCTGAATCCACTGTCGGCGACGGTCAGTTGATGACTCATGATGAACCCCGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCaa                                                                                                                       <  1:332426/147‑1 (MQ=14)
        cTGGTCTGACGGCGGTTACTGCTGAATTCACTGTCGGCGAAGGTAAGGTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCaa                                                                                                                       <  1:756592/149‑1 (MQ=255)
          ggTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGc                                                                                                                     >  1:819384/1‑149 (MQ=255)
             cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAgg                                                                                                                  <  2:736104/149‑1 (MQ=255)
                   gcgcTTACGGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATCACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGAtt                                                                                                            <  2:1707/149‑1 (MQ=255)
                     gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATAAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGAtttt                                                                                                          <  1:63920/149‑1 (MQ=255)
                           tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTGGCACCTTCCTTGGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATc                                                                                                    >  1:280372/1‑149 (MQ=255)
                           tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTGAAGCAGGTTGACTTTGTAATc                                                                                                    >  2:603957/1‑149 (MQ=255)
                           tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATc                                                                                                    >  2:387303/1‑149 (MQ=255)
                           tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATc                                                                                                    >  1:852964/1‑149 (MQ=255)
                             ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCaa                                                                                                  <  2:661553/149‑1 (MQ=255)
                             ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGGTGGTTTTGTAATcga                                                                                                  >  2:215210/1‑147 (MQ=255)
                                     aCTGGCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTa                                                                                          <  2:687268/149‑1 (MQ=255)
                                                agttAAGTTGATGACTCATGATTAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGAATTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATAttttt                                                                               <  2:184349/146‑1 (MQ=255)
                                                acgTACGTTGATGACTCCTGATGAACCCTGTTCTATGGCTTCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCACGCAGGTTGATTTTGTAATCAACGAAAGTAGACATAttttt                                                                               <  2:335169/147‑1 (MQ=255)
                                                  gTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTttgt                                                                             >  2:675668/1‑149 (MQ=255)
                                                   tAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGCTGACAAACATGATCTCATATCAGGGAATTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCATCGAAAGAAGACATATTTttgtt                                                                            >  2:819786/1‑149 (MQ=21)
                                                      tttGATGACTTATGATGAACCCTGTTCTATGGCTCCAGCTGACAAACATGATCTCATATCAGGGACTTGTTCGCACCCTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGaa                                                                         <  2:100385/148‑1 (MQ=255)
                                                      gTTGATGCCTCATGATGAACCCTGTTCTATGGCTCCAGATGACTAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGaa                                                                         <  1:448615/149‑1 (MQ=255)
                                                      gTTGATGACTCATGATGAACCCTGTTCTAGGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGaa                                                                         <  1:692031/149‑1 (MQ=255)
                                                       ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAg                                                                        >  2:719368/1‑149 (MQ=255)
                                                       ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAg                                                                        >  1:669678/1‑149 (MQ=255)
                                                       ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAg                                                                        >  1:767225/1‑149 (MQ=255)
                                                       ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAAACATATTTTTGTTGAAg                                                                        >  1:169425/1‑149 (MQ=255)
                                                          atgaCTCATGATTAACCCTGTTCTATGGCTCCAGATGACCAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTaa                                                                     <  2:572900/149‑1 (MQ=255)
                                                          atgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGCCATATTTTTGTTGAAGTaa                                                                     >  1:819058/1‑149 (MQ=255)
                                                             aCTCATGATGAACCCTGTTCTATTGCTTCAGATGACAAACATGATCTCATATGCGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAg                                                                  <  1:2880/149‑1 (MQ=255)
                                                             aCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTCGACATATTTTTGTTGAAGTAATAg                                                                  <  1:778029/149‑1 (MQ=255)
                                                              cTCAGGAGGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGGAGGTTGATTTTGTAAGCAACGAAAGTAGACATATTTTTGTTGAAGTAATAgg                                                                 <  2:45788/149‑1 (MQ=25)
                                                                 atgatgACCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCAACTTACTTAGTGAAGTCATTTTTGTCGAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTg                                                              <  2:745854/149‑1 (MQ=255)
                                                                  tgatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGCAAGTAGACATATTTTTGTTGAAGTAATAGGTTGc                                                             >  2:348503/1‑149 (MQ=255)
                                                                  tgatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAAACATATTTTTGTTGAATTAATAGGTTGc                                                             >  2:406963/1‑149 (MQ=255)
                                                                   tctcacccgtGTTCTACGGGTCCTGAAGCCAAACATGATCTCATATCAGGGACTTGTTCCCTCCTTACTTAGTGAAGTCTTTTTTTTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATTTTTTTGTTGAAGTAATAGGTTGCa                                                            <  1:672604/140‑1 (MQ=25)
                                                                    atgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTAATAAGGGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCAc                                                           >  2:767862/1‑149 (MQ=255)
                                                                      gaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAGTCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCt                                                         >  1:473268/1‑149 (MQ=255)
                                                                           cTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAGTCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATc                                                    >  2:433884/1‑149 (MQ=255)
                                                                           cTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATc                                                    >  1:491498/1‑149 (MQ=255)
                                                                           cTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTATTGAAGTAATAGGTTGCACCTACATc                                                    <  1:227179/149‑1 (MQ=255)
                                                                            tGTTCTATGCCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCa                                                   >  1:742448/1‑149 (MQ=255)
                                                                               tCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACa                                                <  1:853294/149‑1 (MQ=255)
                                                                               tCTATGCCTCCAGATGACAAACATGAGCTCATCTCAGGGACGTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACa                                                <  1:242321/149‑1 (MQ=255)
                                                                                                cAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTg                               >  1:385235/1‑149 (MQ=255)
                                                                                                 aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACAGCAACATATTTGACTAAGTCCTGa                              >  2:497852/1‑149 (MQ=255)
                                                                                                  aaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGAt                             <  1:664133/149‑1 (MQ=255)
                                                                                                    cATGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCg                           <  2:547651/149‑1 (MQ=255)
                                                                                                    cATGATCTCAGATCAGGGACGTGTGCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCg                           <  1:310597/149‑1 (MQ=255)
                                                                                                      tGATCTCATATCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGcc                         <  2:852964/149‑1 (MQ=255)
                                                                                                           ttatcccagtgtcttcttCTCCCCCTCTTTTGTGTAGTCACTTTTGTCAAGCAGGTTGTTTTTTTAATCAACGAAAGTAGACATAGTTTTGTTGAAGTAATAGGTTGCACCTTCTTGAGGATAGTTGACTAAGTCCTGATCGCCCCATa                    <  2:210355/137‑1 (MQ=255)
                                                                                                             atatCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGGCCCATACt                  >  1:383008/1‑149 (MQ=255)
                                                                                                               atCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGCCCCAtcctcc                >  1:501326/1‑149 (MQ=255)
                                                                                                               atCAGGGACTTGTTCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGCCCCATACTcc                >  1:434079/1‑149 (MQ=255)
                                                                                                                  aGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGcccactatttcaag             >  1:867803/1‑138 (MQ=255)
                                                                                                                     gACTTGTTCGCACCTTACGTAGTGATGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACAAAGTCCTGATCGCCCCATACTCCAAGATc          <  2:529012/149‑1 (MQ=255)
                                                                                                                           ttCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGCCCCATACTCCAAGATCCTTAcc    >  2:685788/1‑149 (MQ=255)
                                                                                                                           ttCGCACCTTACTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGCCCCATACTCCAAGATCCTTAcc    >  2:429067/1‑149 (MQ=255)
                                                                                                                             cGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGCCCCATACTCCAAGATCCTTACCtt  >  2:493227/1‑149 (MQ=255)
                                                                                                                                     |                                                                                                                                            
TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGAAGTCATTTTTGTCAAGCAGGTTGATTTTGTAATCAACGAAAGTAGACATATTTTTGTTGAAGTAATAGGTTGCACCTACATCAACATATTTGACTAAGTCCTGATCGCCCCATACTCCAAGATCCTTACCTT  >  CP009273/571107‑571380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: