Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,208,0070TG20.2% 421.7 / 22.3 200S534S (TCT→TCG) rpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base T (102/56);  new base G (2/38);  total (105/95)
Fisher's exact test for biased strand distribution p-value = 1.30e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 202 total aligned reads displayed.

AACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  CP009273/3207859‑3208155
                                                                                                                                                     |                                                                                                                                                     
  cGTATGCTGATGCCGGAAGACATGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAGTC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGc                                                                                                                                                     <  2:1684956/149‑1 (MQ=255)
        cTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCATCCTCGAGCTGCCGCTGGATTCTGCGaccac                                                                                                                                               <  2:1396904/149‑1 (MQ=255)
        cTGATGACGGAAGACAAGATCCGCAAAGTGCCGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccac                                                                                                                                               <  2:903433/149‑1 (MQ=255)
                ggAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCAAGCTGCAGCTGGATTCTGCGACCACCGACAggc                                                                                                                                       >  1:2134038/1‑147 (MQ=255)
                  aaGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtg                                                                                                                                     >  1:469166/1‑149 (MQ=255)
                    gACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCCCCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcg                                                                                                                                   >  2:110036/1‑149 (MQ=255)
                       aaGATCCGCATAGGGCTGAAGTGCGGCAAGGGGCCAGTC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATGGCGCCCTTGTTTTGTTGATTGATTCGGCGACCACCGAGAGCCtgcgtgc                                                                                                                                <  1:347898/149‑1 (MQ=255)
                       aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACAACCGTCGAGCTGCCGCTCGATTCTGCGACCACCGGAAGCCtgcgtgc                                                                                                                                >  2:734448/1‑149 (MQ=255)
                       aaGATCCGCAAAGTGCTGAAGATCGCAGATTCAGCAATC‑TCCATGGAAACGCCGATCGGTCATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGGCCCCCGAAAGCCggcgtgc                                                                                                                                >  1:1900064/1‑149 (MQ=255)
                        aGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATAGGTGATGATGAAGATTCGCATCAGGGGGATTTCATCGAGGATACCACCATCGAGCTGCCGCTGGATTCTGCGTCCACCGAAAGCCggggtgcg                                                                                                                               >  1:1841361/1‑149 (MQ=255)
                         gATCCGCGAAGTGCCGAAGCTCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTGCATCGAGGATACCCCCCTTGTGTTGTTTCAGGATTCGGCGACCACCGAGAGCCTGCGTGCgg                                                                                                                              <  1:397893/149‑1 (MQ=255)
                          aTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATGTCATCGAGGATACCACACTCGAGCTGCCGCTGGATTCTGCCACCGCCGAAAGCCTCAGTGCGGc                                                                                                                             >  1:253395/1‑149 (MQ=255)
                          aTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCAGCTGGGGGATTTCATCGAGGATACCACCCTCAAGCTGCCGCTGGATTCTGCGTCCACCGAAAGCCTGCGTGCGGc                                                                                                                             >  2:249895/1‑149 (MQ=255)
                            ccGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCGGGGCCCCCCGAAAGCCTACGTGCGGCaa                                                                                                                           >  2:1088151/1‑149 (MQ=255)
                             cGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCGGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAAc                                                                                                                          >  2:1337392/1‑149 (MQ=255)
                              gCAAAGTGCTGGAGATTGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGTTGATGAAGGTTCGCATCTGGGGGATTTCGTCGGGGATACTACGCTCGAGCTGCCGCTGGGTTCTGTGGCCGCCGGAGGCGtgcgtgcgttgccg                                                                                                                         >  1:487389/1‑143 (MQ=255)
                               cAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGTATCTGGGGGATTTCATCGAGGATACCACCCTCGGGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGGGCGGCAACGc                                                                                                                        >  1:2648391/1‑149 (MQ=255)
                                   gTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCacga                                                                                                                    <  2:1558839/149‑1 (MQ=255)
                                    tGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCAATCGGTGATGAGGCAGATTCGCATCTGGGGGATTTCATCGCGGATACCACCCTCGGGCTGCCGCTGGATCCGGCGACCACCGACAGCCAGCGTGCGGCAACCCacgcc                                                                                                                   >  1:873385/1‑147 (MQ=255)
                                      cTGAAGATCGCCAAAGAGCCAATC‑TCCAGGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCCACGCACGACGt                                                                                                                 >  1:694519/1‑149 (MQ=255)
                                       tGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTg                                                                                                                <  2:2563972/149‑1 (MQ=255)
                                        gAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGGCGTgc                                                                                                               >  2:1235751/1‑149 (MQ=255)
                                        gAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGACACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgc                                                                                                               <  2:1612360/149‑1 (MQ=255)
                                         aaGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGCGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCCGCGAGCGGCACCGcaccaccccct                                                                                                              >  1:2154639/1‑144 (MQ=255)
                                          aGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctg                                                                                                             <  2:1736399/149‑1 (MQ=255)
                                              cGCCAAGGGGCCAATCTTCCAT‑GAAACGCCGATGGGTGACGATCAAGATGCGCATCTGGGGGATTTCATCGAGGATACCACCCTCTTTTTTTTTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                         <  1:1665289/149‑1 (MQ=255)
                                              cGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                         >  1:1929030/1‑149 (MQ=255)
                                                ccAGAGAGCCAATC‑TCCACGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGTGCTTTCGCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                       <  1:1764828/149‑1 (MQ=255)
                                                ccAAAGGGCCAACC‑CCCATGGAAACGCCGCTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                       <  2:1657694/149‑1 (MQ=255)
                                                ccAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                       <  2:1870487/149‑1 (MQ=255)
                                                ccAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCGGCGGCTGGATTCTCCGACCACCGGAAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                       >  2:360363/1‑149 (MQ=255)
                                                 cAAAGAGCCAATA‑TCCATGGAAACGCCGAGCGGTGAGGCTGAAGGTTCGCATCTGGGGGATTTCCGCGCGGGCACCACCCTCGAGCTGCCGCTGGGTGCTGCGGCGACCGCAAGCCGGCGTCCGGCGCCGCGCGCCCTGCCGGCTGGcc                                                                                                      >  1:1283125/1‑149 (MQ=255)
                                                  aaaGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGGTTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATACTGCGACCGCCGAAAGCCTGCGGGCGGCAACGCACGACGTGCTGGCGGGCCt                                                                                                     >  1:1027622/1‑149 (MQ=255)
                                                        ccAATC‑TCCATGAAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCCCCCTCGAGCTGCCGCTGGATTCTGCGATCACCGAAAGCCTGCGTGCGGCCACGCACGACGTGCCGGCTTGCGTGACcgc                                                                                               >  2:1922162/1‑149 (MQ=255)
                                                         cAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcg                                                                                              >  1:2231390/1‑149 (MQ=255)
                                                            tc‑tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGTTCTTTCTATGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGt                                                                                           <  1:1254418/149‑1 (MQ=255)
                                                              ccccATCGACACCCCGATCGGTGATGGTGAACATTCCCATGTGGGTGATTTCGTCGAGGATCCCACCCTCTTTTTTCTTCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTg                                                                                          <  1:561616/147‑1 (MQ=255)
                                                                ccATGGAAACGCCGATCGGTGAGGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                        <  2:591286/149‑1 (MQ=255)
                                                                      gatcGCCGGTCGGTGATGCTGTAGATTCGCATTTGGGGGTTTTCATCGAGGATACCACCCTCTTGTTTTTGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCaaaa                                                                                  <  1:1369652/146‑1 (MQ=255)
                                                                      aaaCGCTCATCGGTGAGGATTAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCTTGCTGCTGCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCaaaa                                                                                  <  1:1788749/149‑1 (MQ=255)
                                                                             gATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGc                                                                           <  1:2442758/149‑1 (MQ=255)
                                                                                 ggcgatgatgaAGCTTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTAt                                                                       <  2:739624/146‑1 (MQ=255)
                                                                                  gTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATg                                                                      >  2:816484/1‑149 (MQ=255)
                                                                                  gTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGACACCACTCTCGAGCTGCCGCTGGATACTACATCCTCCGAAAGCCCGCGGCCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATg                                                                      <  2:2628298/149‑1 (MQ=255)
                                                                                        atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTc                                                                >  2:2447830/1‑149 (MQ=255)
                                                                                        atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGGGCGGCAACGCACGACGTGCGGGCTGGCCGGACCGCGCGTGGAGCAACAGGTCTGCGTATGCGGTTc                                                                >  1:2349741/1‑149 (MQ=255)
                                                                                        atgaAGAGTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTc                                                                <  2:2239669/149‑1 (MQ=255)
                                                                                         ggaAGATTCCCAGCTGGGGGATTTCATCGAGGATACCACCCTTGATTTTCTTAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                               <  1:300461/148‑1 (MQ=255)
                                                                                          gaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg‑g                                                             >  2:2432738/1‑149 (MQ=255)
                                                                                          gaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGAAGCAAAAGTTCTGGGTATGCGTTTCg‑g                                                             >  2:250287/1‑149 (MQ=255)
                                                                                          gaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGACAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGAGCTGCGTATGCGATTCg‑g                                                             >  1:2055832/1‑149 (MQ=255)
                                                                                           aaGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑Gt                                                            >  2:2184013/1‑149 (MQ=255)
                                                                                                   cagcttgggcagttCATCGAGGATACCACGCTCTGTGTGTCGCTGGATACGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGatat                                                    <  1:1066786/137‑1 (MQ=255)
                                                                                                    ttctcGGGGATTTCAGTGGGGATTCCACCGCTGAGTTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATg                                                   <  1:1219414/148‑1 (MQ=255)
                                                                                                          gggATTTCATCGACGATACCACTCTCGAGTTGCTGCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACAcc                                             <  1:1133180/149‑1 (MQ=255)
                                                                                                           ggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTGCG‑GGATCGATATGAACACCg                                            >  1:1523359/1‑149 (MQ=255)
                                                                                                             aTTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGAc                                          >  2:424404/1‑149 (MQ=255)
                                                                                                               ttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTa                                        <  2:145342/149‑1 (MQ=255)
                                                                                                               ttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCATATGCGTTTCG‑GTATCGATATGAACACCGACTa                                        <  2:1950680/149‑1 (MQ=255)
                                                                                                                  aTCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCCGACCGCGCGTGAAACAAAAGTTATGCGTATGCGGTTCG‑CTATCGAAATGAACACCGACTacac                                     >  1:1270720/1‑149 (MQ=255)
                                                                                                                   tcgagggtggtatccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGAAGAAAAAGTTCTGCAGATGCGTGTCG‑GTACCGATAGGACACCCGACTACCCg                                    >  1:2591784/14‑149 (MQ=255)
                                                                                                                   tcgagggtggtatccTCGAGCTGCCGCTGCCTGCAACTACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACg                                    >  2:1304276/14‑149 (MQ=255)
                                                                                                                   tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTTCTGGCTGGCCGGACCGCGCGTGAAGCAAACGGTCGGCGTGTGCGTGTCA‑GTAGCGATATACACACCGACTACACg                                    >  1:997990/1‑149 (MQ=255)
                                                                                                                   tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTAGCGATAGGAACACCGACTACACg                                    >  2:1221621/1‑149 (MQ=255)
                                                                                                                     gcggATACCACCCTCTTTCTTCCGCTTGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCt                                  <  1:1409038/147‑1 (MQ=255)
                                                                                                                     gAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATAGGAAAAACGACTACACGCt                                  >  2:474097/1‑149 (MQ=255)
                                                                                                                     gAGGATACCACCCCCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCt                                  <  2:418095/149‑1 (MQ=255)
                                                                                                                     caGGATACCACTCTGGTGTTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCt                                  <  1:1087885/148‑1 (MQ=255)
                                                                                                                      aGGATACCACCCTCGTTCTGCCGACGGATTCGGCGACCACCGAAAGCCTGCGTGAGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTg                                 <  1:953929/149‑1 (MQ=255)
                                                                                                                         aTACCCCCCTCGAGTTGCTGATGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGgaa                              <  1:734448/149‑1 (MQ=255)
                                                                                                                         aTACCACCCCTTAGCTGCCTCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGgaa                              <  1:1456926/149‑1 (MQ=255)
                                                                                                                             caccCTCGTGTTTCCTCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGgaagatg                          <  1:2586401/149‑3 (MQ=255)
                                                                                                                             caccCTCGAGCTGCCGCTGGATTCTGCGCCCACCAGTTTCCTGTTTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGGGTATGCGTTTCG‑GTGCGGGGATGAACACCGCTAACACGCTgagagacg                          >  2:642627/1‑142 (MQ=255)
                                                                                                                             caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGCTCTGAACACCGACTACACGCTGGaaggag                          >  2:2044693/1‑149 (MQ=255)
                                                                                                                             caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGaagaag                          <  2:1915273/149‑1 (MQ=255)
                                                                                                                             caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGAACGGCGTGCTGGCTGGCCTGACCGCGCGTGGAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCAATATGAACACCAACTACACGCTGGaagaag                          >  2:281541/1‑149 (MQ=255)
                                                                                                                             caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCATGCGAGCGGCAACGCACGACGTGCTGGCTGGCCAGACCGCGCGTGCAGCAAAAGTGCTGCGTGTGCGATTCG‑GTATAGACAGGAACACCACCTCCACgacgggcggag                          >  1:788420/1‑139 (MQ=255)
                                                                                                                              accCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGGTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGt                         >  2:1637777/1‑149 (MQ=255)
                                                                                                                               cccTCGAGCTGCTGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGGTCTTCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTg                        <  2:522245/149‑1 (MQ=255)
                                                                                                                               cccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTg                        <  2:1272873/149‑1 (MQ=255)
                                                                                                                                ccgctcgttGCCGAAGGATACGGCGACCACCGAACGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       <  1:1749209/141‑1 (MQ=255)
                                                                                                                                ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       <  2:2135045/149‑1 (MQ=255)
                                                                                                                                 cttgctcTGCCGCATGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTggg                      <  1:1060715/143‑1 (MQ=255)
                                                                                                                                    gAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaa                   >  2:2587962/1‑149 (MQ=255)
                                                                                                                                     tgcttctccTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                  <  1:1983053/141‑1 (MQ=255)
                                                                                                                                     aGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                  <  2:1319683/149‑1 (MQ=255)
                                                                                                                                      gCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGCGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACGACACGCTGGAAGAAGTGGGTAAAc                 <  2:692085/149‑1 (MQ=255)
                                                                                                                                       cTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGGGCGGCAACGCACGCCGTGCTGGCTGGCCTGACCGCGCGGGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACa                <  2:1900064/149‑1 (MQ=255)
                                                                                                                                         gCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTaca                                      >  2:2072515/1‑125 (MQ=255)
                                                                                                                                          ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGCGCAGCAAAAGTTCTGCGTATGCGTTTAGTGTAT‑TATATCACCCCCCCCCCCCCTCTCGAAGAAGTGGGTAAACAGtt             >  2:2605688/1‑149 (MQ=255)
                                                                                                                                          ccGCAGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGtt             <  1:434805/149‑1 (MQ=255)
                                                                                                                                            gCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGGTATGAACACCGACTACACGCGGGAACGAGTGGGTACACAGTTCg           >  1:2160809/1‑149 (MQ=255)
                                                                                                                                            gCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCGGGCCCGACCGCGCGTGAAGCAAAAGTCCTGCGTATGCGTGGCG‑GTAGCGAGGTGAACACCGACTACACGCTGGAAGCAGTCGGTAcacactgcg           >  1:2144677/1‑144 (MQ=255)
                                                                                                                                             cTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAGCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGa          >  1:1143560/1‑149 (MQ=255)
                                                                                                                                             cTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCgg          >  2:942038/1‑148 (MQ=255)
                                                                                                                                             cTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCCGGAAGAAGTGGGTAAACAGTTCGa          >  2:252323/1‑149 (MQ=255)
                                                                                                                                             cTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACAACCCGCTGGAAGAAGTGGGGAAAAGGTTCgg          >  1:1434615/1‑148 (MQ=255)
                                                                                                                                              tGGATTCTGCGCCCCCCGAAAGCCTGCGTGCGGCAACGCACGACGGGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCTCTATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc         <  2:101481/149‑1 (MQ=255)
                                                                                                                                               ggATTCCGCGACCCCCGAAAGCCTGCCGGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTCCGGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACg        <  2:21552/149‑1 (MQ=255)
                                                                                                                                                    cTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTAcc   >  2:2126605/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATC‑TCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCG‑GTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  CP009273/3207859‑3208155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑