Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 982,566 C→A intergenic (‑128/+475) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273982,5660CA98.5% 407.0 / NA 134intergenic (‑128/+475)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (73/57);  total (74/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

AATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATT  >  CP009273/982421‑982711
                                                                                                                                                  |                                                                                                                                                 
  tatTGCGCTTCATCATTATTTATTACCCTCATTGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACt                                                                                                                                              <  2:920554/149‑1 (MQ=255)
  tatTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTATTTATGTGTCTGCAGGCATCTTTCCATTAAAACt                                                                                                                                              >  1:1511282/1‑149 (MQ=255)
         cTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGttt                                                                                                                                       <  2:2526414/149‑1 (MQ=255)
          ttCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGACGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTc                                                                                                                                      >  2:2512671/1‑149 (MQ=255)
             atcatTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGTGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAACACTAACGTTTCGCt                                                                                                                                   >  1:2470934/1‑149 (MQ=255)
              tcatTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGTTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTATCGTTTAGCTa                                                                                                                                  >  1:2519358/1‑149 (MQ=255)
                attattTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTAcc                                                                                                                                >  2:2253075/1‑149 (MQ=255)
                 ttatttatTACCCTCATGGTTTTGTTTACGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTTCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCg                                                                                                                               >  2:1742420/1‑149 (MQ=255)
                  tatttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGt                                                                                                                              <  2:461167/149‑1 (MQ=255)
                      tattaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGaaa                                                                                                                          <  2:2337339/149‑1 (MQ=255)
                      tatgaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTATGTCGCTGGAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGaaa                                                                                                                          <  2:1278832/149‑1 (MQ=255)
                      cattaCCCTCATGTTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGCGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGaaa                                                                                                                          <  1:2429239/148‑1 (MQ=255)
                           cccTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCt                                                                                                                     <  1:2332311/149‑1 (MQ=255)
                           cccTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGGGTCTTTATGTGTCTGCAGGCAACTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCt                                                                                                                     >  1:2613869/1‑149 (MQ=255)
                            cctcctGGTCTTTTTTAAGACACCCGCCACTGCCGTCAATAAGTTCTGTCAAGAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTa                                                                                                                    <  1:2512671/149‑1 (MQ=255)
                            ccTCATGGTTTCTTTTATGATCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTACAACTAACGTTTCGCTACCGTGAAAGTGCTa                                                                                                                    <  2:836303/149‑1 (MQ=255)
                             cTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACCTTTCGCTACCGTGAACGTGCTAc                                                                                                                   <  1:1819508/149‑1 (MQ=255)
                              tCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACa                                                                                                                  >  2:2209088/1‑149 (MQ=255)
                                aTGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTGCATTAAAACGAACGTTTCGCTACCGTGAAAGTGCTACaaa                                                                                                                >  2:2058762/1‑149 (MQ=255)
                                   gTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGGTTCGCTACCGTGAAAGTGCTACaaagat                                                                                                             >  2:831473/1‑149 (MQ=255)
                                    ttttttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGAGAAAGTGCTACaaagata                                                                                                            >  2:37700/1‑149 (MQ=255)
                                     ttgttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTAccccgatac                                                                                                           <  1:994325/146‑9 (MQ=255)
                                                cacCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGttt                                                                                                >  1:1004725/1‑149 (MQ=255)
                                                 acCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTc                                                                                               >  2:2169384/1‑149 (MQ=255)
                                                 acCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTc                                                                                               <  2:2307605/149‑1 (MQ=255)
                                                   cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTGTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTGCCGTGAAAGTGCTACAAGGATAAAGATTTGGTTTCaa                                                                                             >  1:1994780/1‑149 (MQ=255)
                                                   cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTAACTTTATGTATCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACAGTGACAATGCTACAAACATAAAGATTTGGAATCaa                                                                                             >  1:610043/1‑149 (MQ=255)
                                                     gCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTCTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTACCGTTTCGCTACCGTGAAAGTGCTACCAAGATAAAGATTTGGTTTCaaca                                                                                           >  1:1480782/1‑147 (MQ=255)
                                                        aCTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGa                                                                                        <  2:2226093/149‑1 (MQ=255)
                                                         cTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGTTACAAAGATAAAGATTTGGTTTCAAAAAGaa                                                                                       >  1:609155/1‑149 (MQ=255)
                                                          tGCCGTCAATAAGTTCTGTCAATAAAACTTTACGTAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaa                                                                                      >  1:2575259/1‑149 (MQ=255)
                                                            ccGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATCGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaa                                                                                    >  2:2065502/1‑149 (MQ=255)
                                                             cGTCAATAAGTCCTGCCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaaa                                                                                   <  2:665509/149‑1 (MQ=255)
                                                              gTCAATAAGTTCTGTCAATAAAAATTTACGAAACTATTGATGAGAGTTCGGTGTCTTAAGGTGTCTGCAGGCATCTTTCCATTAAAACTAACGGTTCCCTACCGTGAAAGTGCTCCAAAGATAAAGATTTGGTTTCAAAAAGAAAACat                                                                                  >  1:1559561/1‑149 (MQ=255)
                                                               tCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAata                                                                                 <  2:1976287/149‑1 (MQ=255)
                                                                cAATAAGTTCCCTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAACCGAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGTTTTCAAAAAGAAAAAatat                                                                                <  2:100274/149‑1 (MQ=255)
                                                                 aaTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATg                                                                               <  1:694802/149‑1 (MQ=255)
                                                                 aaTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGGTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTGTCGCTACCGTGAAAGTGCTACAAAGAGAAAGATTTGGTCTCAAAAAGAAAAAATATg                                                                               >  1:1384023/1‑149 (MQ=255)
                                                                    caGTTCTGTCAATAAAAACTTACGGAAGTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTaa                                                                            <  1:390857/148‑1 (MQ=255)
                                                                        tCTGTCAATAAAGATTTACGGATCTATTTATCATGGTTTGGTTTCTGTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACaaa                                                                        <  1:1128618/149‑1 (MQ=255)
                                                                          tGTCAATAATAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAAGCatta                                                                      <  1:2447867/149‑4 (MQ=255)
                                                                          tGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaaaa                                                                      <  1:395073/149‑1 (MQ=255)
                                                                          agTCAATAAAACTATACGAAACTATTGATGGAATTGTCCTCTTATTATCTTTCTGCATCCATCTTTCCAATACACACACCCTTTCACTACCATTAAAGCACTACAAAGATAAAGATTTGGTTTAAAAAAGACAAAATATGCAACCaaac                                                                      <  1:37913/148‑2 (MQ=255)
                                                                            tCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAACCTAACGTTTCGCTACCGTGAAAGGGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAGCCAAAAGt                                                                    >  1:814410/1‑149 (MQ=255)
                                                                            tCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGt                                                                    >  1:369384/1‑149 (MQ=255)
                                                                            tCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGt                                                                    >  1:1655863/1‑149 (MQ=255)
                                                                              aaTAAAAATTTACGGAACTATTGAGGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaa                                                                  <  2:820071/149‑1 (MQ=255)
                                                                                  aaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAAGTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGATAAAATATGTAACCAAAAGTAAAAtt                                                              >  2:1645333/1‑149 (MQ=255)
                                                                                      tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAGATATGTAACCAAAAGTAAAATTTAAg                                                          >  2:456420/1‑149 (MQ=255)
                                                                                         aCGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGaa                                                       <  2:944445/149‑1 (MQ=255)
                                                                                         aCGGAAATATTGAAGAGAGCTTGGTGTCTATATGTGTCTGATGGCATCTTTACATTCAAACTAACGTTTAGCTAACGTGAAAGTGCTACAAAGATAAAAATTTGGCTGGATAAAAAAAAAATATGTAAACCAAAGTATAATTTAAGCaa                                                       >  1:2207414/1‑149 (MQ=255)
                                                                                           ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAACATTTCAgaacct                                                     >  2:51853/1‑144 (MQ=255)
                                                                                           ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACt                                                     >  1:871234/1‑149 (MQ=255)
                                                                                           ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACt                                                     >  2:1241012/1‑149 (MQ=255)
                                                                                            gAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACtt                                                    >  2:1749163/1‑149 (MQ=255)
                                                                                             aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCGTCTTTCCATTAAAACTAACGTTTAGCTACCGGGAAAGTGCTACAACGATAAAGATTTGGTTTCAAAAAGAAAAAATATCTCACCAAGAGAAAAATTGAGGGAAcgtg                                                   >  1:2179498/1‑146 (MQ=255)
                                                                                             aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACttt                                                   <  1:2209088/149‑1 (MQ=255)
                                                                                              aCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtg                                                  <  2:1326636/149‑1 (MQ=255)
                                                                                               cTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                                 <  2:1132561/149‑1 (MQ=255)
                                                                                               cTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                                 <  2:2466528/149‑1 (MQ=255)
                                                                                               cTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                                 <  1:2330092/149‑1 (MQ=255)
                                                                                               cTATTGATGAGAGTATAGTGTCTTTATGTTTCTGCAGGCATCTTTCCATTAAAACTAACCTTTCGCTCCCTTGAAAGTGCTACAAAGATAAAGATTTGGTAAAAAAAAAAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                                 <  1:1944122/149‑1 (MQ=255)
                                                                                                tATTGATGAGAGTTTTGTGTCGTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGATAACGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg                                                <  1:867902/149‑1 (MQ=255)
                                                                                                tATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg                                                >  1:2598190/1‑149 (MQ=255)
                                                                                                 aTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGa                                               >  2:2160712/1‑149 (MQ=255)
                                                                                                 aTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGa                                               >  2:2579218/1‑149 (MQ=255)
                                                                                                  ttGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTTGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                              >  1:1754044/1‑149 (MQ=255)
                                                                                                   tgaggaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACCTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTAAAAAAAAAAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAac                                             <  1:219123/149‑1 (MQ=255)
                                                                                                     atgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac                                           >  1:1701269/1‑149 (MQ=255)
                                                                                                      tgaGAGTTTGGTTTCTTTATGGGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACAcc                                          <  2:1601446/149‑1 (MQ=255)
                                                                                                         gagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTc                                       >  1:2376447/1‑149 (MQ=255)
                                                                                                         gagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTc                                       <  2:1882841/149‑1 (MQ=255)
                                                                                                         gagGTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTc                                       >  2:268832/1‑149 (MQ=255)
                                                                                                               ggTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt                                 <  2:397937/149‑1 (MQ=255)
                                                                                                                gtgtCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTACCGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGCTTTGGTTTCAAAACGAAAAAATACGTAAAGAAACGTAACATTTAAGGAACTTTGTGAAAACCGTCATAttta                                >  2:1866479/1‑148 (MQ=255)
                                                                                                                  gtCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCa                              >  2:25963/1‑149 (MQ=255)
                                                                                                                   tCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAGAGATAAAAATTTGGTTTCAAAAAGAAAAAATATGTGACCAAAAGTAAAATTTAAGGAACTTTGTAAAAACCGTAATGTTTCCAt                             >  1:1396437/1‑149 (MQ=255)
                                                                                                                     tttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATag                           >  1:1075787/1‑149 (MQ=255)
                                                                                                                     tttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGGGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACTAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCAttg                           >  1:2009454/1‑147 (MQ=255)
                                                                                                                      ttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATaga                          >  1:1513136/1‑149 (MQ=255)
                                                                                                                        aTGTGTCTGCAGGCATCTTTCCATTAAACCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAgaga                        <  1:586472/149‑1 (MQ=255)
                                                                                                                         tgtgTCTTCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGAc                       <  1:1790985/149‑1 (MQ=255)
                                                                                                                         tgtgGCTGCAGGCATCTTTCCATTAAATCTAACGTTTCGCTCCAGTGAAAGTGCTACAAAGATAAAGATTTGGAAAAAAAAAAAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGAc                       <  1:1532186/149‑1 (MQ=255)
                                                                                                                          gtgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACg                      >  2:2155622/1‑149 (MQ=255)
                                                                                                                           tgtCTGCAGGCATCTTTCCATTAAAACTAACGTATCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGGGACGt                     >  2:1712284/1‑149 (MQ=255)
                                                                                                                            gtCTGCCGGCACGTTTCCATGAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTGAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtg                    <  1:1000153/149‑1 (MQ=255)
                                                                                                                             tCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtga                   >  2:387211/1‑149 (MQ=255)
                                                                                                                              cTGCAGGCAACTTTCCATTAAAACTAACGTTGCGCTACCGTAAAAGTGCTACAAAGTTAAAGATTTGGTTACAAAAAAAAAAAATATGTACCCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgat                  <  1:445104/149‑1 (MQ=255)
                                                                                                                               tGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAAACAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtaatg                 >  1:350618/1‑149 (MQ=255)
                                                                                                                                 cAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTgatgat               >  2:1211267/1‑149 (MQ=255)
                                                                                                                                  aGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGGGACGGGATGata              >  2:2585713/1‑149 (MQ=255)
                                                                                                                                  aGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAATTGGTTTCAAAAAGAAAAAATAAGTAACCAAAAGTAAAATTTAAGGCAATTTGTGAAAACAGGCATATTTCCATAAAAACGCGataata              >  1:557225/1‑149 (MQ=255)
                                                                                                                                      aTCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAATAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTa          >  1:1916963/1‑149 (MQ=255)
                                                                                                                                       tCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAc         <  2:1798995/149‑1 (MQ=255)
                                                                                                                                       tCTTTCCATCAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAc         <  2:1555548/149‑1 (MQ=255)
                                                                                                                                        cTTTCCATTAAAACTAACGTTTCGGTACCGATAAAGTGCTACAAAGATAAAGATTTGGTTACCAAACGCAACAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACa        <  1:958070/149‑1 (MQ=255)
                                                                                                                                         tttCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAg       >  1:1544508/1‑149 (MQ=255)
                                                                                                                                          ttCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACAGCGTCATATTTCAATAGAGAGGTGATGATATTTACAga      >  1:520905/1‑148 (MQ=255)
                                                                                                                                             cATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTACAATATAATTAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAAt   >  2:619243/1‑149 (MQ=255)
                                                                                                                                               aTTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAAtt  >  1:1825530/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATT  >  CP009273/982421‑982711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: