Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,390,205	G→T	G34V (GGG→GTG)	hfq →	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,390,205	0	G	T	100.0%	139.6 / NA	46	G34V (GGG→GTG)	hfq	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/18);  total (28/18)

TCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAAC  >  CP009273/4390061‑4390350                                                                                                                                                 |                                                                                                                                                  tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCaa                                                                                                                                               >  2:284716/1‑149 (MQ=255)  cGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCaaa                                                                                                                                              <  2:683635/149‑1 (MQ=255)   gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAAt                                                                                                                                             >  1:146940/1‑149 (MQ=255)    aaaGGTTCAAAGTACAAATAAGCCTATAGGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATc                                                                                                                                            >  1:407056/1‑149 (MQ=255)    aaaGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATc                                                                                                                                            <  2:426136/149‑1 (MQ=255)         ttCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTc                                                                                                                                       >  2:458880/1‑149 (MQ=255)                tACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGAt                                                                                                                                <  1:671904/149‑1 (MQ=255)                   aaaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAg                                                                                                                             >  1:531724/1‑149 (MQ=255)                    aaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATGGAGTCTTTTGATCAGt                                                                                                                            >  2:257079/1‑149 (MQ=255)                          cATATAAGGAAAATAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGa                                                                                                                      <  1:351110/149‑1 (MQ=255)                           atatAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGAt                                                                                                                     >  2:729765/1‑149 (MQ=255)                                 ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                                                                               >  1:605033/1‑149 (MQ=255)                                 ggAAAAGAGACACTGGATAAAAGGCAATCTTTTCAAGATGCGGTCCGGAACGCACTGCGTCGGGAGCAGGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCATATCGAGTCTTTTGATCAGTTCGTGACCCTGtt                                                                                                               >  2:432418/1‑149 (MQ=255)                                       gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGcaaaa                                                                                                         >  1:315148/1‑149 (MQ=255)                                       gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTCaaaag                                                                                                         >  2:399851/1‑148 (MQ=255)                                         gagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTAGATGAATGTGATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAaca                                                                                                       >  1:722580/1‑149 (MQ=255)                                          agaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTGTTTGGTGAATGGTATTAAGCTGCAGGTGCACATCGAGTCTTTTGGTCAGTTCGTGATCCTGTGGAAAAccac                                                                                                      >  2:201529/1‑149 (MQ=255)                                           gaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACg                                                                                                     <  2:531724/149‑1 (MQ=255)                                              tGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCAATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATTGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACCAGGTc                                                                                                  >  2:41557/1‑149 (MQ=255)                                               ggCTAAGGGGCAATCTTTACAAGATACGTTCCTGTACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCa                                                                                                 >  2:710207/1‑149 (MQ=255)                                                gCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAg                                                                                                <  1:704245/149‑1 (MQ=255)                                                 cTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAACCACGGACAGc                                                                                               >  2:22991/1‑149 (MQ=255)                                                  tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTTGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGGCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGcc                                                                                              <  1:105438/149‑1 (MQ=255)                                                  tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGcc                                                                                              >  1:195360/1‑149 (MQ=255)                                                          aaTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                                                                      <  1:516387/149‑1 (MQ=255)                                                                 aCAAGATAAGTTCCTGAACGCACTGCGTCGGGGACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCGAGATGGTGTagatgc                                                                               >  2:88400/1‑144 (MQ=255)                                                                          gTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAttt                                                                      <  1:624210/149‑1 (MQ=255)                                                                             ccTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTa                                                                   <  2:303201/149‑1 (MQ=255)                                                                              cTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTTCAAGCAGGGGAttgcgac                                                                  >  2:700501/1‑144 (MQ=255)                                                                               tGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt                                                                 <  1:284716/149‑1 (MQ=255)                                                                                        tGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTATTGAAAAACACGGTCAGCCAGATGGTGTACAAACACGCGATTTCTACTGTTGTCCCg                                                        >  2:154400/1‑149 (MQ=255)                                                                                          cGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc                                                      >  2:535383/1‑149 (MQ=255)                                                                                                gAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc                                                >  1:715028/1‑149 (MQ=255)                                                                                                   cGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGACGGTTTACAAGCCCCCGATTTCTACTGTTGTCCCGTCTCGCCCGGt                                             >  2:357011/1‑149 (MQ=255)                                                                                                            gTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatca                                    <  1:458880/149‑1 (MQ=255)                                                                                                            ctttCCATTTTTTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTTACCAGTGCGTGATCCTGTTTAAAAACACGGTCATCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatca                                    <  2:132205/148‑1 (MQ=255)                                                                                                                      atttGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaacaac                          <  1:292575/149‑1 (MQ=255)                                                                                                                            tGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGCTTCTCATCACAGTAACAACGCCGGt                    >  1:643300/1‑149 (MQ=255)                                                                                                                             gAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTCACAACGCCGGTg                   >  2:626002/1‑149 (MQ=255)                                                                                                                             gAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTg                   >  2:664143/1‑149 (MQ=255)                                                                                                                               aTGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGc                 <  1:86228/149‑1 (MQ=255)                                                                                                                                  gTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGt              <  1:716385/149‑1 (MQ=255)                                                                                                                                    aTTAAGCTGCTAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTAc            >  1:621318/1‑149 (MQ=255)                                                                                                                                         gCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagca       <  1:569664/149‑1 (MQ=255)                                                                                                                                          cTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagcag      <  1:607111/149‑1 (MQ=255)                                                                                                                                              aaGTGCAAATCGAGTCTTATGATCAGTTCGTGAGTTCATGTAGTTACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAAc  >  1:542393/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                  TCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAAC  >  CP009273/4390061‑4390350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑