BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,718,678	C→G	G143A (GGC→GCC)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,718,678	0	C	G	100.0%	109.4 / NA	36	G143A (GGC→GCC)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (23/13); total (23/13)

TTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACAC  >  CP009273/2718542‑2718808

ttCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGGTGCATATTCTCGGc                                                                                                                        >  2:350483/1‑149 (MQ=255)

        tgtgtTGTAAAGCCACGACAACCAGTATGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGAt                                                                                                                <  1:683118/149‑1 (MQ=255)

        tgtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGGTGGCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCCACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGAt                                                                                                                >  2:723193/1‑149 (MQ=255)

            ttgtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCGGGGGGCATATTCTCGGCCAACAGATAATc                                                                                                            >  1:615749/1‑149 (MQ=255)

                aaaaCCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTg                                                                                                        <  1:620950/149‑1 (MQ=255)

                 aaaCCACGACAACCAGTAGGGCTAGAAGATGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGa                                                                                                       >  1:412429/1‑149 (MQ=255)

                      aCGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTGCGCCAGCAGATGATCGCTGAAATaa                                                                                                  >  2:607197/1‑149 (MQ=255)

                       cGACAACCACTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGGAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATCCTCCGCCAACAGATAATCCCTGAAATaaa                                                                                                 <  1:565716/149‑1 (MQ=255)

                             ccAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAAGCCCTGAAATAAAcgagcg                                                                                           >  2:355832/1‑149 (MQ=255)

                                        aGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCAACCAGCTAATCCCTGAAATAAACGAGCGAGAAGCaagaa                                                                                >  2:569336/1‑149 (MQ=255)

                                           aGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGc                                                                             <  2:399596/149‑1 (MQ=255)

                                             ttGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAGTCCCTGAAATAAACGAGCGAGAAGCAATAATGCCg                                                                           >  1:410220/1‑149 (MQ=255)

                                                tCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCACTAATGCCgggg                                                                        >  1:426934/1‑147 (MQ=255)

                                                    ccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCa                                                                    >  2:289058/1‑149 (MQ=255)

                                                      gATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTGAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACg                                                                  >  1:474805/1‑149 (MQ=255)

                                                                 caTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGtt                                                       >  1:77754/1‑149 (MQ=255)

                                                                   tccTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTc                                                     <  1:623610/147‑1 (MQ=255)

                                                                       gAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATaa                                                 >  2:661105/1‑149 (MQ=255)

                                                                           ggatgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAAGGCCGGAGCCCACGTACCTATAGTTTCATAACCTg                                             <  2:541123/148‑1 (MQ=255)

                                                                            gatgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTgg                                            <  1:426847/149‑1 (MQ=255)

                                                                             atgCGTAAAAACCTTTGCGCCCTTCAACGGCCACTTCCCTCATCTAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTggg                                           <  2:380527/149‑1 (MQ=255)

                                                                                 gTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGgggc                                       >  1:30466/1‑149 (MQ=255)

                                                                                  tAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggca                                      >  1:194041/1‑149 (MQ=255)

                                                                                  tAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGagcca                                      >  1:194532/1‑149 (MQ=255)

                                                                                         cTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATa                               <  2:96348/149‑1 (MQ=255)

                                                                                              cgcCCTTCATCGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg                          <  2:474814/149‑1 (MQ=255)

                                                                                              cgcCCTTCAACGGCAACTTCACTCATGTAGGGGGCGCTGGTGGCATATTCTCCGCCAACAGATAAGCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg                          <  2:606115/149‑1 (MQ=255)

                                                                                                   ttCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATACCCAGCGATc                     >  1:6151/1‑149 (MQ=255)

                                                                                                        cGGCAACTTCACTCATATAGGTGGCGCGGGTCGCATATTCGCCGCCAACAGATAATCCCTTAAATACACGAGCGAGAAGCAATAATGCCGGAGCCCACGCACCTATAGTTGCTTAACCTGGGAGGCAGGCGATAACCAGCGATCCGaaa                <  1:293962/149‑1 (MQ=255)

                                                                                                               ttCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatca         >  2:671929/1‑149 (MQ=255)

                                                                                                               ttCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatca         >  1:236871/1‑149 (MQ=255)

                                                                                                                  aCTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac      >  1:651685/1‑149 (MQ=255)

                                                                                                                    tCATGTAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGAAATAATGCCGGAGACCACGGGCCTGTAGTTTCCTAACCGGGGAGGCAGGCGATGACCAGCGATCCGAAAGACATCATacac    >  2:111248/1‑149 (MQ=255)

                                                                                                                      atatAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCAGAACCCGGGAGGCAGGCGATAACCAGGGATCCGAAACAAACCAAaaacac  >  2:321731/1‑149 (MQ=255)

                                                                                                                      atatAGGTGGCGCTGGTGGCATATTCTCCCCCACCAGATCATCCCGTAAACATACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac  <  2:77754/149‑1 (MQ=255)

                                                                                                                      atatAGGTGGCGCCGGTGGCATATTCTCCGCCAACAGATAATCCCTAAAATAAACGAGCGAGAAGCAATAATACCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGCAACACACCATaccacc  >  2:321727/1‑145 (MQ=255)

TTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACAC  >  CP009273/2718542‑2718808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`