Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,719,426	T→C	intergenic (‑321/+2)	kgtP ← / ← rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,719,426	0	T	C	100.0%	94.3 / NA	36	intergenic (‑321/+2)	kgtP/rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/12);  total (24/12)

ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGC  >  CP009273/2719280‑2719568                                                                                                                                                   |                                                                                                                                               agttgtGGAAGTGGGCAAGTGGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCGATCCGTCAGGATGGCCTTTCGCATAATTCGa                                                                                                                                              <  2:320144/144‑1 (MQ=255)    gagggagctGGGGAAGTTGGGGAAGCGGCATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGGCATCCGTCAGGATGGCCTTTCGCATAATTCGATGc                                                                                                                                           <  2:525466/141‑1 (MQ=25)      tGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTCCGCCAATGCAAAAAGGCCAACCGTCAGGATGGCCTTTTGCCTAATCCGATGCCt                                                                                                                                         >  2:382730/1‑149 (MQ=255)         aaGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGc                                                                                                                                      >  1:328012/1‑149 (MQ=255)         aaGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGc                                                                                                                                      >  1:463864/1‑149 (MQ=255)         aaGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGAGGGAGAAGAAGTCGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTAAGGATGGCCTTTCGCATAATTCGATGCCTGGc                                                                                                                                      >  1:326751/1‑149 (MQ=25)              ggCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTc                                                                                                                                 >  1:242593/1‑149 (MQ=255)                cAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTccc                                                                                                                               >  1:556870/1‑149 (MQ=255)                      ggggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTCCGCCCATGCAAGAAGGCCGTCCGTCAGGATGGCCGTTCGCATAAATCGATACCTGGCAGTTCCCtcccct                                                                                                                         >  2:507798/1‑144 (MQ=255)                        ggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAGAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCCCg                                                                                                                       >  1:401394/1‑149 (MQ=255)                           aGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACtttccgct                                                                                                                    >  1:198256/1‑145 (MQ=255)                               gTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGGCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgggg                                                                                                                >  1:497609/1‑149 (MQ=255)                                  tCCGTTGTCGAATCTGGTATCTGTGGGAGCATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGGAAAAAGGCCATCCGCCCGGATGGCCTTTATCATAATACGATGCCTGGCAGTTCCCTACCTTCGCATGTGgaga                                                                                                             <  2:542370/149‑1 (MQ=18)                                          tGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTTTTTTCTTATGCAAAAAGGCCATCCGTCAGGATGGACTTTCGCATAATTCGCTGCCTGGCAGTTCCCTCCTCTCCCATGGGGCGACCCCacac                                                                                                     >  2:485734/1‑149 (MQ=25)                                           gAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGAATTTTTTGGTGCGCCAATGCAAAAAGGCCATCCGGCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCGACACt                                                                                                    >  1:507331/1‑149 (MQ=25)                                            aaTCTGGCATATGTGGGAGTCTAAGACGGGCGGTGTCGCTTCAGGGATGTTGTTCTGCGCCAAGGCAAAAAGGCCCTCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCGACTCTCGCATGGGGAGACCCCACACTa                                                                                                   <  2:662706/149‑1 (MQ=25)                                                tGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCAt                                                                                               >  1:120938/1‑149 (MQ=255)                                                 ggCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGAGATCCGTCAGGATGGACTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGGCCCCACCCTACCATc                                                                                              >  2:642949/1‑149 (MQ=25)                                                          gggAGTATAAGACGCGCAGCGTCGCATCAGGCTTTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACg                                                                                     <  2:60823/149‑1 (MQ=255)                                                          cggaggaTCACACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCCCATAATTCGATGCCTAGCACTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGTGGCTACg                                                                                     <  2:374987/148‑1 (MQ=21)                                                                  aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                                                             >  2:205809/1‑149 (MQ=255)                                                                      cgcgCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                                                                         >  2:504397/1‑149 (MQ=255)                                                                         gccgCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                      <  2:549982/146‑1 (MQ=255)                                                                               tCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCTCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCgg                                                                <  1:715285/149‑1 (MQ=255)                                                                                           tttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAgg                                                    >  1:219940/1‑149 (MQ=255)                                                                                              ttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACCTCTGAGTTCGGCAGGGGGTCAGgggg                                                 >  2:488114/1‑149 (MQ=255)                                                                                                              aaaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGCGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTAAgg                                 >  2:280284/1‑149 (MQ=255)                                                                                                                aaacggcATCCGTCAGGGTGGCCTTTCGCATAATTCGTTGCCGGGCTGTTCCGTACTCTCGCCTGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgcc                               <  2:265581/143‑1 (MQ=14)                                                                                                                   ggCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccgcc                            >  1:138372/1‑149 (MQ=255)                                                                                                                   ggCCATCCGTCAGGATGGACTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccgcc                            <  1:382730/149‑1 (MQ=255)                                                                                                                     ccatcctgacggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACATCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccgaccg                          >  2:503865/11‑145 (MQ=14)                                                                                                                      cATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCATGTGGGACCACCGCGCTACGGCCGCCAgg                         <  1:393167/149‑1 (MQ=255)                                                                                                                            tCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAtt                   >  1:652730/1‑149 (MQ=255)                                                                                                                              aGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACCACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGAGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCt                 <  1:205809/149‑1 (MQ=255)                                                                                                                                         tCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGGTTTNTCAGa      >  2:689673/1‑149 (MQ=18)                                                                                                                                             aTAATTCGATGCCTGGCAGTTCCATACTCTCGCATGGGGAGACCCCACTCTACCTTCTGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGAGGGACCACCGCGCTACGGCCGCCAGGCAAACTCGGTTTTATCGGACCGc  >  2:504542/1‑149 (MQ=11)                                                                                                                                                   |                                                                                                                                               ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGC  >  CP009273/2719280‑2719568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑