| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 2,861,917 | G→A | Q66* (CAG→TAG) | nlpD ← | activator of AmiC murein hydrolase activity, lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,861,917 | 0 | G | A | 98.0% | 158.4 / NA | 51 | Q66* (CAG→TAG) | nlpD | activator of AmiC murein hydrolase activity, lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (26/23); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCA > CP009273/2861773‑2862060 | gAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCt < 1:581061/149‑1 (MQ=255) aaTGTTCCCAAACTGACAGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTCAGCTACTGGCTGCACTGGCTGGATTTGCGGGTGTTCAGTAGCCTGAATTTGTGGCTGCTGCACCGGCGGAATTTGCGGTTGCTACGCTg > 2:544689/1‑149 (MQ=255) aTGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGt < 1:690328/149‑1 (MQ=255) aCTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGGCgtcgtc > 1:646380/1‑149 (MQ=255) gTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTCGCCACGCGGTGTTCGTCGTCCCCAttt > 1:198900/1‑149 (MQ=255) tatcGACGATGCGTCGGTGTTCCATCTGTCCCGGCTGCTGCGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGACTTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTc < 2:482804/149‑1 (MQ=255) tataGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTc > 1:40733/1‑149 (MQ=255) ataGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTcg < 1:563541/149‑1 (MQ=255) aCGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTcggcgg < 2:430453/149‑1 (MQ=255) ccGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAAt < 1:489885/149‑1 (MQ=255) cGTTTTCCTGCGGTTCCGGCTGCTGAGCGACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATc < 2:381957/149‑1 (MQ=255) cGTTTCCCATCTGTTCCGGCTGCTGAGCTACTGGCCGCACTGGCTGGATTGGCGGTGGGTGAGTCGCCTGAATTTGTGGCTGCTGCACCGGCTGACGTTTCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATc < 2:313159/149‑1 (MQ=255) aTCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATccc > 2:541224/1‑149 (MQ=255) tCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTCCGCTGCAGACGTCGTCCCCCTTTTCGGCGGCGGCGTAATCAACATACCa < 2:616797/149‑1 (MQ=255) tGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGa > 2:418832/1‑149 (MQ=255) ccGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGACGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTa < 2:437249/149‑1 (MQ=255) cGGCTGCTGGGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCGGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTATGCTGTAGACGTCGTCACCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAg < 2:156967/149‑1 (MQ=255) ggCTGCTGAGCTACGGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGt < 1:287732/149‑1 (MQ=255) ctgctgAGCTACTGGCGGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGCATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTAt < 1:581955/149‑1 (MQ=255) gctgAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTAttt > 2:183752/1‑149 (MQ=255) gctgAGCTACTGGCTGCACTGGCTGGATTTGCGGTCGGGGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTAttt > 2:159329/1‑149 (MQ=255) tgAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGc > 1:696071/1‑149 (MQ=255) gAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCa < 1:603644/149‑1 (MQ=255) aGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAg > 1:219622/1‑149 (MQ=255) cTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTTCACGGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGgc > 1:144143/1‑149 (MQ=255) cTACTGGCTGCACTGGCTGGATTTGCGGTAGTTGAGTTGCCTGAATTTGTGGCTGCTGCACCGACTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGgc > 1:478035/1‑149 (MQ=255) tACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGgcg > 1:697242/1‑149 (MQ=255) tGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGCATTAGTATTTGCAGGCGCAt > 1:496258/1‑149 (MQ=255) cTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGc > 1:357828/1‑149 (MQ=255) tGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAc > 1:488132/1‑149 (MQ=255) ggTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAccgg < 1:736053/149‑1 (MQ=255) gttgttGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCGGAccggg > 1:103851/1‑148 (MQ=255) gAGTTGCCTGAATTTGTGGCTGCTGCACCGGGTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCCAGAACATCTCCATTAACGGAGCTGAccggtgccggt < 1:177435/149‑1 (MQ=255) ccTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTGACGGAGCTGACCGGTGCCGGTGGAttt > 1:513899/1‑149 (MQ=255) cTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTg > 1:487186/1‑149 (MQ=255) tGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGa > 1:558693/1‑149 (MQ=255) gAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGaa < 1:39363/149‑1 (MQ=255) tttGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAgtg < 1:183752/149‑1 (MQ=255) gctgctGCACCGGCTGAATTTGCGGTTGCCACGATGCAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTAGTGGCAGGTGCATTGCCATTAACTGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGaa < 2:356786/149‑1 (MQ=255) ctgctgCCCCGGCTGCAGTTGCGGTTGCGACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGTCGTAATCAACAGACCAGAATTAGTATCTGCAGGCGCAGTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAAc < 2:240463/149‑1 (MQ=255) tgctgcACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGGGTCAGAACa > 1:62461/1‑149 (MQ=255) tgctgcACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTGGAAGTGTCAGAACa > 1:62542/1‑149 (MQ=255) tgcACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCCTTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGACGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTCGAAGTGTCCGAACAGcc > 2:329365/1‑149 (MQ=255) gCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGCGCTGCCCGTTTCTTGTGGATTTGAAGTATCAGAACAGCCTgccagc < 1:541827/149‑1 (MQ=255) gCTGAATTGGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCCGACCGGTGCCGGTGGATTTGAAGTGCCACAAccagaggcggtg < 2:7766/149‑12 (MQ=255) tttGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGc > 1:579006/1‑149 (MQ=255) gCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCTGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGaa < 1:17696/149‑1 (MQ=255) ggTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCt < 2:511762/132‑1 (MQ=255) ggTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCt > 1:511762/1‑132 (MQ=255) ttGCTACGCTGTAGCCGTCGTCCCCATTTGCGGGGGCGGCGTACTCAACATACCAGAATTAGTCTTTGCAGGGTCATTGCCATTTGCGGAGCCGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCa < 2:73465/149‑1 (MQ=255) ttGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCCGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGCACAGCCTGCCAGCCATAGCGAAACCa > 1:46761/1‑149 (MQ=255) | GAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCA > CP009273/2861773‑2862060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |