| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 894,431 | Δ1 bp | 13.8% | coding (458/1128 nt) | rlmC → | 23S rRNA m(5)U747 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 894,430 | 0 | A | . | 13.8% | 73.3 / 7.6 | 29 | coding (457/1128 nt) | rlmC | 23S rRNA m(5)U747 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (10/15); new base . (2/2); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
ATCGCCCGAGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAA > CP009273/894286‑894569 | aTCGCCCGAGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGgc > 1:217566/1‑149 (MQ=255) cccGAGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGCACTGCGTTCTGATACCAAGCTGGCGCAACGGCGTAAg > 2:298457/1‑143 (MQ=255) ccGAGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGc < 1:67999/149‑1 (MQ=255) gCGGGGTTAACGCCCTACAACGGGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATCATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCt < 2:191424/143‑1 (MQ=255) aCGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGT‑AGGCGCTGCCGTGGTTACACGaa > 1:105180/1‑149 (MQ=255) aCGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGT‑AGGCGCTGCCGTGGTTACACGaa > 1:105019/1‑149 (MQ=255) gCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCGGCCGTGGttgc > 2:272550/1‑141 (MQ=255) gCCCGACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGaac < 1:287714/149‑1 (MQ=255) cccTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTaca < 2:24591/143‑1 (MQ=255) aCAACGTGGCGCGTAAGCGTGGCGAACTGCAATACATTCTGCTGACTGAAAGCCAGAGGGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTGCACgga > 2:210471/1‑141 (MQ=255) cGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGc < 1:200624/149‑1 (MQ=255) tGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAg < 1:62442/149‑1 (MQ=255) gactgcACGCCAGAGTGACGGAGGCATTATGCTGCGCTGTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCgg < 2:281182/143‑1 (MQ=255) gactgaAAGCCAGAGTGATGGAGGCATGATGCGGCGCTTTTTACGGCGTTCTGATACCAAGCTGGCGCATCTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCgg < 2:129410/143‑1 (MQ=255) actgaAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACatat > 1:191972/1‑149 (MQ=255) tgaAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATgg > 1:322672/1‑149 (MQ=255) ccAGAGGTATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATgg < 2:198389/143‑1 (MQ=255) cAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAGAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTAt > 1:256013/1‑149 (MQ=255) cAGAGTGAGGGAGGCATGATGCTGTAGTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGATACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTAt < 1:201261/149‑1 (MQ=255) gggatgatgCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCCGCCGGTACATATGGCGATTATGGAAgg < 2:172357/140‑1 (MQ=255) gcaggctgctgcGCTTTGGTCGGCGTTCTAGTACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCGGAAAGTTATGACCGTCAATATTGAGCCGGTACATATGGCGATTATGGAAggg < 2:125845/137‑1 (MQ=255) ttttGTACTCGCTTCGGATACCAAGCTGGCGTAACTGCGTAAGTCGCTGCCGTGCTTACACGAACAACTCTCGCACCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCCATTATGGAAGGGTAGACGGAGATCt < 2:23068/142‑1 (MQ=255) cTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCt < 2:172527/143‑1 (MQ=255) ttCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGTAGGGGCGACGGGGATCTCCCTGACCGaac > 2:265670/1‑143 (MQ=255) gATACCAAGCTGGCGCAACTGCGT‑AGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTACCTGACCGAACAACAg < 2:92718/143‑1 (MQ=255) gATACCAAGCTGGCGCAACTGCGT‑AGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGAGCTACCTGACCGAACAACAg < 2:92858/143‑1 (MQ=255) gtACCAAGCTGGCGCAACTGCGTAAGGCGCGGCCGTGGTTACGCGAACAACTACCGCAGCTGAAAGTTATTACGGTCAAGATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGACCTACCTGACCGAACAACAg > 2:263228/2‑143 (MQ=255) tACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTACCTGACCGAACAACAgg < 2:290884/143‑1 (MQ=255) cGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTACCTGACGGAACAACAGGCACTGGGGGGGGGTTTTaa > 2:52740/1‑143 (MQ=255) | ATCGCCCGAGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATGGAGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAACAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAA > CP009273/894286‑894569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |