Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 63.6% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)5 (0.280) 5/270 NT 44.7% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 6 (0.340)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)16 (0.890) 13/270 NT 72.1% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 6 (0.340)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

TTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTT  >  CP009273/2282254‑2282524
                                                                                                                                                |                                                                                                                               
ttCTTGTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTACCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTCAATATaaggaag                                                                                                                             <  1:238577/149‑1 (MQ=255)
  cTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaag                                                                                                                                 <  2:235719/143‑1 (MQ=255)
          ttcTGTTCTCCGCCAGGATTGATTTTCGTATTACTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACGCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGc                                                                                                                         <  2:44878/141‑1 (MQ=255)
                 ctcCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGa                                                                                                            >  1:168564/1‑149 (MQ=255)
                            ttgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGTAAAttc                                                                                                       >  2:213471/1‑143 (MQ=255)
                                                 gTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTcatttcattt                                                                            >  1:296849/1‑149 (MQ=255)
                                                    ccATAAACCAGCTGGATATCTTTGTCCAGGACAACCCCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATCTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                         <  1:57623/149‑1 (MQ=255)
                                                                       cTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                                                      >  1:304138/1‑149 (MQ=255)
                                                                        tttttCCAGGACAACACCATCAACTGTCTACCCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGCAATTCTTCTCGGCTGACTCAGTCCTTTCATTTCTTGATATTTGATCCGa                                                           >  2:172160/1‑143 (MQ=25)
                                                                           ttCCAGGACAACCCCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                  <  1:183974/149‑1 (MQ=255)
                                                                                  acaacaCCACCAACTGTCCCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                           <  1:114166/149‑1 (MQ=255)
                                                                                    aacaCCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCGGTCATTTCCTTTCTTCACGTTTGAGCCGATTCTTTCTCCCg                                               >  2:10543/1‑143 (MQ=21)
                                                                                             aagcgTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCt                                      <  2:205230/139‑1 (MQ=255)
                                                                                                    tcACCATACGAAACGGTAGATGCCAATGCTATGGGTGAATATAAGCAAGGTGCGAATAAGCGGGGAAATTCTTCTCCGCTGATTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGGAAATGCCTTGAATCa                              <  2:31235/143‑1 (MQ=17)
                                                                                                             tCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt               >  1:251658/1‑149 (MQ=255)
                                                                                                                  aGTTGCCGCCAATGTTATGGGTGAATATAATGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttctt          <  1:239395/149‑1 (MQ=255)
                                                                                                                                cTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCCTTTCATTTCTTGATGTTTGAGCCGATTTTTTATTCCTTAATGTCTTTTATACATCCTATTTAGACCGTTTCTTCGCCCttt  >  2:133213/1‑143 (MQ=14)
                                                                                                                                                |                                                                                                                               
TTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTT  >  CP009273/2282254‑2282524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.