| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,089,622 | A→G | 11.5% | W278R (TGG→CGG) | rhaT ← | L‑rhamnose:proton symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,089,622 | 0 | A | G | 11.5% | 73.6 / 4.4 | 35 | W278R (TGG→CGG) | rhaT | L‑rhamnose:proton symporter |
| Reads supporting (aligned to +/- strand): ref base A (17/14); new base G (2/2); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01 | |||||||||||
TCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCACTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGCCAGACGAATAAAACAGAAACCGAG > CP009273/4089481‑4089769 | tCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCACTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTTCGCCGGAATGCGGGCGTGGCCCCAg < 2:162963/143‑1 (MQ=255) cacCGGTACCGGCCGGCGTCCTGCATTGTGCCACTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGaaaa < 1:83199/149‑1 (MQ=255) acCGTGACCGGACGGCGTCCGGCATTGTTCCACTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATa < 2:258428/143‑1 (MQ=255) cGTTACCGGTCGGCGTCCTGCATTGTTCCACTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGACACTCATATGCAGCATCCAACTGATGTAGTCAGACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGa < 1:160653/149‑1 (MQ=255) ccGGACGGCGTCCTGCATTGTTCCACTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAg < 2:125221/143‑1 (MQ=255) cGGCGTCCTTCATTGTTCCACTCTTGCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCTTAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTg < 2:273216/143‑1 (MQ=255) tGCATTGTTCCACTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAGACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAgttcacacctca > 1:65458/1‑138 (MQ=255) ccACTCTTTCAGCACCAGCCCGACGTTCCCGCCGCACAATACATAGAAACGCATATGCGGCATCCAACTTATGTAGTCGTACTGAGCCGGAAGGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAAcc > 2:211526/1‑143 (MQ=255) aCTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAgt > 1:110203/1‑149 (MQ=255) cACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAAGTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATGCCACATCACACCGCCCAggggcg > 2:187841/1‑138 (MQ=255) aCCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGGGAGaaa > 1:151390/1‑149 (MQ=255) aGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCGGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCgaggg > 2:138822/1‑141 (MQ=255) aGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCGGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGagag > 2:56790/1‑143 (MQ=255) cgaTACCGCCGCACAACACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAAcaca < 2:20698/143‑1 (MQ=255) aCCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGa < 1:268133/149‑1 (MQ=255) ccgcACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAg < 1:69621/149‑1 (MQ=255) ccgcACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAg < 1:69717/149‑1 (MQ=255) aTAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCa > 1:261822/1‑149 (MQ=255) aGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGACCCCAGACATAGAAAAAGAATTGCAGATACCCCACCAACCCGCCCAGTGTCGCGAGTAACACATTGTGAATGATCAGGGAGTTTGcaacc > 1:205294/1‑145 (MQ=255) atatGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATGGTGAATGATCAGCGATTTTGCCAGCGAGAAggc > 1:141144/1‑147 (MQ=255) caTCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCgg < 2:261255/143‑1 (MQ=255) aTCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGGAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCttttcg > 1:73037/1‑147 (MQ=255) tCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCt < 2:136796/143‑1 (MQ=255) caGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCtt < 1:97905/148‑1 (MQ=255) tCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGGGTCGAGAGTACCACATTGTGAGTGATCAGCGATTTGGCCAGCGAGCAGTCGGCTTTTAGCGACAAATc > 2:105198/1‑143 (MQ=255) tACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGAGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCTTCACttt > 1:212523/1‑149 (MQ=255) tGCGCCGGAATGCGGGCGTGGCCCCGGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTTAATGATCAGCGATTTTGCCAGCGCGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGc < 1:63729/149‑1 (MQ=255) tGCGCCGGAATGCGGGCGTGGCCCCGGGCATAGAAAAAGAATTGCAGAACCAACCGCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGc < 1:158451/149‑1 (MQ=255) ccGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGCCAGa > 1:122309/1‑149 (MQ=255) gtaaTGCGGGCGTGGCCCCAGGCATAGAGAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGGGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGGCGGCTTTTAGCGACAAAACCTTCACTTTTGc > 2:2384/3‑143 (MQ=255) gCGGGCGTGGCCCCAGGCATCGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTATGCCAGCGAGAAGTCGGCTTTTGGCGACAAATCCTTCACTTTGGCCGGAc > 2:210841/1‑143 (MQ=255) gggCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGCCAGACGAATaaaa > 1:237840/1‑149 (MQ=255) gCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGCAAGACGAAGacaaca > 1:245975/1‑149 (MQ=255) cccAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGCCAGACGAATAAAACAGAAACCg < 1:91559/149‑1 (MQ=255) cAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGTGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGCCAGACGAATAAAACAGAAACCGAg > 1:242235/1‑149 (MQ=255) | TCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCACTCTTTCAGCACCAGCCCGACGATACCGCCGCACAATACATAGAAACTCATATGCAGCATCCAACTGATGTAGTCATACTGCGCCGGAATGCGGGCGTGGCCCCAGGCATAGAAAAAGAATTGCAGATACCACATCAACCCGCCCAGTGTCGAGAGTAACACATTGTGAATGATCAGCGATTTTGCCAGCGAGAAGTCGGCTTTTAGCGACAAATCCTTCACTTTTGCCAGACGAATAAAACAGAAACCGAG > CP009273/4089481‑4089769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |