| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,909,455 | G→A | 38.8% | Q113* (CAG→TAG) | proQ ← | RNA chaperone, putative ProP translation regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,909,455 | 0 | G | A | 38.8% | 18.2 / 13.7 | 23 | Q113* (CAG→TAG) | proQ | RNA chaperone, putative ProP translation regulator |
| Reads supporting (aligned to +/- strand): ref base G (6/8); new base A (3/6); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAG > CP009273/1909313‑1909598 | ttttGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg > 1:231355/1‑149 (MQ=255) cggggTTTCCGTTCAGCGGCGTCTTTGCGGGGTGGCGTCGTCGGCCGTGGCTTGCGCTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCCTGCTGTTCAGCACGCTATGCCTGAac < 2:80909/140‑1 (MQ=255) gTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc > 2:25522/1‑143 (MQ=255) tcttTGGGGCGGGGCGTAGTCGGGCGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCCGCAGTTGCGGCAGCTTCGCGTTGTTTCGCTTGCGTTTCAGCACGCTATGCCTGAACACGCGCTTTCGCTTCTTCAAg < 2:140141/143‑1 (MQ=255) cgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTATGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCg > 2:228942/1‑143 (MQ=255) tCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGctct > 2:251970/1‑143 (MQ=255) gTGGCTTGCGTTTGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTTTTCAGCCCGCTATGCCTGCACACGCGCTTTCGCTTCTCCAAGCTGCTTGCGAGCATGCTCTACATGt < 2:174479/143‑1 (MQ=255) ttCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCAc > 1:9235/1‑149 (MQ=255) gCGTCTTCTTTCTCACCAGCAGTTGCGGCCGCTTCGCGTTTTTTCGCTTGCTGTTCAACACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGcc < 1:291774/149‑1 (MQ=255) cttcttTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTATGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTg < 2:168222/143‑1 (MQ=255) tttctCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGt < 2:36823/143‑1 (MQ=255) ttctCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTGGCCGTc > 2:123124/1‑143 (MQ=255) ctcACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGa < 1:316329/149‑1 (MQ=255) cagcggTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCg < 2:140295/143‑1 (MQ=255) aggtGCGTCCGCTTCGCTTTTTTTCGCTTGCTGTTCAGCACGCTGTTCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCCAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTg < 1:10835/146‑1 (MQ=255) ttCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCgg < 2:102207/143‑1 (MQ=255) tCGCGTTTTTTTGCTTGCTGTTCAGCACGCTGTGCCTGAGCACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTTGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAac < 1:28841/149‑1 (MQ=255) tCGCGTTTTTTCGCTTGCTGGTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAac < 1:28882/149‑1 (MQ=255) tCGCTTGCTGTTCAGCGCGCTATGCCTGAACACGCGCCTTCGCTTCTTCACACTGCTTGCGCGCATGCTCTACATGTTGCATGTCGAGCTCCCGGCATGGGGTTCCGTCAAGGTCGACACGCGTTGCGCCGGGTTTAAGACCg > 2:125537/1‑143 (MQ=255) ttCAGCACGCTATGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGc > 1:128795/1‑149 (MQ=255) aCGCTATGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGc < 2:218383/143‑1 (MQ=255) aCGCTATGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAg < 1:149580/149‑1 (MQ=255) gCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTc > 2:132234/1‑143 (MQ=255) | TTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAG > CP009273/1909313‑1909598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |