Predicted mutation
evidence seq id position mutation annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)32 (0.490) 22/292 3.2 100% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 0 (0.000)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
Rejected: Coverage evenness skew score above cutoff.
* ? CP009273 = 2283592NA (NA)102 (1.550) 65/292 0.0 100% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 0 (0.000)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/2282535‑2282398
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTT  >  CP009273/2400257‑2400403
                                                                                                                                                                                                                                                                                             
GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAGATGAAATGACTCAATCAGCCGAGAAGACTTTCCCCGCTGATTCGCACCTTCCCTAcag                                                                                                                                               >  2:446223/1‑141
             ATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTC                                                                                                                             <  1:476677/149‑1
              TGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCC                                                                                                                            <  1:393739/149‑1
                  GAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGCAGAAATGAAGTGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTA                                                                                                                        >  1:239036/1‑149
                  GAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTCCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTA                                                                                                                        >  1:429638/1‑149
                   AAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAACAATAGGCTCAAACCTGAAGAAATGAAATGGCGGAGGCAGTCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAACGACTCACATTGTTCCGTTAT                                                                                                                       >  1:777825/1‑149
                   AAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCGTATTCGCACCTTCCCTAAAAAAGCGCCTCACATTGTTCCGTTgt                                                                                                                       >  1:663926/1‑147
                                          TTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAA                                                                                                <  1:752771/149‑1
                                          TTCAAGGCATGTACGGGAGAAAAAATCGGCTCAAACATGAATAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAA                                                                                                <  1:177926/149‑1
                                           TCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTCCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCAC                                                                                                    <  2:328698/144‑1
                                            aaacGCAGTTACGGGAGAAAAAAGCGGCTCAAACATCAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTCCCCCGCTTATTCGCACCTCCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATt                                                                                              <  1:21930/145‑2
                                             AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGGTCACAGAATAT                                                                                             >  1:54453/1‑149
                                                   TTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATA                                                                                            >  2:63236/1‑144
                                                                                 AAGAAATGAAATGACTGAGTCAGCCGAGAAGACTTTCCCCGCTTATTCGCAACTTCCCTAAAAAAGCGACTCACATTGTTCCGTTAAAATGCCTGAAGGAGATCACAGAATCTATCTTCAGGAAGCGCATATCTATTACGTTAC                                                              >  2:963257/1‑144
                                                                                 AAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCCCATTGTTCCGTTATAATGCCTGAAGTAGATGACAGAATATAACTTCAGGGATCGCCTATCTATTAAGTTACTCcgt                                                         >  1:88726/1‑146
                                                                                 AAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACT                                                         >  1:11366/1‑149
                                                                                   GAAATGAAATGACTGAGTCAGCCGAGAAGAATTGCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCGGGGATAGCACATCTATTAAATTACTCACcgt                                                       >  1:6746/1‑146
                                                                                    AAATGAAATGACGGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCA                                                           <  2:504662/144‑1
                                                                                               CTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTAT                                                <  2:598586/144‑1
                                                                                                TGAGTCAGCCGAGAAGAATTTCCCCGCTTACTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCAAAGCTATTAAGTTAGTCACTCTTTTCTATTTATGA                                          >  1:585007/1‑149
                                                                                                  AGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTA                                             >  2:289473/1‑144
                                                                                                   GTCAGCCGGGAAGAATTTTCCCTTTTGATGGCACCTGCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTCTTCACTCTTTGCTATTTAT                                            <  2:459415/144‑1
                                                                                                   GTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTAT                                            <  2:292765/144‑1
                                                                                                      AGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACTTGCG                                    >  1:228498/1‑149
                                                                                                       GCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGAGATGCGC                                   >  1:176022/1‑149
                                                                                                               GAAGTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGCCTCACATTGTTCCGTTATAGTGCCTGAAGTCGATCACACAATATATTTCCAGGGATCGCATATCTATTACGTTACTCACTCCTTTATATTTTTGACATGCGgntc                                >  2:945551/1‑140
                                                                                                                  TTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATATTATTGTTTTTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATA                        <  1:1098127/149‑1
                                                                                                                  TTTCCCCGCTTATTCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGAGATcggaagagcg                             >  2:523265/1‑134
                                                                                                                               TCGCACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAA                <  2:562921/144‑1
                                                                                                                                  CACCTTCCCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGT             <  2:386285/144‑1
                                                                                                                                        CCCTAAAAAAGTGGCTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTT  <  1:116978/149‑1
                                                                                                                                        CCCTAAAAAAGCGACTCACATTTTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCGTATCTATTAAGTTACTCACTCTTTTGTATTTATGACATTTTCTGTTTTGTATAAATGTATTTGTGAGTCCTTGTT  <  1:827183/149‑1
                                                                                                                                                                                                                                                                                             
GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/2282535‑2282398
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTT  >  CP009273/2400257‑2400403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.