Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,719,426	T→C	intergenic (‑321/+2)	kgtP ← / ← rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,719,426	0	T	C	100.0%	117.7 / NA	39	intergenic (‑321/+2)	kgtP/rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/15);  total (24/15)

TGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719285‑2719566                                                                                                                                              |                                                                                                                                             tGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAAGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCCATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGa                                                                                                                                            >  1:59702/1‑144 (MQ=255)   gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATg                                                                                                                                          <  1:41114/144‑1 (MQ=255)   gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATg                                                                                                                                          >  1:423630/1‑144 (MQ=255)        ggtcAAGTGGGGGAGGCCGCATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTgg                                                                                                                                     <  1:187718/141‑1 (MQ=25)                    gAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAACAAGCCATCCGCCAGGATGGCCCTTCGCCTAATTCGACGCATggacactccccacg                                                                                                                         >  1:72498/1‑132 (MQ=21)                     aaGCCGTATCCGTTGCTGAATCTGGCATATGTGGGCGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCa                                                                                                                   >  2:621567/1‑149 (MQ=255)                        ccGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                                <  2:297847/149‑1 (MQ=255)                           tATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCCGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCCAAAAGGCCATCCGTCAGGATGGCCTTTCGCCTCATTCGATGCCTGGCAGTTCCCTACTCTCTCATGGGga                                                                                                             >  2:97687/1‑149 (MQ=25)                             tCCGTTGGTGGATGTGGCGTATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                                <  1:153583/144‑1 (MQ=21)                                  tGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCca                                                                                                      <  2:439127/149‑1 (MQ=255)                                       aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa                                                                                                 >  2:735520/1‑149 (MQ=255)                                         tCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                    <  1:658420/144‑1 (MQ=255)                                                       gAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATCATTCGCTGCCTGGCAGTGCCCTACTCTCGCATGGGGAGACCCCACACTACAAGCGGCGCt                                                                                      >  1:85021/1‑144 (MQ=25)                                                        aGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTTTTTTTTTTTTCCAAGAAGCCATCCGTCAGGATCGCCTTTCGCGTAGTTCGATGCCTCGCAGTTCCCTCCTCTCGCATGAGCAGCCCCCCCACTCCCCTCCgcgcca                                                                                     >  1:275249/1‑142 (MQ=255)                                                          tataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGGCATCCGTCAGGATGGCCTTTCGCCTAATTCGATGCCTGGCAGTTCCCAACTCTCGCATTGGGAGGCACCACACTTCCAACGGCGCGACGGCGtt                                                                              >  2:204331/1‑149 (MQ=18)                                                            taAGACGCGCAGCGTCGCATCAGGCATTTTTTTTTTTTTTTTTGCAAAAAGGCCATCCGACAGGATTGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGGGACCCCACACTCCCACCGGCCCTCCgcc                                                                                 >  1:174218/1‑142 (MQ=21)                                                                  gcgcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCt                                                                      >  2:123755/1‑149 (MQ=255)                                                                   cggaGCGTCGTATCAGGCTTTTTTTTCTGCGCCAATGCAAAATGGCTATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTg                                                                     <  2:217905/146‑1 (MQ=21)                                                                   cgcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTg                                                                     >  2:414938/1‑149 (MQ=255)                                                                    gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTACGGCGTTTCACTCCTgc                                                                    >  2:672691/1‑148 (MQ=255)                                                                     cAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAg                                                                   >  2:680931/1‑149 (MQ=255)                                                                          tCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCgg                                                              <  2:434495/149‑1 (MQ=255)                                                                             cATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAt                                                           >  2:288422/1‑149 (MQ=255)                                                                                       ttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                                                 <  2:200042/149‑1 (MQ=255)                                                                                                 gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATCATTCGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg                                       >  2:533970/1‑149 (MQ=255)                                                                                                  ccAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                      >  2:162526/1‑149 (MQ=255)                                                                                                  ccAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                      <  2:220490/149‑1 (MQ=255)                                                                                                  ccAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                      <  2:586509/149‑1 (MQ=255)                                                                                                          aaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgc                              >  2:365760/1‑149 (MQ=255)                                                                                                                ccATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                        >  2:34841/1‑149 (MQ=255)                                                                                                                 cATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccg                            >  1:412518/1‑144 (MQ=255)                                                                                                                 cATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAgg                       >  2:630470/1‑149 (MQ=255)                                                                                                                  aTCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGc                      >  2:536169/1‑149 (MQ=255)                                                                                                                     cGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                        <  1:84514/144‑1 (MQ=255)                                                                                                                             tGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGttt           <  2:520850/149‑1 (MQ=255)                                                                                                                              ggCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGGCAGGCAAATTCt               >  1:302333/1‑144 (MQ=255)                                                                                                                              ggCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGtttt          <  2:565795/149‑1 (MQ=38)                                                                                                                                ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        <  2:468107/149‑1 (MQ=37)                                                                                                                                      gCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCCCCGCGCTACAGCCGCCAGGCAAATTCTGTTTTATCAGAcc  >  2:611463/1‑149 (MQ=12)                                                                                                                                              |                                                                                                                                             TGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719285‑2719566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑