| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,180,810 | G→A | 16.8% | pseudogene (31/2445 nt) | yqiG → | pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,180,810 | 0 | G | A | 16.8% | 46.7 / 3.7 | 25 | pseudogene (31/2445 nt) | yqiG | pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (6/14); new base A (2/2); total (8/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
ATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCC > CP009273/3180668‑3180954 | acaaCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATg < 1:92638/147‑1 (MQ=255) tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg < 2:234205/149‑1 (MQ=255) ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGCTTTAAGCGCAGc > 1:187194/1‑149 (MQ=255) gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAACTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAg > 1:287056/1‑149 (MQ=255) gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAACTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAg > 1:287058/1‑149 (MQ=255) aCGGGAATATCTGCGGCAGCGCGCTTGTAGTGGTTTAAGTTGTAACAGATTTCTGGAAATATAGGGGCAAATACAGTTATCATCATTGGTTGTGCGTCGGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAGTATCGGGa > 1:229796/1‑149 (MQ=25) ttacattatCGGCGGCAGCTGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTAAACATAGATTTAATCGAAGCCGAAGATCGTGaa < 2:295465/143‑1 (MQ=25) cGGCCGGGGGCTTGTAAGTGTTTAAGTGGAGACAGGTGTCTGGAAATATAGGGGCAACCCCAGGTAGCATCATGTCTTTTGCTGCACCTGATGCCGTTGAATTCCACAAATAGTTAATCGAATCCGAAGATCGTGAAACCGTTAAccct < 1:86905/149‑3 (MQ=255) gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTc > 1:293390/1‑149 (MQ=255) cAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTcc > 2:254280/1‑149 (MQ=255) ttGTGATGGGTTAAGTGAGAACAGATGGCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTAGGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGa < 1:106945/149‑1 (MQ=255) gTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGaaa < 1:226347/149‑1 (MQ=255) gggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCCTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGAt > 2:58006/1‑149 (MQ=255) gTTAAGTGATAACGGATGGCGGGAAATATAGGGGCAATGCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATgg < 1:45129/149‑1 (MQ=255) agggATAACGGCTGTCTGGAAATACAGGGGCAAATCCAGTCATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTACTCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCaa < 1:31038/146‑1 (MQ=255) ataGGGGCAAATCCAGGTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTGTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACt < 1:143936/149‑1 (MQ=255) aaTCCAGTTAGCATCAGTGGTTGGGCGTCAGCCTCTGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAGCTGATGGCCAATTACCCGTCGGAAAATAATCGCTACGCActct < 1:37541/149‑1 (MQ=255) ccAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGAt < 2:187194/149‑1 (MQ=255) tATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaata < 2:210669/149‑1 (MQ=255) catTGGTTGTGCGTCAGCTTATGCAGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAACACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTACGCACTCTGATTAATAATAaga < 1:198015/149‑1 (MQ=255) tGGTTGTGCGTCAACTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAACCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGa < 2:287058/149‑1 (MQ=255) tGGTTGTGCGTCAACTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGa < 2:287056/149‑1 (MQ=255) ttGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGc < 1:254280/149‑1 (MQ=255) cGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATc > 2:114872/1‑149 (MQ=255) gTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATTGTGTAAACGTTAACCTTTCCCAATTGTAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTATTAATAAGAGGACGCCAATcc < 1:108855/149‑1 (MQ=255) | ATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCC > CP009273/3180668‑3180954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |