| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,268,411 | C→T | 17.4% | A150V (GCC→GTC) | chaC → | cation transport regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,268,411 | 0 | C | T | 17.4% | 87.3 / 8.1 | 42 | A150V (GCC→GTC) | chaC | cation transport regulator |
| Reads supporting (aligned to +/- strand): ref base C (17/17); new base T (3/4); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACT > CP009273/1268265‑1268558 | cGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCa < 2:118821/149‑1 (MQ=255) cccacAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGtt < 2:266347/148‑1 (MQ=255) accacAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTACACGCCATTGTGtt > 2:4717/1‑149 (MQ=255) acaGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTttat > 1:165489/1‑149 (MQ=255) tCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGAc > 1:54489/1‑149 (MQ=255) gCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTAGTATGGACCCGCGACATCCAg > 1:218717/1‑149 (MQ=255) cAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAACCGCCATTGTGTTTGTTATGGACCCGCGACGACCAgga > 2:108307/1‑147 (MQ=255) ggagagACGCTGGAGCAGGAACTACCCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAata < 2:244150/147‑1 (MQ=255) gaagcGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAata < 2:79549/149‑1 (MQ=255) gCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTg > 2:88469/1‑149 (MQ=255) gCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTg < 2:137754/149‑1 (MQ=255) gCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCAAAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTg < 1:109526/149‑1 (MQ=255) cTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTAACTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGGCGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGa > 2:275283/1‑149 (MQ=255) gCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTc > 2:234433/1‑149 (MQ=255) cTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACTCTAAACGCCAGCGTGTTCATTTCGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCa < 2:277548/149‑1 (MQ=255) cTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTACGCGGCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCa < 2:277557/149‑1 (MQ=255) cccTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGTCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTc > 2:177757/1‑149 (MQ=255) cccTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGTCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTc > 2:177755/1‑149 (MQ=255) cccTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGTCATTGTGTTTATGATGGACCCGCGACATCCAGAAGAGGAATCTGATACTCGCGCTCAGGTc > 2:177756/1‑149 (MQ=255) cccTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTc > 2:29828/1‑149 (MQ=255) cccTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTc > 2:29811/1‑149 (MQ=255) gtgGAAGCGACAGATGATTACCGTCTGTTATCCGCCAACCTGGTGTCAGCTGGATCGTGATGATGGACGCACAGTAACCGCCATTGTGTTTATGATCGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCgcgc < 2:27523/149‑1 (MQ=255) tgGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGcc < 1:251798/149‑1 (MQ=255) gAACCGAGAGACGATTACCGGCTGTTATCCGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGt < 1:263716/149‑1 (MQ=255) gatTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTgcggcgg > 2:183687/1‑149 (MQ=255) ttATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTTCGGCGGCGAGCGGTCCGc > 2:126842/1‑149 (MQ=255) tCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAACCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTgg < 1:234433/149‑1 (MQ=255) ccgCCAACCTGTTTTCCGCTTGATCTTGATGATGGACGCACAGTAAACGTCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTggg < 1:177756/147‑1 (MQ=255) cTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGTCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTggg < 1:177757/149‑1 (MQ=255) cTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGTCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCAGCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTggg < 1:177755/149‑1 (MQ=255) cTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTggg < 1:29828/149‑1 (MQ=255) cTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTggg < 1:29811/149‑1 (MQ=255) tGCCAACCTGGGGTCAGCTTGATCTTGATGATGGGCGCACAGTAAACGTCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGa < 1:95249/149‑1 (MQ=255) gCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATCTGAATCTGATACTCGCGCTCAGGTCCTCGCCCCGTTGATTGCGGCGGCGAGCGGCCCGCTgtgcc > 2:256868/1‑145 (MQ=255) gCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCACAATAGGAATCTGACACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCTCTGGGaa < 1:4717/149‑1 (MQ=255) cTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGCACCAACg > 1:9390/1‑149 (MQ=255) cttgatGATGGACGCAAAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTc > 2:45930/1‑149 (MQ=255) tgatgaTGGCCGGCCAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCAc < 1:108307/149‑1 (MQ=255) tgatgaTGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACTATACCTGTTTTCAc < 1:275283/149‑1 (MQ=255) cacaGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATGGCGCCGTTGATTGCGGCTGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGc > 1:446/1‑149 (MQ=255) aCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCCTATCaaa > 1:47580/1‑149 (MQ=255) gCCATTGTGTTCAATATGGACCCGCGACATCCAGAATAGGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACt < 1:49382/149‑1 (MQ=255) | CGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACT > CP009273/1268265‑1268558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |