Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	CP009273	542,271	IS5 (–) +4 bp	coding (136‑139/1668 nt)	fdrA →	putative NAD(P)‑binding acyl‑CoA synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	269666 =	NA (NA)	25 (0.590)	23/296	1.0	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	CP009273	= 542274	0 (0.000)					coding (139/1668 nt)	fdrA	putative NAD(P)‑binding acyl‑CoA synthetase
*	?	CP009273	= 270860	NA (NA)	62 (1.460)	47/296	0.0	100%	noncoding (320/1221 nt)	IS30	repeat region
	?	CP009273	542271 =	0 (0.000)					coding (136/1668 nt)	fdrA	putative NAD(P)‑binding acyl‑CoA synthetase

ACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/269799‑269666 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAGGCGTGGATCATAACAACT  <  CP009273/542274‑542129                                                                                                                                                                                                                                                                                          cCGCCTTAAATGGCGAAGAAACGGCCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTAT                                                                                                                                     <  2:642350/148‑1               GAAGAAACGGTCTAGATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCGCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCC                                                                                                                       >  2:427773/1‑149                       GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTAC                                                                                                               >  1:308418/1‑149                           TAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTAGCCATCATTACGGga                                                                                                           >  1:55707/1‑147                             aacAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAAC                                                                                                         <  2:11748/146‑1                                          AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTT                                                                                            >  1:252469/1‑149                                           AGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTT                                                                                           >  1:397275/1‑149                                                        AGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCACGCTTATTCGCACCTTCCCTAATAAAGCTTTCTTGGCGGGCGTACCCATCCTTACGGAAAAAACATCAACAATTTCTGATTCGCAGAG                                                                              >  2:313186/1‑149                                                               ATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACACCATCAACATTTTCTGATTCGCTGAGTTCTCGT                                                                       >  2:339452/1‑149                                                                                      AAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATTATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGA                                                >  2:37132/1‑149                                                                                       AATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACCCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTATGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAA                                               >  2:450634/1‑149                                                                                            CTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGTCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTTAGTTTTCGTGAAATCATCATTAAACTGACCGAAGCCTG                                          >  2:206374/1‑149                                                                                             TGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGCAATAATCATTAAACTGACCGAATCCTGA                                         >  1:258632/1‑149                                                                                                   AGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCATAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACAC                                   >  1:340396/1‑149                                                                                                     CCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCC                                 >  2:681403/1‑149                                                                                                     CCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCC                                 >  2:680902/1‑149                                                                                                        AGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACACCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTT                              >  1:277330/1‑149                                                                                                            GAATTTCCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACAGCATCAACATTGTCTGAATCGCTGAGTTCTCGGGAAATAAACATTAAACTGACCGAATCCTGGAAACACCCTTTTTTA                          >  2:351430/1‑149                                                                                                                  CCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAG                    <  1:616821/149‑1                                                                                                                  CCCCGCTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAG                    <  1:110052/149‑1                                                                                                                       CTTATTCGCACCTTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAGGCGTG               <  2:282743/149‑1                                                                                                                             CGCCCCTGCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCATCATTTTCTGATTCGCTTAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAGGCGTGGATCAT         <  2:308631/149‑1                                                                                                                              GCACCTTCCCTAATAACGCTTTATTCGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAGGCGGGGATCATA        >  1:348668/1‑149                                                                                                                                   TTCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAGGCGTGGATCATAACAAC   >  1:520661/1‑149                                                                                                                                    TCCCTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCCTCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAGGCGTGGATCATAACAACT  >  1:654400/1‑149                                                                                                                                                                                                                                                                                          ACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/269799‑269666 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAATAACGCTTTATTGGCGGGCGTACCCATCATTACGGAAACATCATCAACATTTTCTGATTCGCTGAGTTTTCGTGAAATAATCATTAAACTGACCGAATCCTGAAAACACCCTTTTTTAATAAAGGCGTGGATCATAACAACT  <  CP009273/542274‑542129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.