Predicted mutation
evidence seq id position mutation annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)48 (1.130) 33/296 0.3 100% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 0 (0.000)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)35 (0.820) 29/296 0.5 100% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 0 (0.000)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2283453‑2283592
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAGTGATAATATAT  <  CP009273/2400260‑2400119
                                                                                                                                                                                                                                                                                          
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAA                                                                                                                                       >  2:384786/1‑149
       TCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATAT                                                                                                                                <  1:231453/149‑1
         TTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGC                                                                                                                              >  1:198519/1‑149
             acGGTCTGACGGCGCTAACTGCTGAATTCCCTCGCGGCGAAGGCAAGTGGATGCCTCATGGTGACCCCTGTTCTATGGCTCCAGATGACAAACATCATCTCATATCAGGCACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTT                                                                                                                          <  2:363261/147‑1
                GTCGGACGGCGCTTCCTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTA                                                                                                                       <  2:624/149‑1
                     ACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTC                                                                                                                  >  1:188504/1‑149
                                TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCC                                                                                                       >  1:184747/1‑149
                                  CTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGCCTTGTTCGCACCTTACTTAGTGCAACTCATATGCGTTTTTACTTTCGTCCGGGTTCCGG                                                                                                     >  2:36772/1‑149
                                    GAATTCACTGTCGGTGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGAT                                                                                                   <  2:252388/149‑1
                                    GAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCCCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGAATTTTTACTTTCGTCCGGGTTCCGGAT                                                                                                   >  2:628156/1‑149
                                        TCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCATCCGGaacccggacgaaa                                                                                               <  1:541860/149‑14
                                         CACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCATCCGGaacccggacgaaag                                                                                              <  1:71891/149‑15
                                                                       TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTGCCGGATGAAACTGCCAAGGGGGGACATTACAATTGAAATTG                                                                >  2:215640/1‑149
                                                                         ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGCTGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCT                                                              >  2:88908/1‑149
                                                                            AACCCTGTCCTATGGCTCCAGAGGACAAACATGATCTCACATAAGGGACCAGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTGTCTTTCGGCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGT                                                           <  1:226598/149‑1
                                                                              CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTT                                                         >  2:464041/1‑149
                                                                              CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTT                                                         >  1:171140/1‑149
                                                                                  GTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGT                                                     <  1:179258/149‑1
                                                                                               AGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCGACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTA                                        >  2:292482/1‑149
                                                                                                 ATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAGATGGAATTGCTTGTTTGTGTGTGCACAGCATTAAC                                      <  2:432745/149‑1
                                                                                                 ATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAAC                                      <  1:560883/149‑1
                                                                                                    ACAAACATGATCTCATATCAGGGACATGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAG                                   <  1:421619/149‑1
                                                                                                      AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACCTTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCT                                 >  2:205459/1‑149
                                                                                                      AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCT                                 >  2:580739/1‑149
                                                                                                             ATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATG                          <  1:384786/149‑1
                                                                                                                TCATATCAGGGACTTGTTCGCCCCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGC                       <  1:356617/149‑1
                                                                                                                         GGACTTGCTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACCTTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCCGTATGAGCCGCCAGTAA              >  2:530067/1‑149
                                                                                                                          gatTTGTTCGCACCTTCCTTAGGGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAG             <  2:359851/146‑1
                                                                                                                           actttgTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATGGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAGT            <  2:312713/143‑1
                                                                                                                             TTGTTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAGTGA          >  1:550818/1‑149
                                                                                                                                TTCGCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAGTGATAA       <  1:38642/149‑1
                                                                                                                                TTCGCACCTTCCTTAGGGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAGTGATAA       <  2:197704/149‑1
                                                                                                                                 TCGCACCTTCCTTAGTGCAACTCATATGGATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAGTGATAAT      <  2:683065/149‑1
                                                                                                                                   GCACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAGTGATAATAT    >  2:439393/1‑149
                                                                                                                                     ACCTTCCTTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCCCATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTACCCAGCTCAGTATGAGCGGCCAGTAAGGGATACTATAT  >  1:119998/1‑149
                                                                                                                                                                                                                                                                                          
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2283453‑2283592
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGTGCAACTCATATGCATTTTTACTTTCGTCCGGGTTCCGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAGCCGCCAGTAAGTGATAATATAT  <  CP009273/2400260‑2400119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.