Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 468,144 Δ1 bp coding (91/339 nt) glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273468,1440A.97.8% 399.7 / 0.7 92coding (91/339 nt)glnKnitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base . (51/38);  total (53/39)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCA  >  CP009273/468001‑468285
                                                                                                                                               |                                                                                                                                             
aaTACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAAACAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATGCAGGGCCTGAACGTCCCGAAg                                                                                                                                         >  1:1620836/1‑149 (MQ=255)
 gtACCTTCTTCGTGTGGCAGAACCATTACCGGATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGt                                                                                                                                        <  2:1821732/148‑1 (MQ=255)
     cttcttCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGaaa                                                                                                                                    >  2:1650193/1‑149 (MQ=255)
     cttcttCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGaaa                                                                                                                                    >  1:500165/1‑149 (MQ=255)
     cttcttCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGaaa                                                                                                                                    <  1:1558037/149‑1 (MQ=255)
     cttcttCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCACGACCTAACCGTCCCGAAGTGcaa                                                                                                                                    >  1:1875422/1‑149 (MQ=255)
      ttcttcGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGGTAATCAAACCATTCAAGCTGGAAGAAGTTCGTGAAGCGTTATCTTGCATTGGTATTCAGGGCCTGAACGTCCCGAAGTGAAAg                                                                                                                                   >  2:1784503/1‑149 (MQ=255)
         ttcGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGtt                                                                                                                                >  2:1816036/1‑149 (MQ=255)
                 gCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGt                                                                                                                        <  2:1855255/149‑1 (MQ=255)
                  cAGAACCATTACCGAATTCTGACCGCAGGGGATGTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGTAGCGTTACCTTCCATTGGTATTCAGGGCCTGCCCGTCCCGAAGTGAAAGGTTTCGGGCGTc                                                                                                                       <  1:1202445/149‑1 (MQ=255)
                   aGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCa                                                                                                                      >  2:1892408/1‑149 (MQ=255)
                     aaCCCTTACCGAAGTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTCATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGa                                                                                                                    <  2:1290130/149‑1 (MQ=255)
                     aaCCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGGGACGGTGATAATCAAGCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCGTCCATTGGTATTCAGGGACTGACCGTGACGAAGTGAAAGGTTTCGGGCGTCAGa                                                                                                                    >  1:156229/1‑149 (MQ=255)
                       ccATTACCGGATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATTTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGaaa                                                                                                                  <  2:647380/149‑1 (MQ=255)
                        cATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGCTAATCAAACCATTCAAGCTGGTAGACGTTCGTGAAGCGTTATCTTCCATTGTTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAg                                                                                                                 >  1:100835/1‑149 (MQ=255)
                         aTTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGTCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAGAgg                                                                                                                >  1:1059744/1‑149 (MQ=255)
                          ttACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGACGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAggg                                                                                                               <  1:1370704/149‑1 (MQ=255)
                              cGAATTCTGACCGGAGGGGCTCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGCAGTGAAAGGTTTCGGGCGTCAGAAAGGGCGTg                                                                                                           >  2:1232629/1‑149 (MQ=255)
                              cGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCTGGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATg                                                                                                           >  2:416524/1‑149 (MQ=255)
                                 aTTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCg                                                                                                        >  1:1223620/1‑149 (MQ=255)
                                   gcTGACCGGGGGGGATCTATGAAGCTGGTGACCGTGATAAACAAACGAGTCGAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCTGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAg                                                                                                      <  2:1725578/148‑1 (MQ=255)
                                     tGACCGGAGGGGGTCTATGAAGCCGTTGACCGTGATCATCAAACCATTCAAGCCGGAAGACGTTCGTGAATCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCt                                                                                                    <  2:1344615/149‑1 (MQ=255)
                                      ggccGGAGGGGTTCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTg                                                                                                   <  2:443010/147‑1 (MQ=255)
                                            aGGGGATTTATGCGGCTGGTGGCCGTGATAATCAACCCATTCAAGCTGGAAGACGTTCGTGGAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCgg                                                                                             <  2:587649/149‑1 (MQ=255)
                                               ggATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAATCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACAggggg                                                                                          >  2:914833/1‑149 (MQ=255)
                                               ggATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGCTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCggggg                                                                                          >  2:914756/1‑149 (MQ=255)
                                               ggATCTATGAAGCTGGTGACCGTGATAATCAAACCATCCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGGGCTGAACCggggg                                                                                          >  2:914750/1‑149 (MQ=255)
                                                gctCTATGGAGCTGGTGACGCGGATAATCCTACCATTCAAGGCGGAGGTCGTTCGTGAAGCGTTATCTTCCATTGATATTCAGGGCCTTACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTTTACCgggggg                                                                                         <  1:319702/147‑1 (MQ=255)
                                                gATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCgggggg                                                                                         <  1:766949/149‑1 (MQ=255)
                                                  tCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGCCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCg                                                                                       <  1:1262565/149‑1 (MQ=255)
                                                  tCTATGAAGCTGGTGACCGGGATAATCAAACCATTCAACCTGTAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGTCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCgggggcgg                                                                                       <  1:856238/149‑4 (MQ=255)
                                                  tCTATGAAGCGGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCg                                                                                       <  2:1283422/149‑1 (MQ=255)
                                                    tctGAAGCTGGTGACCGTGATAATCAAACTATTCAAGCTGGAAGACGTTCGTGCAGCGTTATCTTCCATTGGTAGTCACGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGa                                                                                     <  1:413786/147‑1 (MQ=255)
                                                        aaGCTGGGGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATAc                                                                                 <  2:291922/149‑1 (MQ=255)
                                                           cTGGTGATCGTGATAATCAAACCATTCCAGCTGGAAGACGTACGTGAAGCGTTCTACCGCGTTGGTATTCAGGGCCTGACCGTCCCCAAGTGAAAGGTTCCGGTCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGc                                                                              <  1:1918225/149‑1 (MQ=255)
                                                           cTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGc                                                                              >  2:1496639/1‑149 (MQ=255)
                                                           cTGGGGACCGTGATAATCAAACCACTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGc                                                                              <  1:1092401/149‑1 (MQ=255)
                                                                  ccGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAAtt                                                                       <  1:980616/149‑1 (MQ=255)
                                                                   cGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACGGCGGCAAttt                                                                      >  1:1643331/1‑149 (MQ=255)
                                                                   cGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACCGCGTCAAttt                                                                      >  2:1593131/1‑149 (MQ=255)
                                                                     tGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGAGGCGGAATACAGCGTCAATTTcc                                                                    >  1:1231401/1‑149 (MQ=255)
                                                                           tCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCaa                                                              >  1:63437/1‑149 (MQ=255)
                                                                             aaaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGGATACAGCGTCAATTTCCTGCCaaac                                                            >  2:1215809/1‑148 (MQ=255)
                                                                             aaaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCTaaaa                                                            >  2:1431260/1‑149 (MQ=255)
                                                                             aaaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCAAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCaaaa                                                            >  1:1383733/1‑149 (MQ=255)
                                                                              aaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAg                                                           <  1:1034234/149‑1 (MQ=255)
                                                                              aaCCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAg                                                           >  2:880207/1‑149 (MQ=255)
                                                                                  aTTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAGTACAGCGTCCAGTTCCTGCCGAAAGTaaa                                                       >  2:218681/1‑149 (MQ=255)
                                                                                     cAAGCTGGAAGACGTTCGTGAAGAGTTATCTTCCATTAGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAAt                                                    >  1:1287393/1‑149 (MQ=255)
                                                                                       aGCTGGAAGACGTTCGTGAAGCGTTATCTTCAATTGCATGATAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTg                                                  >  1:474497/1‑149 (MQ=255)
                                                                                         cTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTCCCAAAAGTAAAAATTGAt                                                >  2:563497/1‑149 (MQ=255)
                                                                                         cTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATCCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGAt                                                <  2:1757337/149‑1 (MQ=255)
                                                                                             aaGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTgg                                            <  2:1602659/149‑1 (MQ=255)
                                                                                             aaGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGCCAGAAAGGGCATGCCGAGCGGTACCGGGGGGCGGAAAACAGCGTCAATTACCTGCCAAACGTAAACATTAAGGTgg                                            >  1:1405935/1‑149 (MQ=255)
                                                                                                aCGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGa                                         >  1:996514/1‑149 (MQ=255)
                                                                                                aCGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCGGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAAGACAGCGTCAATTTCGTGCCAAAAGTAAAAATTGATGTGGGGa                                         >  2:1792803/1‑149 (MQ=255)
                                                                                                 cGTTCGTGAAGCGTTTTCTTCCATTGGTATTCAGTGCCTGACCGTCCCGAATTTAAAGTTTTCGGGCGTCAGAAATGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGAt                                        <  1:1784503/149‑1 (MQ=255)
                                                                                                  gTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGCGCTGTACCGGGGGGCGGAATGCAGCGTCAATTTCCTGCCACAAGTAAAAAATGCGGTGGCGAtt                                       >  1:87506/1‑149 (MQ=255)
                                                                                                  gTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGAtt                                       >  1:1005250/1‑149 (MQ=255)
                                                                                                   ttCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGACATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTg                                      >  1:148156/1‑149 (MQ=255)
                                                                                                    tCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGc                                     >  2:632043/1‑149 (MQ=255)
                                                                                                    tCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGAGGTGGCGATTGc                                     >  1:489900/1‑149 (MQ=255)
                                                                                                      gTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtg                                   >  1:1819184/1‑149 (MQ=255)
                                                                                                       tGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtga                                  >  1:1943299/1‑149 (MQ=255)
                                                                                                        gAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtgat                                 <  2:1223620/149‑1 (MQ=255)
                                                                                                        gAAGCCTTATCTGCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCTGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCtgat                                 <  2:1422167/149‑1 (MQ=255)
                                                                                                               tATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAAc                          <  2:500165/149‑1 (MQ=255)
                                                                                                               tATCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGGAAAAATTGAGGTGGCGATTGCTGATGACCAAc                          >  2:1198026/1‑149 (MQ=255)
                                                                                                                aTCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTTATGACCAAcg                         >  1:61255/1‑148 (MQ=255)
                                                                                                                 tCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTc                        >  1:91618/1‑149 (MQ=255)
                                                                                                                 tCTTCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTc                        <  1:1181759/149‑1 (MQ=255)
                                                                                                                   ttCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGa                      >  1:1772412/1‑149 (MQ=255)
                                                                                                                   tgccATTGGTATTCCNGGCCTGACCGTCCTGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGAGGAATACAGCGTCGATGGCCTGTCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGa                      <  1:1844188/147‑1 (MQ=255)
                                                                                                                    tCCATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACCGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGAt                     <  1:560036/149‑1 (MQ=255)
                                                                                                                    tCCATTGGTATTCAGGGCCTGACCTGCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGAt                     <  2:922688/149‑1 (MQ=255)
                                                                                                                      cATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGa                   >  1:241480/1‑149 (MQ=255)
                                                                                                                      cATTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGa                   >  2:1829397/1‑149 (MQ=255)
                                                                                                                       aatGGTATTCAGGGACTGACCGTCCCGAAGTGAAAGGGTGCGGGCGTCAGAAAGGGCATGCCGAGCTGTGCCGGGGGGCGGAAGACAGGGTCAATTTCCGGCCGAACGTAAAGATTGATGTGGCGGGTCCTGATGACCAAGTCGGTGaa                  >  2:325236/3‑149 (MQ=255)
                                                                                                                       aTTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGaa                  >  2:1359886/1‑149 (MQ=255)
                                                                                                                       aTTGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGaa                  >  1:1057567/1‑149 (MQ=255)
                                                                                                                         tGGTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGt                >  2:479969/1‑149 (MQ=255)
                                                                                                                           gTATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGa              <  1:1892408/149‑1 (MQ=255)
                                                                                                                            tATTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGAt             <  1:602160/149‑1 (MQ=255)
                                                                                                                             aTTCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATc            <  2:1270509/149‑1 (MQ=255)
                                                                                                                              ttCAGGGCCTGACCGTCCCGAAGTGAACGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCg           >  1:1857230/1‑149 (MQ=255)
                                                                                                                              ttCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGAtgg           >  2:588826/1‑147 (MQ=255)
                                                                                                                              ttCAGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCg           <  1:363179/149‑1 (MQ=255)
                                                                                                                                 aGGGCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGatc        >  2:369921/1‑148 (MQ=255)
                                                                                                                                   ggCCTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATc      <  1:1797716/149‑1 (MQ=255)
                                                                                                                                     ccTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGt    <  1:1111956/149‑1 (MQ=255)
                                                                                                                                      cTGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCGGAGCTGTAGCGGGGGGCGGAATAGAGCGTAAATTTCCTGCCAAAAGTAAAAATTGATGTGGGGATTGCTGATGACCAACCCGATGAAGTGACCGATATAGTc   >  1:433003/1‑149 (MQ=255)
                                                                                                                                       tGACCGTCCCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACTGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCa  <  2:1287393/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                             
AATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCA  >  CP009273/468001‑468285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: