Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 982,560 T→A intergenic (‑122/+481) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273982,5600TA97.9% 139.3 / ‑5.7 47intergenic (‑122/+481)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (22/24);  minor base G (1/0);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACA  >  CP009273/982417‑982705
                                                                                                                                               |                                                                                                                                                 
ccAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAtt                                                                                                                                              >  2:820776/1‑149 (MQ=255)
   gAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTAcc                                                                                                                                                 <  1:865507/143‑1 (MQ=255)
              ttCATCATTATTTATTACCCTCATGGTTTTTTTTTTTTTTTTTACCACTGCCGTCAATAATTTCTGTCAATAAAAATTTACGGAACTATTGATGAGGGTTTGGTGTCTTTTTGTGTCTGCAGTCTTCTTACCATTCATACTaa                                                                                                                                      >  1:984321/1‑143 (MQ=255)
              ttCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTc                                                                                                                                >  2:1017241/1‑149 (MQ=255)
                  tcatTATTTATTACCCTCATGGTTTTTTTTTTTTTTCCTGCCACTGACGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGATTTTCGTGTCTTTATGTGTCTGTAGGCATCTTACATTTCAAATTGACGtt                                                                                                                                  >  1:486459/1‑143 (MQ=255)
                     ttatttatTACCCTCATGGTTTTTTTTAATACAGCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCg                                                                                                                               >  1:496944/1‑143 (MQ=255)
                               cccTCATGGTTTTTTTTATGACATCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTGCCCTTCACACTAGCGTTTCGCGACCGTGaa                                                                                                                     >  1:743008/1‑143 (MQ=255)
                                ccTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGaaa                                                                                                                    <  1:598674/143‑1 (MQ=255)
                                   cATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaa                                                                                                           >  2:840746/1‑149 (MQ=255)
                                        ttttttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTGGCTACGGTGAACGTGCTACaaggata                                                                                                      >  2:156099/1‑149 (MQ=255)
                                           tttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaag                                                                                                         >  1:950677/1‑143 (MQ=255)
                                            ttttttttttttttccACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATTTGTCTGCAGTCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAATTGTTAAaaaga                                                                                                        >  1:946871/15‑143 (MQ=255)
                                                  gACACCTGCCCCTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCAGCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGt                                                                                            <  2:304351/149‑1 (MQ=255)
                                                        tGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaa                                                                                      <  2:554165/149‑1 (MQ=255)
                                                        tGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAAACACCTTACCGTTCAAGCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaa                                                                                      >  2:1253/1‑149 (MQ=255)
                                                        gtccACTGGCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGt                                                                                            <  1:1061572/141‑1 (MQ=255)
                                                           ctcTGTCGTCAATAGGTTCTGTCGATAAAACTTTACGGAGCTATTGATGTGCGTTTGGTGTCTTTATGTGTCTGGCGGCGTCTTACCGTTCAGACTAGCGTTTCGCTTCCGTGAGAGTGCTACTACGATAAAGATTTGGTTTc                                                                                         <  1:970321/141‑1 (MQ=255)
                                                           cgcTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTAAGTGTCTGCAGGCATCTTACCATTCAAACTTACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTc                                                                                         <  1:80521/141‑1 (MQ=255)
                                                           cACTGCCGTCAATAAGTTCTGTCAATAAAAAGTTACGTGACTATTGATGAGAGTTTGGTGTCTGTAAGTGTCTGCAGGTATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAATGATAAAGATTTGGTTTc                                                                                         <  1:80370/143‑1 (MQ=255)
                                                                ccGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaaa                                                                                    <  1:1087922/143‑1 (MQ=255)
                                                                ccGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaa                                                                              >  2:15879/1‑149 (MQ=255)
                                                                 cGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACAAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaaa                                                                             <  2:734089/149‑1 (MQ=255)
                                                                  gTCAATACGTTCTGTCAATAAAAATTTACGGTGCTATTGATGAGAGTATGTTGCGGTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAATATAAAGATTTGGTTTCAAAAAGAAAAAat                                                                            <  2:745984/149‑1 (MQ=255)
                                                                  gTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAGAAAGa                                                                                  >  1:871656/1‑143 (MQ=255)
                                                                   tCAATGAGTTCTGTCAATAAAAATTTACGGAACTATTGATGGGAGTTGGGTGGCTTTATGTGTCTGCAGGGATCTTACCATTCAAACTAACGTTTCGCTACGGTGAAATTGCTACAAAGAAAAAGATTTGGTTTGAAAAAGaa                                                                                 >  1:294498/1‑143 (MQ=255)
                                                                        aaGTTCTTTCAATAAAAATTTACCGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACAAACGTTTCACAACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTaa                                                                      <  2:816684/149‑1 (MQ=255)
                                                                               cacAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAg                                                               <  2:199334/147‑1 (MQ=255)
                                                                                      aaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAAtt                                                        >  2:1040393/1‑149 (MQ=255)
                                                                                         aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCGGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa                                                     <  2:518911/149‑1 (MQ=255)
                                                                                         aTTTACGGAACCATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCGGGCCTCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa                                                     <  2:567486/149‑1 (MQ=255)
                                                                                         aTTTACGAAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa                                                     <  2:891207/149‑1 (MQ=255)
                                                                                             aCGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACAAAAAGTAAAAttt                                                       >  1:563933/1‑143 (MQ=255)
                                                                                               ggAACTATTGGTGAGAGGTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGAAAACAAAAGTAAAATTTaa                                                     >  1:908942/1‑143 (MQ=255)
                                                                                                   cTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTACCCAAAAGTAAAATTTAAGGAACTTtgt                                           <  2:701328/149‑1 (MQ=255)
                                                                                                     aTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGa                                         <  2:138065/149‑1 (MQ=255)
                                                                                                             gagctcggTGTCTTTATGTGTCTGCAGGCATTTTACCATTCATACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAac                                       <  1:29988/137‑1 (MQ=255)
                                                                                                                tttGGTGTCTTTAGGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata                              >  2:808791/1‑149 (MQ=255)
                                                                                                                      gtCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCa                        <  2:1136473/149‑1 (MQ=255)
                                                                                                                       tCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACAGTCatat                             >  1:242531/1‑143 (MQ=255)
                                                                                                                        cTTTATGTGTCTGCAGGCATTTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATa                      <  2:7979/149‑1 (MQ=255)
                                                                                                                              gtgtCTGCAGGCATCTTACCATCCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTAAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATa                      <  1:746782/143‑1 (MQ=255)
                                                                                                                                gtCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATaga                    >  1:544856/1‑143 (MQ=255)
                                                                                                                                   tGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGCAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatg           >  2:819937/1‑149 (MQ=255)
                                                                                                                                    gCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGggatga          >  2:900808/1‑149 (MQ=255)
                                                                                                                                     cAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAAGTTAAGGAACTTTGTGAACACCGTAATATTTCCAGAGAGTCGAgaagat         >  2:138030/1‑149 (MQ=255)
                                                                                                                                         cATCTTACCATTCAAACTAACGGTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAttt     <  2:237182/149‑1 (MQ=255)
                                                                                                                                            cTTACCATTCAAACTAACGTTCCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACa  <  2:330317/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACA  >  CP009273/982417‑982705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑