Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,527,564	G→C	R47P (CGT→CCT)	ptsI →	PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,527,564	0	G	C	100.0%	241.1 / NA	73	R47P (CGT→CCT)	ptsI	PEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (35/38);  total (35/38)

TAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAATCATAGCC  >  CP009273/2527418‑2527697                                                                                                                                                   |                                                                                                                                      tAGGGTTATGATCTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTg                                                                                                                                     <  2:70864/149‑1 (MQ=255)   gggTTATGATTTCAGCCATTTTAGCATCCCCGCGCATCGCCTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGcc                                                                                                                                   <  2:912266/149‑1 (MQ=255)       tacgATTTCAGGCATTTTAGCCTCCCCGCGTAACGGTCAATGCATATCGCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAATTTGAACGTTTTCTGAGCGGTCCTGCCAAgg                                                                                                                               <  2:621808/146‑1 (MQ=255)          gATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCAt                                                                                                                            >  2:414676/1‑149 (MQ=255)          gATTTCAGGCATTTTAGCACCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTGTGAAAGAAGACGAAATTGTCATTGACCAGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCAt                                                                                                                            <  2:876436/149‑1 (MQ=255)                  gCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGACATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAg                                                                                                                    >  2:231873/1‑149 (MQ=255)                  gCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAg                                                                                                                    >  2:427715/1‑149 (MQ=255)                    aTTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGCAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGGAAGGTCCTTCCAAGGAAtgtgc                                                                                                                        >  1:532786/1‑139 (MQ=255)                          gCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGGGCGGTCCTGCCAAGGCATCAGCCCGGcgagaagc                                                                                                            >  2:58657/1‑142 (MQ=255)                          gCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCt                                                                                                                  >  1:1026835/1‑143 (MQ=255)                          gCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCt                                                                                                                  <  1:833658/143‑1 (MQ=255)                           cATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTg                                                                                                                 >  1:877530/1‑143 (MQ=255)                            aTCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGa                                                                                                          <  2:557103/149‑1 (MQ=255)                             tCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGa                                                                                                               <  1:561959/143‑1 (MQ=255)                                ccGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCaa                                                                                                      >  2:752970/1‑149 (MQ=255)                                   ggTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAAc                                                                                                   <  2:203256/149‑1 (MQ=255)                                     tATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATcc                                                                                                       <  1:453650/143‑2 (MQ=255)                                           tttCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGAACCAAACGAAAGCTg                                                                                           >  2:472437/1‑149 (MQ=255)                                           tttCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCAACCAGGTTGATAAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGCAACGATCAAAACGa                                                                                                 >  1:273529/1‑143 (MQ=255)                                               ggTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTgg                                                                                       >  2:699678/1‑148 (MQ=255)                                                gTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAACACGAAAGCTGGTGaa                                                                                      >  2:5684/1‑149 (MQ=255)                                                   aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGACAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAATGCATCAGCCCAGCTGGAAACGATCACAACGAAAGCTGGt                                                                                         <  1:655380/143‑1 (MQ=255)                                                     gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGa                                                                                       >  1:101353/1‑143 (MQ=255)                                                       tctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCg                                                                               <  2:740569/149‑1 (MQ=255)                                                        tgtcTTCTGAAAGCAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCCACTCTTGTTGAAACGATCAAAACGAAAGCTGGTGAAACGTTCgg                                                                              <  2:871145/146‑1 (MQ=255)                                                        ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAAc                                                                                    >  1:450818/1‑143 (MQ=255)                                                          gcctCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTCAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                            <  2:594228/146‑1 (MQ=255)                                                           cTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTga                                                                           <  2:669536/149‑1 (MQ=255)                                                            ttCTGAAAGAAGACGAAATTGTAATTGACCGGAAAAAAATTTTTGCCGACGGGGGTGACCGGGAAGTTGAACGCTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTc                                                                                >  1:747477/1‑143 (MQ=255)                                                             tCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAATTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaag                                                                         <  2:12310/149‑1 (MQ=255)                                                                 aaaGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaa                                                                     >  2:806907/1‑149 (MQ=255)                                                                  aagaagACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaa                                                                          <  1:239657/143‑1 (MQ=255)                                                                  aagaagACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGATTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaa                                                                          >  1:201757/1‑143 (MQ=255)                                                                     aagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACAAGGTTGATCAGGCAGTAGAACGTTCTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                                                       >  1:762984/1‑143 (MQ=255)                                                                       gaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGCAAGCTGGTGAAACGTTCGGTGAAGaaaa                                                                     >  1:582898/1‑143 (MQ=255)                                                                        aCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGcc                                                              <  2:304508/149‑1 (MQ=255)                                                                        aCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTACTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGGTCGGTGAGGAAAAAGAAAcc                                                              >  2:10589/1‑149 (MQ=255)                                                                            aaTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAGGAAAaagaag                                                                >  1:1030640/1‑143 (MQ=255)                                                                             aTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGc                                                               <  1:81180/143‑1 (MQ=255)                                                                             aTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCtt                                                         <  2:1017999/149‑1 (MQ=255)                                                                               tGTCATTGACCGGAAAAGAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAACCGAAAGCTGGGGGAACACTCGGTGAAGAAAAAGAAGCCa                                                             >  1:488131/1‑143 (MQ=255)                                                                                gTCCTTGACCGGAAAAAAATTTCTGCCGACCAGGTTGCTCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGa                                                      <  2:433358/149‑1 (MQ=255)                                                                                    ttGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGCAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAggg                                                  >  2:263563/1‑149 (MQ=255)                                                                                       gccGGAAAAAAATTTCTGCCGACCAGGGTGATCAGGTAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAATCGTTCGGTGAAGAAAAAGAAGCCATCTTTGaa                                                     <  1:115161/142‑1 (MQ=255)                                                                                       aCCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGaa                                                     <  1:751753/143‑1 (MQ=255)                                                                                        ccGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAAAAGCCATCTTTGacg                                                    >  1:968380/1‑141 (MQ=255)                                                                                           gAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGATCGTTTTCCGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta                                           <  2:622924/149‑1 (MQ=255)                                                                                              aaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCata                                              >  1:499294/1‑143 (MQ=255)                                                                                                   cctctcCCGACCAGTCTGATCAGGAAGCTGAACGTTTTCCGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtg                                         <  1:178806/141‑1 (MQ=255)                                                                                                      cTGCCGACCAGGGTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgcccttcaaa                                <  2:1109764/149‑9 (MQ=255)                                                                                                        gCCGACGAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATgctgct                                    <  1:631863/143‑1 (MQ=255)                                                                                                         ccGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGAt                             <  2:1114143/149‑1 (MQ=255)                                                                                                           gACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATga                           >  2:1121843/1‑149 (MQ=255)                                                                                                              cAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGa                              >  1:790950/1‑143 (MQ=255)                                                                                                              cAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGa                              >  1:1085679/1‑143 (MQ=255)                                                                                                               aGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGACGAt                             >  1:151967/1‑143 (MQ=255)                                                                                                                  ttGATCAGTCGGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGCAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATgag                          <  1:760076/143‑1 (MQ=255)                                                                                                                  ttGATCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCATCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTg                    <  2:875187/149‑1 (MQ=255)                                                                                                                     aTCAGGAAGTTGAACGTTTTCTGGGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATgaggag                       <  1:399272/143‑1 (MQ=255)                                                                                                                     aTCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAg                 >  2:879706/1‑149 (MQ=255)                                                                                                                      tCAGGAAGTTGAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGc                      >  1:382470/1‑143 (MQ=255)                                                                                                                       cAGGAAGTTGAACGTTTTCTGAGCGGTCCGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGACGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCa               <  2:529720/149‑1 (MQ=255)                                                                                                                          gAAGGTGAACGTATTCTGAGCGGTCCTGCCAAGGCAACAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGa                  <  1:212387/143‑1 (MQ=255)                                                                                                                            ttgtGAATGTTTTCTGGGCGGTCCTGCCAAGGCATCAGCCCAGGTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCCTCTTCCGGCAGCATATTCTGCTGCTCGAAGACTAGGAGCTGGTGCAGGaaa          <  2:868191/146‑1 (MQ=255)                                                                                                                             gTTGAACGGTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTACCTGAAGAAAAAGAAGAAAAAAAAAAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCa               <  1:1044403/143‑1 (MQ=255)                                                                                                                               ggAACGTTGTCTGAGCGGTCCGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAATCa       <  2:936155/148‑1 (MQ=255)                                                                                                                                gAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGa            >  1:114687/1‑143 (MQ=255)                                                                                                                                gAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAATCAt      >  2:333508/1‑149 (MQ=255)                                                                                                                                gAACGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAATCAt      <  2:957408/149‑1 (MQ=255)                                                                                                                                 aaCGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGaa           <  1:916693/143‑1 (MQ=255)                                                                                                                                  aCGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGCGCAGGAAATCATAg    >  2:1063115/1‑149 (MQ=255)                                                                                                                                  aCGTTTTCTGAGCGGTCCTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGATGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGaaa          >  1:984112/1‑143 (MQ=255)                                                                                                                                          tGAGCGGTCCTGCCAAGGCAGCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAATCATAGcc  <  1:87886/143‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                      TAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAATCATAGCC  >  CP009273/2527418‑2527697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑