Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,370,282 C→T W166* (TGG→TGA sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,370,2820CT98.6% 226.2 / NA 71W166* (TGG→TGAsspAstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (29/40);  minor base G (1/0);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCG  >  CP009273/3370136‑3370423
                                                                                                                                                  |                                                                                                                                              
aCAGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTca                                                                                                                                              <  2:934596/149‑1 (MQ=255)
aCAGACTAACTCCGGCCCAGACGCATTGCACGTTCTGCTACAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTca                                                                                                                                              <  2:306790/149‑1 (MQ=255)
 cAGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTcac                                                                                                                                             >  2:107961/1‑149 (MQ=255)
        aCTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAGAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTcaca                                                                                                                                            >  1:112189/1‑143 (MQ=255)
          tCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGACGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCCCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGa                                                                                                                                   <  2:125490/149‑1 (MQ=255)
          tCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGCACTCGATTCCCCGTTGCGGCAGACGTCACAGCCGCGGa                                                                                                                                   >  2:881436/1‑149 (MQ=255)
          tCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGa                                                                                                                                   <  2:100188/149‑1 (MQ=255)
                ccAGCCGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGa                                                                                                                                   <  1:614591/143‑1 (MQ=255)
                ccAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAGCTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGa                                                                                                                                   <  1:557016/143‑1 (MQ=255)
                ccAGACGCATTTCACGTTCTGCTACAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGa                                                                                                                             >  2:776404/1‑149 (MQ=255)
                  aGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGCGTCACGCTGAGAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATa                                                                                                                           <  2:227441/149‑1 (MQ=255)
                  aGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCCGACGTCACAGCAGCGGAGc                                                                                                                                 >  1:1026631/1‑143 (MQ=255)
                   gACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAg                                                                                                                          >  2:979917/1‑149 (MQ=255)
                         tttCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCg                                                                                                                    >  2:134037/1‑149 (MQ=255)
                           tCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCACAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCa                                                                                                                        >  1:534313/1‑143 (MQ=255)
                             aCGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCa                                                                                                                >  2:319983/1‑149 (MQ=255)
                              cGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATc                                                                                                                     <  1:400977/143‑1 (MQ=255)
                               gTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCAGCGCAGGGGAGCAAGATAGCAATCGACCCgg                                                                                                              >  2:388676/1‑149 (MQ=255)
                               gTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAgg                                                                                                              <  2:137641/149‑1 (MQ=255)
                                  cTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCCGGTGGCTATACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTg                                                                                                           >  2:287455/1‑149 (MQ=255)
                                    gcctCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCAACCAGGCTGaa                                                                                                         <  2:1052739/146‑1 (MQ=255)
                                    gCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCGCAAAGACGCGGGTCAGATAGCCTTGCAGCTCTGTCGCACCCGGGCCGCTGAACTCGATGCCCAGGTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGaa                                                                                                         <  2:540933/149‑1 (MQ=255)
                                      ttCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCCGCTCTTTCGCGCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACt                                                                                                       >  2:984882/1‑149 (MQ=255)
                                        cAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATAAAAGCAAACGCCGCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCa                                                                                                     <  2:34312/149‑1 (MQ=255)
                                          gTTAAAAAAGCAAGGAAAGAGTCACGCTCAAAGACCCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATc                                                                                                   <  2:563049/149‑1 (MQ=255)
                                              aagaagCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCa                                                                                                     <  1:377140/143‑1 (MQ=255)
                                                 aagCAAGGACAGAGTCACGCGCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAgg                                                                                            <  2:741777/149‑1 (MQ=255)
                                                 aagCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGTTAGCAATCGACCAGGCTGAACTCATCg                                                                                                  <  1:279845/143‑1 (MQ=255)
                                                   gCAAGGAAAGAGTCACGCTCAAAGACGCGGGGCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCGAGATAGCAATCGACCAGGCTGAACTCGTCGcc                                                                                                >  1:459500/1‑142 (MQ=255)
                                                                  cGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGAcc                                                                           <  2:495905/149‑1 (MQ=255)
                                                                  cGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTGTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGAcc                                                                           <  2:40131/149‑1 (MQ=255)
                                                                   gCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTc                                                                                <  1:764985/143‑1 (MQ=255)
                                                                      cAAAGACGCGGGTCATATAGCCTTTCCGCTCTTTCGCACCCGGGCCGCTGACCTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGa                                                                             <  1:958914/143‑1 (MQ=255)
                                                                       aaaGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGAc                                                                            >  1:193368/1‑143 (MQ=255)
                                                                         aGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGGTGGCATTCGACCAGGCTGACCTCATCGCCCAGGAAGTACGGCTTCTAACCGAAAAcc                                                                    >  2:977487/1‑149 (MQ=255)
                                                                         aGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCg                                                                          <  1:1035468/143‑1 (MQ=255)
                                                                           aCGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCgg                                                                  >  2:711226/1‑149 (MQ=255)
                                                                            cgcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgc                                                                 <  2:289919/149‑1 (MQ=255)
                                                                             gcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcg                                                                <  2:992369/149‑1 (MQ=255)
                                                                             gcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCGTCTGACCGAAGa                                                                      >  1:839372/1‑143 (MQ=255)
                                                                               gggTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGGCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGCACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCa                                                              >  2:652089/1‑149 (MQ=255)
                                                                                ggTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCCGTGGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCaa                                                             >  2:233212/1‑149 (MQ=255)
                                                                                   cATATACCCTTTCAGCTCTTCCGCACCCCGGCCGCTGAACTCGATGCCCAGTAGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcg                                                                <  1:121911/143‑1 (MQ=255)
                                                                                    atatAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGGAGACCGGCCCAATCGc                                                         >  2:331769/1‑149 (MQ=255)
                                                                                      ctcgCCTTCCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTATGACCGAAGACCGGCGCAATCGCca                                                       <  2:595985/146‑1 (MQ=255)
                                                                                      ataGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATGGCAATCGACCAGGCTGAACCCATCGCTCAGGAAGGACGGCTTCTGACCGAAGGCCGGCGCAGACGCca                                                       >  2:36786/1‑149 (MQ=255)
                                                                                       taGCCTTTCAGCTCGTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGCCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCcag                                                      <  2:776758/149‑1 (MQ=255)
                                                                                          ccTTTCAGCTCTTTCGCACCCGGGCCGCTCAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGATCGAAGACCGGCGCAATCGCcagcag                                                   >  2:723354/1‑149 (MQ=255)
                                                                                          ccTTTCACCTCTTTCGCCCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGc                                                         <  1:540557/143‑1 (MQ=255)
                                                                                            tttCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGtt                                                 <  2:933151/149‑1 (MQ=255)
                                                                                            tttCAGCTCTTTCGCACCCGGGCCGCTGAACCCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCca                                                       <  1:344110/143‑1 (MQ=255)
                                                                                            tttCAGCTCTTTCCCACCCGGGCCGCTGAACTCGATGCCAAGTTGCGGAAGACGCTACAGCAATGGATCAAGGTAGCAATCGACCAGGCTGAACTCATCGACCAGGAAGTACGGCTCCTGACCGAAGCCCGGCGCCATCGCca                                                       >  1:656138/1‑143 (MQ=255)
                                                                                             ttCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttc                                                <  2:355814/149‑1 (MQ=255)
                                                                                                         gCACCCGGGCCGCTGACCTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTg                                    <  2:783207/149‑1 (MQ=255)
                                                                                                          cACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGGTCTTCgcgc                                         >  1:435381/1‑143 (MQ=255)
                                                                                                           ccccGGGCCGCTGAACGCGACGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCa                                        <  1:498395/142‑1 (MQ=255)
                                                                                                                ggCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGc                                   <  1:351880/143‑1 (MQ=255)
                                                                                                                     cTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCGGCAGTTCTTCGCGCAGTTGCTTCCg                              >  1:596199/1‑143 (MQ=255)
                                                                                                                     cTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTGGCGCGGTTGGTTACg                              >  1:430287/1‑143 (MQ=255)
                                                                                                                     cTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACg                              >  1:865950/1‑143 (MQ=255)
                                                                                                                      ggAACGCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCa                       <  2:1012618/148‑1 (MQ=255)
                                                                                                                       gctctctctGCTCAGTTGCTGCAGACGTCACAGCAGCGGAGCAAGACAGCAATCGACCAGGCGGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCAt                      <  2:269232/141‑1 (MQ=255)
                                                                                                                         aCTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCg                          <  1:922229/143‑1 (MQ=255)
                                                                                                                         aCTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTGCTTCGCGCAGTTGCTtacgtacg                          >  1:393670/1‑143 (MQ=255)
                                                                                                                          cTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCgg                         <  1:836609/143‑1 (MQ=255)
                                                                                                                           tCGATGCCCAGTTGAGGCAGACGTCACGAAGTGCCAACTATATAGCAATAGACCAGGCTGAACTCATCGCTCAGGGAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGc                  >  2:872954/1‑149 (MQ=255)
                                                                                                                                 cccAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGa            <  2:530732/149‑1 (MQ=255)
                                                                                                                                   cAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGTAGTTGCTTACGTGCGGCATCTGCTTCAGAAg          >  2:47855/1‑149 (MQ=255)
                                                                                                                                      ttGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAg             >  1:653547/1‑143 (MQ=255)
                                                                                                                                           gCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCg  <  2:123598/149‑1 (MQ=255)
                                                                                                                                            cAGGCGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGACCTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTg       <  1:58607/143‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
ACAGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCG  >  CP009273/3370136‑3370423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: