Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,683,547 G→T V239L (GTG→TTG)  manA → mannose‑6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,683,5470GT97.5% 250.4 / NA 80V239L (GTG→TTG) manAmannose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (36/41);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAAC  >  CP009273/1683402‑1683691
                                                                                                                                                 |                                                                                                                                                  
gCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGACGACAGCGGGCTGTTCTCCCCGCTATTGCTGAATGTGGTGa                                                                                                                                                 >  1:1296715/1‑149 (MQ=255)
 cAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGaa                                                                                                                                                <  1:1892372/149‑1 (MQ=255)
   gggTGAAGAAAAATCCCGCGCGCTGGCGCTTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAAt                                                                                                                                              >  1:1425820/1‑149 (MQ=255)
      tGAAGCAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGGTCTCCCCGCTATGGCTGAAGGTGGTGAACTTGa                                                                                                                                           >  1:115353/1‑149 (MQ=255)
      tGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGa                                                                                                                                                 <  2:168460/143‑1 (MQ=255)
      tGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGGGTTGAAATTGa                                                                                                                                           >  1:1625969/1‑149 (MQ=255)
         agaaAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTCTTGCTGAATGTGTTGAAATTGAAcc                                                                                                                                        >  1:1507067/1‑149 (MQ=255)
         agaaAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAAt                                                                                                                                              >  2:1385000/1‑143 (MQ=255)
          gaaAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCTCTATTGCTGAATGTGTTGAAGtt                                                                                                                                             >  2:1107545/1‑143 (MQ=255)
          gaaAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATGTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTGGAAAtt                                                                                                                                             >  2:739855/1‑143 (MQ=255)
           naacactccGCGCGCTGGCGCTTTTAAAATNGGCCTTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGAAAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAAGTg                                                                                                                                            <  2:1627761/136‑1 (MQ=255)
            taaaTCCCGCGGGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGa                                                                                                                                           <  2:1224134/142‑1 (MQ=255)
            aaaaTCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGa                                                                                                                                           <  2:1556331/143‑1 (MQ=255)
             aattCCCGCGTGCTGTCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAATCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGaa                                                                                                                                          <  2:299393/140‑1 (MQ=255)
              aaTCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAAc                                                                                                                                         <  2:621080/143‑1 (MQ=255)
                  ccGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTg                                                                                                                                     <  2:1182490/143‑1 (MQ=255)
                        gcTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGt                                                                                                                         <  1:39031/149‑1 (MQ=255)
                          tGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTc                                                                                                                      <  1:764514/149‑1 (MQ=255)
                               aTTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTGTTGCTGGATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGtt                                                                                                                >  1:339537/1‑149 (MQ=255)
                                ttttAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTc                                                                                                                      <  2:598702/143‑1 (MQ=255)
                                ttttAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTc                                                                                                               >  1:853689/1‑149 (MQ=255)
                                    aaaaTCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGGGATGTTCCTGt                                                                                                                  >  2:555624/1‑143 (MQ=255)
                                      aaTCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGaa                                                                                                         >  1:338368/1‑149 (MQ=255)
                                       aTCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTGTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACACTGGCGAAGCGATCTTGCTGTTCg                                                                                                              >  2:1700015/1‑143 (MQ=255)
                                          ggCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAacac                                                                                                     <  1:229158/149‑1 (MQ=255)
                                            cccTCGATAGGCAGCAGGGTGAACCGTGGCAAACGAGTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGGCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGaa                                                                                                         <  2:668896/143‑1 (MQ=255)
                                             ccTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGc                                                                                                  >  1:739880/1‑149 (MQ=255)
                                                 gATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTCAACGTTTTGAATTTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGc                                                                                              <  1:1489126/149‑1 (MQ=255)
                                                      ccAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTAcc                                                                                         >  1:577182/1‑149 (MQ=255)
                                                            gggTTAACCGTGGCAAACGACTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAAAGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAg                                                                                   <  1:670659/149‑1 (MQ=255)
                                                             ggTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAgg                                                                                  >  1:707433/1‑149 (MQ=255)
                                                             ggTGAACCGTGGCAAACGATTCGTTTAATTTCTAAATTTTACGCGAAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCt                                                                                        <  2:1046584/143‑1 (MQ=255)
                                                                  aCCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTgg                                                                             <  1:213706/149‑1 (MQ=255)
                                                                    cGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGgcg                                                                           <  1:487508/149‑1 (MQ=255)
                                                                    cGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGGTTTGAAATTTAACCTTGGCGACGGTGGTGTCCGGTTTGCTGCAAAGCCGCGAGCTGACCCTCAAGGCGTGgcg                                                                           >  1:911753/1‑149 (MQ=255)
                                                                    cGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGGCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACCCCGCACGCTTACCTGCAAGGCGTGgcg                                                                           >  1:1693235/1‑149 (MQ=255)
                                                                      tGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCt                                                                         <  1:492335/149‑1 (MQ=255)
                                                                        gCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTgg                                                                             >  2:890770/1‑143 (MQ=255)
                                                                        gCAAACGATTCGTTTAATTTCTGAACTTTACCCGGAAGACCGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTgg                                                                             <  2:1666165/143‑1 (MQ=255)
                                                                         cAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGCGgc                                                                            >  2:1541273/1‑143 (MQ=255)
                                                                          aaaCGATTCGTTCAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGaa                                                                     <  1:1506857/149‑1 (MQ=255)
                                                                          aaaCGATGCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCTCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGaa                                                                     <  1:1211526/149‑1 (MQ=255)
                                                                           aaCGATTCTCTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAATTTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCATGCTTACCTGCAAGGCGTGGCGCTGGAAg                                                                    <  1:1426373/149‑1 (MQ=255)
                                                                            aCGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTTTTCGCTGTAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGt                                                                   >  1:1095571/1‑149 (MQ=255)
                                                                              gATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGa                                                                 <  1:1680735/149‑1 (MQ=255)
                                                                                ttCGTTTAATTTTTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATg                                                               <  1:1495951/149‑1 (MQ=255)
                                                                                ttCGTTTAATTTCTTAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGaa                                                                     <  2:1132937/143‑1 (MQ=255)
                                                                                ttCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATg                                                               >  1:1578393/1‑149 (MQ=255)
                                                                                gtCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGaa                                                                     <  2:198165/142‑1 (MQ=255)
                                                                                   gTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTg                                                                  >  2:854734/1‑143 (MQ=255)
                                                                                     ttAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGAt                                                                <  2:577664/143‑1 (MQ=255)
                                                                                        aTTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCCCACGCTTNCCTGCAAGGCGTGGCGCTGGAAGCGATGGc                                                             >  2:1263345/1‑143 (MQ=255)
                                                                                         tttCTGAATCTTACCCGGAAGACTGCGGTCTGTTTTCCCCGCTACTGCTGAATGTTTTGAAATTGAACCCTGGCGAAGCGATGTGCCTGTTCGCTTAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTcc                                                      <  1:1676130/149‑1 (MQ=255)
                                                                                          ttCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCg                                                     >  1:361186/1‑149 (MQ=255)
                                                                                             tGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGaaa                                                  >  1:164198/1‑147 (MQ=255)
                                                                                             tGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATa                                                  >  1:615803/1‑149 (MQ=255)
                                                                                              gAATTTTACCCGGACGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTc                                                       <  2:899484/143‑1 (MQ=255)
                                                                                                aTTTTACCCGCAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGACCCCTGGCGACGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTcaa                                                     <  2:465447/143‑3 (MQ=255)
                                                                                                      accggctcacgtctgTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGtgctgc                                         <  1:1379392/135‑1 (MQ=255)
                                                                                                       ccGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCtgcg                                        >  1:489250/1‑149 (MQ=255)
                                                                                                          gAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCtgcgtgc                                     >  1:1712304/1‑149 (MQ=255)
                                                                                                             gACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCtgcg                                        >  2:1519190/1‑143 (MQ=255)
                                                                                                                aGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGGAAACGCCGATAACGTGCTGCGTGCGGGTCt                               <  1:1886253/149‑1 (MQ=255)
                                                                                                                  cGGTCTGTGCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGCTGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCgg                                   <  2:265326/143‑1 (MQ=255)
                                                                                                                    gTCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCGAGGCGTGGCGCTGGAAGTGAGGGCAAACTCCGATAACGTGCTGCGTGCGGGt                                 >  2:1147902/1‑143 (MQ=255)
                                                                                                                     tCTGTTCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGc                          <  1:1550038/149‑1 (MQ=255)
                                                                                                                     tCTGTCCTCCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGc                          <  1:1536173/149‑1 (MQ=255)
                                                                                                                      cTGGTCTCCCCGCTATTGCGGAATGGGTTGAAATTGAACCCTGGCGACGAGCTGTTCCTGTTCGCTGAAACGCCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCt                               <  2:1505909/143‑1 (MQ=255)
                                                                                                                          tctcCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTaaa                     <  1:279492/149‑1 (MQ=255)
                                                                                                                          cctcCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTTCGTGCGGGTCTGACGCCTaaa                     <  1:954114/148‑1 (MQ=255)
                                                                                                                            tcCCCGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGcc                         <  2:526761/143‑1 (MQ=255)
                                                                                                                               ccGCTATTGCTGAATGTGCTGAAATTGAACCCTGGCGAAGCATTTGCTGGTCTCGTGGAAAACCCCCATTCCTGCCTGCAAGGCGTGGCGCTGGAAGTGATGGCCAACTCCGATAACGTGCTGCGTCCCGGTCTGACGCCGAAATACag                >  1:1399303/1‑148 (MQ=255)
                                                                                                                                cGCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGTGTGGCGCTGGAAGTGCTGGCATACTCCGATAACGTGCTGCGTGCGGGGCTGACGCCTAAATACAtt               <  1:1399966/149‑1 (MQ=255)
                                                                                                                                 gCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGCTAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTg              >  1:561059/1‑149 (MQ=255)
                                                                                                                                 gCTATTGCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAAt                    >  2:1353950/1‑143 (MQ=255)
                                                                                                                                       gCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTc        >  1:722129/1‑149 (MQ=255)
                                                                                                                                       gCTGAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTc        >  1:90082/1‑149 (MQ=255)
                                                                                                                                          gAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGata           >  2:1149982/1‑143 (MQ=255)
                                                                                                                                          gAATGTGTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGaga           >  2:1378426/1‑141 (MQ=255)
                                                                                                                                             tgtgTTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGGTGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAAc  >  1:1476484/1‑149 (MQ=255)
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GCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAAC  >  CP009273/1683402‑1683691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: