Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,563,117 G→A H194Y (CAC→TAC)  eutJ ← ethanolamine utilization protein, HSP70/actin superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,563,1170GA99.0% 319.1 / NA 97H194Y (CAC→TAC) eutJethanolamine utilization protein, HSP70/actin superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (43/53);  minor base C (1/0);  total (44/53)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCG  >  CP009273/2562973‑2563262
                                                                                                                                                |                                                                                                                                                 
gAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATACATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTtcaccgtcaccg                                                                                                                                               >  1:465272/1‑149 (MQ=255)
  aaCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCCTTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTAACCGTAACcgcg                                                                                                                                             >  1:360735/1‑149 (MQ=255)
  aaCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACcgcg                                                                                                                                             <  1:1319772/149‑1 (MQ=255)
   aCAGTTCCGCCACGCCCGGTTGCATACAAGGGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCACATCTCTTCACCGTAACCgcgc                                                                                                                                            >  1:1013567/1‑149 (MQ=255)
        tCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCCGGCCAAATCTCTTCCCCGTCACcgcg                                                                                                                                             >  2:567662/1‑143 (MQ=255)
         ccGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCCTGTAc                                                                                                                                      >  1:649809/1‑149 (MQ=255)
           gCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTg                                                                                                                                    >  1:420385/1‑149 (MQ=255)
                 cccGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGc                                                                                                                              <  1:559860/149‑1 (MQ=255)
                 cccGGTGGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGc                                                                                                                              <  1:559767/149‑1 (MQ=255)
                       tGCATACAAGCGCCCCCCGCCAGCCATAAATCAGTAATTCCCTTCCCTTCAATATGCCGGTCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGc                                                                                                                              <  2:143100/143‑1 (MQ=255)
                       tGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTc                                                                                                                        <  1:28962/149‑1 (MQ=255)
                          aTACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAg                                                                                                                     >  1:1259394/1‑149 (MQ=255)
                          aTACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTGGTACTGCTCCGcccc                                                                                                                           >  2:970219/1‑141 (MQ=255)
                           tgcAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGc                                                                                                                    <  1:1346616/147‑1 (MQ=255)
                           tACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGc                                                                                                                    <  1:1205632/149‑1 (MQ=255)
                            aCAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCtt                                                                                                                         <  2:1034878/143‑1 (MQ=255)
                              aaGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATAAGCCGGGCGACAATGTCCGTCATTTCTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGaa                                                                                                                 <  1:1780575/149‑1 (MQ=255)
                               agagCCGCCCGCCAGCCATAAATCAGTAATGCTCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGaaa                                                                                                                <  1:1300561/149‑1 (MQ=255)
                                gagCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAg                                                                                                                     >  2:1192224/1‑143 (MQ=255)
                                  gccgccCGCCAGCCATAAATCAGTAATGCCCTGACCTTTAATAGGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATAc                                                                                                             <  1:1772092/149‑1 (MQ=255)
                                    cgcctgccAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGaa                                                                                                                 <  2:692431/143‑1 (MQ=255)
                                    cgcccgccAGCCATAAATCAGTAATGCCATGAAAAATGGTATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTGGTACTGCTCCGCCTCTTCCAGCgca                                                                                                                 >  2:1702080/1‑141 (MQ=255)
                                      cccgccAGCCATAAATCAGTACTGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAGTCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAGATACGGCg                                                                                                         >  1:954287/1‑149 (MQ=255)
                                      cccgccAGCCATAAATCAGTAATGCCCTGTCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCg                                                                                                         >  1:1162653/1‑149 (MQ=255)
                                      cccgccAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAAt                                                                                                               >  2:375945/1‑143 (MQ=255)
                                      cccgccAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAAt                                                                                                               >  2:1282059/1‑143 (MQ=255)
                                       ccgctAGCCATAAATAATTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGa                                                                                                        <  1:361390/149‑1 (MQ=255)
                                            agccaTAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCg                                                                                                         <  2:116934/143‑1 (MQ=255)
                                             gccaTAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCg                                                                                                  <  1:89863/149‑1 (MQ=255)
                                              ccaTAAATCAGTACTCCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCCGGCCAAATCTCTTCACCAAAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGAt                                                                                                       <  2:423147/143‑1 (MQ=255)
                                              ccaTAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGACCCTTCCAGCGAAATACCGCGAt                                                                                                       >  2:883655/1‑143 (MQ=255)
                                                  aaaTCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCgag                                                                                             <  1:1038506/149‑1 (MQ=255)
                                                   actCAGTAACGCCCTGCCCTTCAATAGGCCGGCCGACAATCTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTCCCAGCGAAATACGGCGATTTCCGGCgaga                                                                                            <  1:335689/147‑1 (MQ=255)
                                                    aTCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTTTTTTTTTTGTTTAAACGCAGGCCAAAACTCTTCACCGCAACCGCGCTTGGACTGCGCCGCGTCCTCCCGCGAAATCCGCAGATTGCCgg                                                                                                 >  2:1721657/1‑143 (MQ=255)
                                                    aTCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATGCGGCGATTTCCGGCGagag                                                                                           >  1:1727942/1‑149 (MQ=255)
                                                    aTCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGCTTTCCgg                                                                                                 >  2:1364595/1‑143 (MQ=255)
                                                       ctgactgccctgcccTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCga                                                                                              <  2:913802/138‑1 (MQ=255)
                                                       aGTAATGCCCTGCCCTTCCATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCa                                                                                        <  1:863039/149‑1 (MQ=255)
                                                         tAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCACCGAAATACGGCGCTTTCCGGCggga                                                                                            >  2:1738636/1‑143 (MQ=255)
                                                         tAATGCCCTGCCCTTCAATATGCCGGGCGACAAAGTCCGCCATTGTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCaga                                                                                      <  1:1012967/149‑1 (MQ=255)
                                                         tAAGGCCCTGCCCTTCAATAAGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTCCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCaga                                                                                      <  1:780799/149‑1 (MQ=255)
                                                            tgccctgcccTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCagagag                                                                                   <  1:1594560/149‑1 (MQ=255)
                                                               cctgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTCCACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCaga                                                                                      <  2:386443/143‑1 (MQ=255)
                                                               cctgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCgga                                                                                      >  2:609964/1‑143 (MQ=255)
                                                               cctgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGGGAgatg                                                                                >  1:1748854/1‑149 (MQ=255)
                                                               cctgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAgatg                                                                                >  1:1545568/1‑149 (MQ=255)
                                                                 tgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCagcga                                                                                    <  2:889073/143‑1 (MQ=255)
                                                                 tgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAgatgtg                                                                              <  1:1733720/149‑1 (MQ=255)
                                                                 tgccctTCAATACGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAgatgtg                                                                              <  1:131324/149‑1 (MQ=255)
                                                                     ctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAgatgtgatgt                                                                          <  1:190188/149‑1 (MQ=255)
                                                                      ttCAATATGCCGGACGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAgatgt                                                                               >  2:1217479/1‑143 (MQ=255)
                                                                       tCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTcc                                                                        <  1:418128/149‑1 (MQ=255)
                                                                         aaTATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGCTCCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTccgc                                                                      <  1:1046122/149‑1 (MQ=255)
                                                                            atGCCGGGCGACAATGTCCCCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGGGCCGGAGAGATCTGATGTCCGCCgg                                                                   >  1:1386196/1‑149 (MQ=255)
                                                                              gCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTg                                                                 <  1:464305/149‑1 (MQ=255)
                                                                               ccGGGTGACAATCGCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTccgc                                                                      <  2:574098/143‑1 (MQ=255)
                                                                                cGGGCGACAATGTCCGCTCTTTGTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGCGGTGATGTccgcc                                                                     >  2:1523013/1‑143 (MQ=255)
                                                                                cGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGc                                                               >  1:8420/1‑149 (MQ=255)
                                                                                 gggCGACACTGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCACATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCt                                                              <  1:905187/149‑1 (MQ=255)
                                                                                     gACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCCGCGAAATACGGCGATTTCCGGCGCGAGTCAGAGAGCTGTCGTGTCCGCCGGTGGCTTCAt                                                          >  1:717041/1‑149 (MQ=255)
                                                                                     gACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGGGCTGGGATGTCCGCCGGTGGCTTcgt                                                          >  1:950851/1‑147 (MQ=255)
                                                                                       cAAGGTCCGCCCTTTTTGCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCCGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCt                                                              <  2:1136170/143‑1 (MQ=255)
                                                                                        aaTGCCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCtt                                                             <  2:1202939/143‑1 (MQ=255)
                                                                                           gTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCAt                                                          <  2:1266759/143‑1 (MQ=255)
                                                                                             ccGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATcc                                                        >  2:1410925/1‑143 (MQ=255)
                                                                                               gCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTATCCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTa                                                <  1:1479274/149‑1 (MQ=255)
                                                                                               gCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATccgc                                                      >  2:963161/1‑143 (MQ=255)
                                                                                                   ttttttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAg                                                  <  2:1758535/143‑1 (MQ=255)
                                                                                                    tttttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCa                                           >  1:1435036/1‑149 (MQ=255)
                                                                                                      tttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACTGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCTGGTGGCTTCATCCGCCGAGTAc                                               <  2:207544/143‑1 (MQ=255)
                                                                                                      tttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGCTTTCCGGCGAGAGTCCGAGAGGGGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTAccggccc                                         >  1:29985/1‑143 (MQ=255)
                                                                                                      tttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTAc                                               <  2:663873/143‑1 (MQ=255)
                                                                                                          gTACACCGGCTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGc                                     <  1:547698/149‑1 (MQ=255)
                                                                                                               ccGGTTTCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTg                                      >  2:1679248/1‑143 (MQ=255)
                                                                                                                    ttCACCGCGGGCCAAATCTCTTCACCGTAACCGCGCTTGTCCTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCtt                                 <  2:87398/143‑1 (MQ=255)
                                                                                                                    ttCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGGACGTCACCTTGCCCtt                                 >  2:1622681/1‑143 (MQ=255)
                                                                                                                    ttCACCGCAGGCCAAATCCCTTCACCGTAACCGCGCGTGTACTGCGCCGCCTCTGCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGAGGTGATGTCCGCCGGTGGTTTCATCCGCCGAGTACGTCACCTTGCCCtt                                 <  2:87404/143‑1 (MQ=255)
                                                                                                                     tCACCGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATCCGGCGCTTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGCGTACGTGCGCTTGCCCgtt                                >  2:161871/1‑143 (MQ=255)
                                                                                                                        ccGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGCGATGTGATCTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTCTTCACgctt                       >  1:1786844/1‑146 (MQ=255)
                                                                                                                        ccGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGCACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATg                       <  1:1065075/149‑1 (MQ=255)
                                                                                                                         cGCAGGCCAACTTCCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGGGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCa                            <  2:52315/143‑1 (MQ=255)
                                                                                                                         cGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTcg                            >  2:1171272/1‑142 (MQ=255)
                                                                                                                         cGCAGGCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATgg                      >  1:234708/1‑149 (MQ=255)
                                                                                                                             ggCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGAt                        <  2:1060574/143‑1 (MQ=255)
                                                                                                                              gCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATg                 >  1:603246/1‑149 (MQ=255)
                                                                                                                              gCCAAATCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGAGGCTTCATCCGCCGAGTGCGTCACCTTGCCCTTTTTCACGATGGCAATg                 >  1:831924/1‑149 (MQ=255)
                                                                                                                                 aaaTCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGCGAGTCAGAGAGATGTGATGTCCGCCGGTGCCTTCACCCGCTGAGTACGTCACCTGGCCCTATTTCACGATGGCa                    >  2:889661/1‑143 (MQ=255)
                                                                                                                                 aaaTCTCTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGCGGCAGAGAGATGTGATGTCCGCCGGTGGCTTCCTCCGCCGAGTACGTCACCTGGCCCTTTTTCCCGCTGGCa                    >  2:523949/1‑143 (MQ=255)
                                                                                                                                    tctcTTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTa           <  1:1067997/149‑1 (MQ=255)
                                                                                                                                     ccctTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAg          <  1:622031/147‑1 (MQ=255)
                                                                                                                                     cccgtCACCGGAACCGCGCTGGTACTGCTCCGCCTCTTCCATCGAAAGACGGCGATTTCCGGCGAGAGTCAGAGAGATGGGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGc                <  2:617118/139‑1 (MQ=255)
                                                                                                                                       ctTCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTg        <  1:23252/149‑1 (MQ=255)
                                                                                                                                        ttCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTgc       >  1:282855/1‑149 (MQ=255)
                                                                                                                                        ttCACCGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGGACGTCACCTTGCCCTTTTTCACGATGGCAATGCCgg             >  2:112544/1‑143 (MQ=255)
                                                                                                                                            ccGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGt         <  2:127747/143‑1 (MQ=255)
                                                                                                                                            ccGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCCGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGt         <  2:1015335/143‑1 (MQ=255)
                                                                                                                                             cGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTgccgccg  >  1:69615/1‑149 (MQ=255)
                                                                                                                                             cGTAACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTg        <  2:64310/143‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTTGCCCTTTTTCACGATGGCAATGCCGGTAGTGCCGCCG  >  CP009273/2562973‑2563262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: