Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,719,4260TC98.4% 388.8 / ‑4.5 129intergenic (‑321/+2)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (68/57);  total (70/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 129 total aligned reads displayed.

ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719280‑2719566
                                                                                                                                                  |                                                                                                                                            
acGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGa                                                                                                                                            <  1:97184/149‑1 (MQ=255)
   tATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGc                                                                                                                                         <  1:1410017/149‑1 (MQ=255)
   tATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGGGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAACGGCCATCCGTGAGGATGGCCTTTCGCATAATTCGATGc                                                                                                                                         >  1:1087415/1‑149 (MQ=255)
     tGGAAGTGGGCAAGTGGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCt                                                                                                                                       <  1:27630/149‑1 (MQ=255)
      ggAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTTTTCTTTTTCAAAAGGGCCATCCGTCAGGATGCCCTTTCGCATAGTTCGa                                                                                                                                            >  2:628204/1‑143 (MQ=255)
      ggAAGTGGGCAAGTGGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGa                                                                                                                                            <  2:318499/143‑1 (MQ=255)
       gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAGGTCGAGCCGTCAGGATGGCCTTTCGCATAATTCGAt                                                                                                                                           <  2:379961/143‑1 (MQ=37)
       gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTgg                                                                                                                                     >  1:1745058/1‑149 (MQ=255)
       gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTgg                                                                                                                                     <  1:1653808/149‑1 (MQ=255)
        aaGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGGCAGGATGGCCTTTCGCATAATTCGATg                                                                                                                                          >  2:1209423/1‑143 (MQ=255)
         aGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCa                                                                                                                                   <  1:1718935/149‑1 (MQ=255)
          gTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGCCAGGATGGCCTTTCGCATAATTCGATGcc                                                                                                                                        >  2:823237/1‑143 (MQ=255)
               ccgtggggggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATACTTCGATGCCTGGCa                                                                                                                                   >  2:1081060/7‑143 (MQ=255)
               cAAGTTGGGGAAGCCGTATCCGGTGCTGTATCGGGAATATGTGGGAGTAGAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCa                                                                                                                                   <  2:187302/143‑1 (MQ=21)
                aaGTTGGGGAAGCCGTATCCGTTACTGAATCTGGAATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCt                                                                                                                            >  1:1053312/1‑149 (MQ=255)
                 aGTTGGGGCAGCCGTCTCCGTCGCTGAATCTGGCATATGTGGGGGTATGAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTa                                                                                                                           <  1:911732/149‑1 (MQ=25)
                        gAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                    >  1:1809563/1‑149 (MQ=255)
                         aaGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCa                                                                                                                   <  1:311644/149‑1 (MQ=255)
                                 gccGGTGCTGAATCTGGGATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                 <  2:639942/142‑1 (MQ=255)
                                  gcGTTGCTGAATCTGGCATGTGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                                <  2:1009936/142‑1 (MQ=255)
                                  ccGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                                <  2:173514/143‑1 (MQ=255)
                                        cTGCATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTGCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAc                                                                                                          <  2:1653844/143‑1 (MQ=255)
                                        cTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                    <  1:1546254/149‑1 (MQ=255)
                                        cTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATGCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                    >  1:614627/1‑149 (MQ=255)
                                         tGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                                                                   >  1:1384281/1‑149 (MQ=255)
                                         tGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                                                                   <  1:259269/149‑1 (MQ=255)
                                           aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCGTAGTCTCGCATGGGGAGAaccc                                                                                                       >  2:898799/1‑143 (MQ=255)
                                           aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                                                                       <  2:824914/143‑1 (MQ=255)
                                           aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa                                                                                                 <  1:1714379/149‑1 (MQ=255)
                                                 gCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATGATTCGATGCCTGGCCGTTCCCTACTCTCGCATGGGGGGACCCCACACTACCATCg                                                                                           >  1:632978/1‑149 (MQ=255)
                                                  cgtatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAc                                                                                                <  2:1382389/141‑1 (MQ=255)
                                                  actatGGGGGAGTATAAGACGCGAAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAc                                                                                                <  2:977020/141‑1 (MQ=255)
                                                   atatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGCTGCCTGGCAGTTCCCTACTCTCGCATGGGGCGACCCCACCCTCCCCTCGgc                                                                                         >  1:733151/1‑149 (MQ=25)
                                                      tgtgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCt                                                                                      >  1:49343/1‑149 (MQ=255)
                                                        tgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAc                                                                                    <  1:1272695/149‑1 (MQ=255)
                                                         gggAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACg                                                                                   >  1:1629376/1‑149 (MQ=255)
                                                          tgAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcg                                                                                        <  2:612486/142‑1 (MQ=255)
                                                          tgAGTATAAGACGCGCAGCCTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcg                                                                                        <  2:612405/142‑1 (MQ=255)
                                                          ggAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGACCCCAGACTACCCTCGGCGCTACgg                                                                                  >  1:89142/1‑149 (MQ=255)
                                                          ggAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACgg                                                                                  >  1:18484/1‑149 (MQ=255)
                                                              tataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCACTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCACTACTCTCGCATGGGGAGACCCCCCACTACCACCGGCGCTAc                                                                                    >  2:42197/1‑143 (MQ=21)
                                                              tataAGAAGCGGAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTCCCATCGGCGCTAc                                                                                    >  2:1073286/1‑143 (MQ=255)
                                                               ttaAGACGCGCGTGGGCGCATCAGGCATTTTTTTCTGCGCAAATGCAAAAAGGCTATCCGTCAGGATGGCCTGTCGCATAATTCGCTGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACg                                                                                   <  2:800334/142‑1 (MQ=25)
                                                               ataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCCGGGGGGGACCCCACACTACCATCGGCGCTACGGCGttt                                                                             >  1:1279168/1‑149 (MQ=255)
                                                               ataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGGGACCCCACACTACCATCGGCGCTGCGGCGttt                                                                             >  1:1111280/1‑149 (MQ=255)
                                                                taAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGAAGACCCCACACTCCCCTCGGCGCTACgg                                                                                  >  2:225912/1‑143 (MQ=255)
                                                                 aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGc                                                                                 >  2:1233232/1‑143 (MQ=255)
                                                                 aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                                                           >  1:302383/1‑149 (MQ=255)
                                                                 aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                                                           >  1:1671383/1‑149 (MQ=255)
                                                                 aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                                                           <  1:415309/149‑1 (MQ=255)
                                                                   gACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGt                                                                               >  2:1657864/1‑143 (MQ=255)
                                                                      gcgcAGCGTCGCATCAGGCATTTTTGTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTCCGGCGTTTCACTTCt                                                                      >  1:960754/1‑149 (MQ=255)
                                                                      gcgcAGCGTCGCATCAGGCATTTTTGTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCt                                                                      >  1:959965/1‑149 (MQ=255)
                                                                        gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                    <  1:1017427/149‑1 (MQ=255)
                                                                        ccAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAc                                                                          <  2:172806/142‑1 (MQ=255)
                                                                           ggctCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATCCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                                                                 <  1:921422/146‑1 (MQ=255)
                                                                            cGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCt                                                                      <  2:1235624/143‑1 (MQ=255)
                                                                                       gCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGCCCCCCCACTACCACCGGCGCTCCGGCGCTTCAGTCCTGAGTTCGGCaa                                                           >  2:1499119/1‑142 (MQ=21)
                                                                                       gCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTTGGGTc                                                     >  1:157839/1‑149 (MQ=255)
                                                                                       gCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAt                                                           >  2:905385/1‑143 (MQ=255)
                                                                                       gCATTTTTTTCTGCGCCAATGCAAAAAGGCCATAAGATAAAATGTGCGTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTc                                                     >  1:1881737/1‑149 (MQ=17)
                                                                                       gCATTGTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTGCGCATAATTGGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGCGTTCGGCAt                                                           <  2:1624599/143‑1 (MQ=255)
                                                                                             ttttCTTCTCCATTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCCCTTCTGAGTTCGGCCTGGGGTc                                                     >  2:983472/1‑143 (MQ=18)
                                                                                             ttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                               <  1:573048/149‑1 (MQ=255)
                                                                                                 cTGCGCCAATGCAAAAAGGCCATCCGGCGGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGAGacc                                           >  1:1023900/1‑149 (MQ=18)
                                                                                                   gcgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                               >  2:81276/1‑143 (MQ=255)
                                                                                                   gcgcCAATGCAAAAAGGCCATCCGTCAGAATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccac                                         >  1:1019435/1‑149 (MQ=255)
                                                                                                    cgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc                                        >  1:728134/1‑149 (MQ=255)
                                                                                                     gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTgggg                                             >  2:9693/1‑142 (MQ=255)
                                                                                                      ccgctGCAAAAAGGCCATCCGTGAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                            <  2:304927/139‑1 (MQ=255)
                                                                                                       cAATGCAAAAAGGCCATCCGTCAGGATGGCCTGTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg                                     <  1:1318540/149‑1 (MQ=255)
                                                                                                        aaTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCCGTTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGacca                                          <  2:1734285/143‑1 (MQ=255)
                                                                                                            cAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTGCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGGATGGGGCCAGGTGGACCCACCGCGCTCCg                                >  1:1790760/1‑149 (MQ=17)
                                                                                                            cAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                      >  2:119585/1‑143 (MQ=255)
                                                                                                            cAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACg                                <  1:1721090/149‑1 (MQ=255)
                                                                                                            cAAAAAGGCCATCCAAGGGGGGGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                      >  2:1071615/1‑143 (MQ=14)
                                                                                                             aaaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACgg                               >  1:858023/1‑149 (MQ=255)
                                                                                                              gaaaGGCCATCCTGACGGAGGACCATTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCgcgc                                    >  2:1750430/2‑143 (MQ=11)
                                                                                                              aaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCAGCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgc                              <  1:890198/149‑1 (MQ=255)
                                                                                                                taGGCCATCCGTCAGGATGGCCTTTCGCTTAATTCGTTGCCTGGTTGTTCCTTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccg                            <  1:691316/148‑1 (MQ=14)
                                                                                                                aaggccatcctgacggATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGCGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACGgccg                            >  1:537142/15‑149 (MQ=14)
                                                                                                                 aGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccgc                           <  1:1282159/149‑1 (MQ=255)
                                                                                                                      aTCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGc                      >  1:1539920/1‑149 (MQ=255)
                                                                                                                       cccGGCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccgc                           <  2:560684/142‑1 (MQ=255)
                                                                                                                        ccGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaa                    <  1:330626/149‑1 (MQ=255)
                                                                                                                          tgcgccttGGCCGTTTGCTTGATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                        <  2:47037/136‑1 (MQ=1)
                                                                                                                          gTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                        >  2:1769946/1‑143 (MQ=255)
                                                                                                                           tCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAgg                       >  2:1677366/1‑143 (MQ=255)
                                                                                                                              ggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaa                    <  2:989770/143‑1 (MQ=255)
                                                                                                                               gATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaaa                   >  2:311928/1‑143 (MQ=255)
                                                                                                                               gATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt             <  1:1343171/149‑1 (MQ=255)
                                                                                                                                  ggCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGtttt          <  1:841698/149‑1 (MQ=38)
                                                                                                                                    ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGTCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGTGGCCGCCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        >  1:1712957/1‑149 (MQ=11)
                                                                                                                                    ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        <  1:1788029/149‑1 (MQ=37)
                                                                                                                                    ccTTTCGCATAATTCGATGCCTGGCAGTTCACTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCCCCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        >  1:996982/1‑149 (MQ=255)
                                                                                                                                     cTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCACATTCTGTTTTATc       >  1:597546/1‑149 (MQ=255)
                                                                                                                                      tttCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCACACACTACCATCGGCGCTACGGCGTGTGACTTCTGAGTTCGGCATGGGGTCAGGTGGGACGACCGCGCTACGGCCGCCACGCAAATTCTGtt            >  2:425004/1‑143 (MQ=11)
                                                                                                                                          gCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTaagatcgg  >  1:1069497/1‑142 (MQ=12)
                                                                                                                                           cATAATTCGATGCCTGGCGGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCCAAATCAGTTTTATc       >  2:642775/1‑143 (MQ=11)
                                                                                                                                           cATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATc       >  2:724745/1‑143 (MQ=14)
                                                                                                                                                  |                                                                                                                                            
ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719280‑2719566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: