Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,960,102 G→T R109L (CGC→CTC)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,960,1020GT96.8% 280.4 / ‑6.6 97R109L (CGC→CTC) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base G (2/0);  major base T (50/41);  minor base C (3/0);  total (56/41)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAA  >  CP009273/3959958‑3960241
                                                                                                                                                |                                                                                                                                           
gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGACCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGGGAActctgt                                                                                                                                         >  1:1497292/1‑147 (MQ=255)
 atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAtt                                                                                                                                        <  1:41166/149‑1 (MQ=255)
    tGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTg                                                                                                                                     <  1:1452200/149‑1 (MQ=255)
    tGGCTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTg                                                                                                                                     <  1:1902908/149‑1 (MQ=255)
      gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTgcg                                                                                                                                   <  1:579851/149‑1 (MQ=255)
      gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTCTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAt                                                                                                                                         >  2:561126/1‑143 (MQ=255)
       tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCTGCCCCCGCGCTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAtt                                                                                                                                        >  2:1055071/1‑143 (MQ=255)
       tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTgccc                                                                                                                                  >  1:1441444/1‑147 (MQ=255)
       tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCCCTGGTAAGATTCGCCCGCCGAAAGAAGGGGAACTCTATTTTgcgc                                                                                                                                  >  1:1320059/1‑149 (MQ=255)
       tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACGGGGGATACCATCGCTGGTAGGATTCGCCCGCCGAAAGAAGGGGAACTCTATTTGgcgc                                                                                                                                  >  1:1320386/1‑149 (MQ=255)
          cctccGTTCCGCAGACAGCTCCGCCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAGCTCTATTTTGCgctgc                                                                                                                               <  1:1208604/149‑1 (MQ=255)
           ctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGGCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTgc                                                                                                                                    >  2:865256/1‑143 (MQ=255)
            tccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGGGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGACGGTGAACCCTATTTTGCGctgctg                                                                                                                             >  1:136793/1‑149 (MQ=255)
             ccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTgcgc                                                                                                                                  >  2:1234272/1‑143 (MQ=255)
             ccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGa                                                                                                                            >  1:1385991/1‑149 (MQ=255)
             ccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTGgcgc                                                                                                                                  >  2:318928/1‑143 (MQ=255)
              cGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTCTCAACCTCCGCACTGGTGCTACCATCTCTGGTAACATTCGCCCGCCGAAAGAAGGTGAACCATCTTTTCCgct                                                                                                                                 >  2:1144386/1‑143 (MQ=255)
                ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAg                                                                                                                         <  1:1668476/149‑1 (MQ=255)
                  ccGCAGACAGCCCCTACCTCGCCAGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATTCCATCTCTGGTAGGATTCGCCCGCCGAAAGAAGGTGAATGCTATTTTACgatgcag                                                                                                                             >  2:1202942/1‑141 (MQ=255)
                   cGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACCCTATTTTGCGCTGCTGa                                                                                                                            >  2:306959/1‑143 (MQ=255)
                         cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTa                                                                                                                      >  2:1651954/1‑143 (MQ=255)
                             tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTaa                                                                                                            >  1:1097354/1‑149 (MQ=255)
                             tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCATAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGCTaa                                                                                                            >  1:73355/1‑149 (MQ=255)
                                  ccTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTACCGAAGTTAACTTCg                                                                                                       >  1:693996/1‑149 (MQ=255)
                                   cTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGa                                                                                                      <  1:542456/149‑1 (MQ=255)
                                   cTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACACTATTTTGCGCTGCTGCAAGTTAACGAAGTTaa                                                                                                            >  2:783039/1‑143 (MQ=255)
                                    ccGCCGGTCCTGATGACATCTACGTTTCCCCGAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAc                                                                                                     <  1:502101/148‑1 (MQ=255)
                                     cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTATGGTAAGGTTCGCCCGACAAATTTTGGTGGACTCTATTTTGCGCTGCTGAAAGTTACCGAAGTTAAATTCGACa                                                                                                    >  1:838269/1‑149 (MQ=255)
                                      gCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACAGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGGGAACTCTATTTTGCGCGGCTGAAAGTTAACGAAGTTAACTTccccaa                                                                                                   >  1:1268154/1‑144 (MQ=255)
                                               gatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaaa                                                                                          >  1:1151684/1‑149 (MQ=255)
                                                atgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTGTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGCCAAAccg                                                                                               >  2:739148/1‑142 (MQ=255)
                                                  gaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGc                                                                                       <  1:264139/149‑1 (MQ=255)
                                                    cATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                                                                           <  2:1328634/143‑1 (MQ=255)
                                                     aTCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAAATCTATTTTTCGCTGCTGACAGTTAACGAAGTTAACTTCGCAAAACCTTAAAACGcccc                                                                                    >  1:423425/1‑148 (MQ=255)
                                                     aTCTACGTTTCCCCTAGCCAAATCCGCCGATTCAACATCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCACCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGCAAGTTAACGAAGTTAACTTCGACAAACCTGaaaa                                                                                          >  2:66097/1‑143 (MQ=255)
                                                      tCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAAc                                                                                         <  2:124295/143‑1 (MQ=255)
                                                      tCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAAc                                                                                         <  2:1220755/143‑1 (MQ=255)
                                                        tccGTTTCCCCTAGCCAAATCCGCCGTTTCAACCGCCGCCCTGGTGATACCATCTCTGGTAAGCTTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGc                                                                                       <  2:1377853/141‑1 (MQ=255)
                                                          cGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaaca                                                                               >  1:1089609/1‑149 (MQ=255)
                                                            tttCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCGCTGGTGATACCATCTCTGGTGAGATTCGCCCGCGGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGCAGTTAACTGCGACCAACCTGAAAGCGCCCGcacgaga                                                                             >  1:552496/1‑144 (MQ=255)
                                                            tttCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTCACTTCGACAAACCGGaaacaccccgc                                                                                   >  2:1577067/1‑135 (MQ=255)
                                                            tttCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCACGCAACaaa                                                                             >  1:258370/1‑149 (MQ=255)
                                                               ccccTAGCCAAATCCCACGTATAAGCCTTCTCTCTGGTGATAACTGCAGGAATAATATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc                                                                          <  1:1857811/149‑1 (MQ=255)
                                                                cccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGCAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCCGAAAACGCCCGcaaca                                                                               >  2:1449010/1‑143 (MQ=255)
                                                                  cTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaa                                                                             >  2:1530017/1‑143 (MQ=255)
                                                                     gCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTGCGACAAACCTGAAAACGCCCGCAACAAAATc                                                                          <  2:689104/143‑1 (MQ=255)
                                                                     cccAAATCCGCCGTTTCAACCTCCGCACTGGTCATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCttt                                                                    <  1:313947/148‑1 (MQ=255)
                                                                      ccAAATCCGCCGTTTCAACCTCCGCACTGGTGCTACTGTCTCAGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAATTTCGACCAACCTGAAAACGCCCGCAACGAAATCCCCTTTg                                                                   >  1:934519/1‑149 (MQ=255)
                                                                      ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACGAAGTCCTCGTTg                                                                   >  1:1663270/1‑149 (MQ=255)
                                                                      ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg                                                                   <  1:379999/149‑1 (MQ=255)
                                                                       cAAACCAGCCGTTTCAACCGCCGCGCTGGAGATGCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGCGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTga                                                                  <  1:73714/149‑1 (MQ=255)
                                                                         aaTGCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGCTTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCCGAAAACGCCCGCAACAAAATCCTCTTTgaga                                                                <  1:697376/149‑1 (MQ=255)
                                                                             cgccgTTTCGACCTCCGCACTGGTGATCCCATCTCTGCTAAGATTCTCCCGCAGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAACCCTCTTTga                                                                  <  2:751043/143‑1 (MQ=255)
                                                                              gccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTa                                                           <  1:96756/149‑1 (MQ=255)
                                                                              gccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTa                                                           >  1:656472/1‑149 (MQ=255)
                                                                                  tttCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTCAACTTCGACGAACCTGCAAACGCCCGCAAGAAAATCCTGTTTGAGAACTTGAcgc                                                       >  1:758737/1‑147 (MQ=255)
                                                                                      accccccccACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAATTCCTCTTTGAGAACTTAACCCCGCt                                                   <  1:1139703/141‑1 (MQ=255)
                                                                                      aaCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCCCt                                                   >  1:1110829/1‑149 (MQ=255)
                                                                                       aCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc                                                        <  2:1180018/143‑1 (MQ=255)
                                                                                          tCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc                                               <  1:1088732/149‑1 (MQ=255)
                                                                                            cGCACTGGTGATACCATCTCTGGTAAGATTCGCTCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt                                                   <  2:8614/143‑1 (MQ=255)
                                                                                            cGCACTGGTGAGACCATCTCTGGTAAGATTCGGCCGCCGAAAGAAGGTGAACTCTATTGTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt                                                   <  2:329963/143‑1 (MQ=255)
                                                                                              cACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa                                           <  1:215582/149‑1 (MQ=255)
                                                                                               aCTGGGGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaaa                                          <  1:615092/149‑1 (MQ=255)
                                                                                                   gTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                                            >  2:75567/1‑143 (MQ=255)
                                                                                                   gTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                                            >  2:526496/1‑143 (MQ=255)
                                                                                                   gTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                                            >  2:1585009/1‑143 (MQ=255)
                                                                                                   gTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGAGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActct                                      >  1:845537/1‑149 (MQ=255)
                                                                                                    tGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGTGAACTTAACCCCGCTGCACGCAAACtctc                                     <  1:1344248/149‑1 (MQ=255)
                                                                                                     gATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaaa                                          >  2:743361/1‑143 (MQ=255)
                                                                                                      aTACCATCTCTGGTAAGATTAGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACATGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCGGGTGCACGCAAACTCTCGt                                   >  1:1066802/1‑149 (MQ=255)
                                                                                                      aTACAATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTAAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGGACTTAACCCCGCTGCACGCAAAc                                         >  2:104522/1‑143 (MQ=255)
                                                                                                        aCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGGACGCAAGCTCTGGTCt                                 >  1:1586279/1‑149 (MQ=255)
                                                                                                        aCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                                 >  1:523692/1‑149 (MQ=255)
                                                                                                        aCCATCTCTGGTAAGAGTCGCCCGCCGAAAGAAAGTGGACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAGCCCCGCTGCACGCAAACTCTCGTCt                                 >  1:1216292/1‑149 (MQ=255)
                                                                                                           aTCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTTTTTTTTTTTTTGTTAACGAAGTGACCTTCGACAAACCTGAAGACGCCCGCAACAGAATCCTCTTTGAGACCTTAACCCCCCTGCCCGCAAACtctcc                                    >  2:669739/1‑142 (MQ=255)
                                                                                                            tctcTGGTAAGATTCGCGCGACGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTTACGAAGTTAACTTCGACAAGACTGAAAACGCACGCAACAAGCTCTTCTTTGAGAAGTTAACCCCGCTGCACGCAAACTCTCGt                                   <  2:1749727/143‑1 (MQ=255)
                                                                                                              tctGGTAAGATTCGCCCGCCGAAAGAAGGTGAAGTCTATTTTGCGGTGCTGAAAGTTACCCACGATATTGGCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAACCTCTCGTCTGCGTAt                           >  1:1783052/1‑149 (MQ=255)
                                                                                                              tctGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGACGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCATCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTAt                           <  1:1823622/149‑1 (MQ=255)
                                                                                                              tctGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                                 >  2:941056/1‑143 (MQ=255)
                                                                                                               ctGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTCTACGCAGACTATCGTCTGCgcagg                          >  1:1854735/1‑145 (MQ=255)
                                                                                                                tGGTAATATGCGTCCGCGGAAAGAAGGTGAATTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTATCTTCGACAAACCTGAAAACGCCCGCACCAAAATCCGCTTATAGTATTCAACCCTGCTGCACGCAAACTCTCGTCTGc                               <  2:805149/143‑1 (MQ=255)
                                                                                                                   tAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc                      <  1:97216/149‑1 (MQ=255)
                                                                                                                     aGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt                    >  1:1365645/1‑149 (MQ=255)
                                                                                                                      gATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg                   >  1:1739090/1‑149 (MQ=255)
                                                                                                                            cccgccGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACCAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg                   <  2:896614/143‑1 (MQ=255)
                                                                                                                            cccgccGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg                   >  2:1588656/1‑143 (MQ=255)
                                                                                                                               gccgAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAATTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTa                <  2:1099388/143‑1 (MQ=255)
                                                                                                                                ccgAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTc         >  1:1633795/1‑149 (MQ=255)
                                                                                                                                   aaaGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg            >  2:1371365/1‑143 (MQ=255)
                                                                                                                                     agaagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTTGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCTCGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt          <  2:1739100/143‑1 (MQ=255)
                                                                                                                                     agaagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt          <  2:1221916/143‑1 (MQ=255)
                                                                                                                                     agaagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTg    >  1:823692/1‑149 (MQ=255)
                                                                                                                                      gaagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGa   <  1:417960/149‑1 (MQ=255)
                                                                                                                                       aagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGaa  >  1:1566686/1‑149 (MQ=255)
                                                                                                                                        agGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTa       <  2:991894/143‑1 (MQ=255)
                                                                                                                                           tGAACTCTATTTTGCGCTGCTGGAAGTTAACGAAGTTACCTACGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTg    <  2:782256/143‑1 (MQ=255)
                                                                                                                                             aaCTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGaa  <  2:816387/143‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAA  >  CP009273/3959958‑3960241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑