Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,191,676 C→T H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,191,6760CT97.9% 101.0 / NA 47H366H (CAC→CATicde14 prophage; isocitrate dehydrogenase, specific for NADP+
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/18);  minor base G (0/1);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGA  >  CP009273/1191534‑1191798
                                                                                                                                              |                                                                                                                           
gtatcggtatcgCCCCTGGTGCAAACATCGGGGACGAATGCGCCCTGTTTGAAGCCAGCCACGGTACTGCGCCGAAAGATCGCGGTCAGGACAAAGTGAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGt                                                                                                                       <  2:84227/149‑1 (MQ=255)
  ctcggtacagCCACTGGTGCAAACAGCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTg                                                                                                                     <  2:900648/148‑1 (MQ=255)
  atcggtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTg                                                                                                                     >  1:735392/1‑149 (MQ=255)
   tcggtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGCAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTCTTATTCTCTCCGCGGAGATGATTCTGGGCCATATGGGGTgg                                                                                                                    >  2:658961/1‑149 (MQ=255)
    cggtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCCTGTGGGTTGGa                                                                                                                   >  1:347551/1‑149 (MQ=255)
              ccTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGAACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGc                                                                                                        >  1:187497/1‑149 (MQ=255)
                  gTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTGTCCGCTGAGATGATGCTGCGCCATATGGGTTGGGCTGAAGCGGCTGAc                                                                                                    >  1:13585/1‑149 (MQ=255)
                  gTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATCTGGGTTGGACTGAAGCGGCTggc                                                                                                    >  1:116289/1‑147 (MQ=255)
                      aaaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgg                                                                                                >  2:63937/1‑145 (MQ=255)
                       aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgat                                                                                               >  1:852974/1‑144 (MQ=255)
                            cGGTGACGAATGCGCCATGTTTGAAGCCAGCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAAATGATGCTGCGCGCTATGGGTTGGACTGAAGCGGTTGACCTGATTGTTa                                                                                          >  1:599854/1‑149 (MQ=255)
                              gTGACGAATTCGCCCTGTTTGAAGCCCCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTCTTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                                                        <  2:108225/149‑1 (MQ=37)
                               tGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGGCCTGATTGTTAAAg                                                                                       >  2:192470/1‑149 (MQ=38)
                                       gcgcCCAGATTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGACAGGACAAAGTAAATCCTGGATCTAATATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                                                                               <  1:61049/149‑1 (MQ=25)
                                        cgcCCTGTCTGGAGCCACCCACGGTACCGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAg                                                                              <  2:63526/149‑1 (MQ=37)
                                         gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATGGTTAAAGGGATGGAAgg                                                                             >  1:771946/1‑149 (MQ=37)
                                         gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATACTGGCGCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGGGTGGGAgg                                                                             >  1:796700/1‑149 (MQ=37)
                                           ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                           >  1:473988/1‑149 (MQ=255)
                                           ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                           <  1:956844/149‑1 (MQ=37)
                                           ccTGTTTAAAGCCACCCACGTTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                           <  1:782658/149‑1 (MQ=25)
                                              gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTGTGGAAGGCGCaa                                                                        >  1:416728/1‑149 (MQ=37)
                                                  gAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa                                                                    <  1:98649/149‑1 (MQ=25)
                                                     gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                                                 <  1:731164/149‑1 (MQ=25)
                                                       cccccacGGCACTGCGCCGCAATATGCCGGTCAGCACAAAGTAAATCCTGGCTCTATTATTCTCTCCATGGAGATGATGCTGTGCCAGTGTTGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgcca                                                               <  1:941921/147‑6 (MQ=18)
                                                        acccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa                                                              >  2:543614/1‑149 (MQ=21)
                                                         cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaag                                                             >  1:795915/1‑142 (MQ=21)
                                                               gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGGTAAAGGTATGGGAGGCGCAATCAATGCCAAGACCGTa                                                       >  1:273059/1‑149 (MQ=18)
                                                                    gcgcCGAAAGAGGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCGGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa                                                  <  2:46792/149‑1 (MQ=17)
                                                                     cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGGAGGCGCAATCAGTGCCAAGAACGTAActttt                                                 >  1:171861/1‑145 (MQ=18)
                                                                     cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACCGACGAGGAATGGCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                                                 <  1:506064/149‑1 (MQ=18)
                                                                       ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTTACTTGAATGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                                               <  1:31977/149‑1 (MQ=18)
                                                                        cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGGTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAc                                              >  1:729438/1‑149 (MQ=17)
                                                                          aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                            >  1:14217/1‑149 (MQ=17)
                                                                          aaaTAGGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                            <  2:41421/149‑1 (MQ=17)
                                                                                gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACg                                      >  1:813580/1‑149 (MQ=14)
                                                                                  cGGTCAGGACAAAGTAAATCCTGGCTCTTTTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                    <  2:16573/149‑1 (MQ=14)
                                                                                  cGGGCAGGACATAGTGAATCCTGGCTCTTTTATGCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                    <  2:468857/149‑1 (MQ=11)
                                                                                    gTCAGGACAAAGTAAATCCTGTCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                  >  2:801840/1‑149 (MQ=14)
                                                                                    gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGGGATGATGCTGCGCCATATGGGTTGGACTGAATCGGCTGGCCTGGCTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                  <  2:751202/149‑1 (MQ=14)
                                                                                         gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatgga                             >  2:16514/1‑149 (MQ=14)
                                                                                          aCAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatggga                            >  1:400441/1‑147 (MQ=11)
                                                                                                         gCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg             <  2:544749/149‑1 (MQ=14)
                                                                                                             tattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCATGACCGTAACTTATGACTTTGAACGCCTGATGGAAGGCGCTAAGCTGCTGAAAt         <  1:525884/149‑1 (MQ=14)
                                                                                                             tattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt         >  1:927107/1‑149 (MQ=14)
                                                                                                             tattatTCTCTCAGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt         >  2:800875/1‑149 (MQ=14)
                                                                                                              attattCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACAGTAACTTATGAATTCGAAGGTCTGATGGAAGGCCCTAAGCTGCTGAAATg        >  2:849119/1‑149 (MQ=14)
                                                                                                                    ctctcCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGACGCTAAGCTGCTGAACTGTTCaga  >  2:120041/1‑149 (MQ=14)
                                                                                                                                              |                                                                                                                           
GTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGA  >  CP009273/1191534‑1191798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: