Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,191,703	A→G	L375M (TTA→CTG)	icd →	e14 prophage; isocitrate dehydrogenase, specific for NADP+

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,191,703	0	A	G	100.0%	83.3 / NA	37	L375L (TTA→TTG)	icd	e14 prophage; isocitrate dehydrogenase, specific for NADP+
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/17);  total (20/17)

CGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGG‑CTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGA  >  CP009273/1191562‑1191798                                                                                                                                               |                                                                                                cGGTGACGAATGCGCCATGTTTGAAGCCAGCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAAATGATGCTGCGCGCTATGGGTTGGACTGAAGCGG‑TTGACCTGATTGTTa                                                                                          >  1:599854/1‑149 (MQ=255)   gTGACGAATTCGCCCTGTTTGAAGCCCCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTCTTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTaaa                                                                                        <  2:108225/149‑1 (MQ=37)    tGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGGCCTGATTGTTAAAg                                                                                       >  2:192470/1‑149 (MQ=38)            gcgcCCAGATTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGACAGGACAAAGTAAATCCTGGATCTAATATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGaa                                                                               <  1:61049/149‑1 (MQ=25)             cgcCCTGTCTGGAGCCACCCACGGTACCGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAg                                                                              <  2:63526/149‑1 (MQ=37)              gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATGGTTAAAGGGATGGAAgg                                                                             >  1:771946/1‑149 (MQ=37)              gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATACTGGCGCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGGGTGGGAgg                                                                             >  1:796700/1‑149 (MQ=37)                ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                           >  1:473988/1‑149 (MQ=255)                ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                           <  1:956844/149‑1 (MQ=37)                ccTGTTTAAAGCCACCCACGTTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                           <  1:782658/149‑1 (MQ=25)                   gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTGTGGAAGGCGCaa                                                                        >  1:416728/1‑149 (MQ=37)                       gAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa                                                                    <  1:98649/149‑1 (MQ=25)                          gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                                                 <  1:731164/149‑1 (MQ=25)                            cccccacGGCACTGCGCCGCAATATGCCGGTCAGCACAAAGTAAATCCTGGCTCTATTATTCTCTCCATGGAGATGATGCTGTGCCAGTGTTGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgcca                                                               <  1:941921/147‑6 (MQ=18)                             acccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa                                                              >  2:543614/1‑149 (MQ=21)                              cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaag                                                             >  1:795915/1‑142 (MQ=21)                                    gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGGTAAAGGTATGGGAGGCGCAATCAATGCCAAGACCGTa                                                       >  1:273059/1‑149 (MQ=18)                                         gcgcCGAAAGAGGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCGGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa                                                  <  2:46792/149‑1 (MQ=17)                                          cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGGAGGCGCAATCAGTGCCAAGAACGTAActttt                                                 >  1:171861/1‑145 (MQ=18)                                          cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACCGACGAGGAATGGC‑TGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                                                 <  1:506064/149‑1 (MQ=18)                                            ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTTACTTGAATGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                                               <  1:31977/149‑1 (MQ=18)                                             cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGGTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAc                                              >  1:729438/1‑149 (MQ=17)                                               aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                            >  1:14217/1‑149 (MQ=17)                                               aaaTAGGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                            <  2:41421/149‑1 (MQ=17)                                                     gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACg                                      >  1:813580/1‑149 (MQ=14)                                                       cGGTCAGGACAAAGTAAATCCTGGCTCTTTTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                    <  2:16573/149‑1 (MQ=14)                                                       cGGGCAGGACATAGTGAATCCTGGCTCTTTTATGCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                    <  2:468857/149‑1 (MQ=11)                                                         gTCAGGACAAAGTAAATCCTGTCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                  >  2:801840/1‑149 (MQ=14)                                                         gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGGGATGATGCTGCGCCATATGGGTTGGACTGAATCGG‑CTGGCCTGGCTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                  <  2:751202/149‑1 (MQ=14)                                                              gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatgga                             >  2:16514/1‑149 (MQ=14)                                                               aCAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatggga                            >  1:400441/1‑147 (MQ=11)                                                                              gCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg             <  2:544749/149‑1 (MQ=14)                                                                                  tattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCATGACCGTAACTTATGACTTTGAACGCCTGATGGAAGGCGCTAAGCTGCTGAAAt         <  1:525884/149‑1 (MQ=14)                                                                                  tattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt         >  1:927107/1‑149 (MQ=14)                                                                                  tattatTCTCTCAGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt         >  2:800875/1‑149 (MQ=14)                                                                                   attattCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACAGTAACTTATGAATTCGAAGGTCTGATGGAAGGCCCTAAGCTGCTGAAATg        >  2:849119/1‑149 (MQ=14)                                                                                         ctctcCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGACGCTAAGCTGCTGAACTGTTCaga  >  2:120041/1‑149 (MQ=14)                                                                                                                                               |                                                                                                CGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGG‑CTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGA  >  CP009273/1191562‑1191798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑