Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,191,739	A→G	K387K (AAA→AAG)	icd →	e14 prophage; isocitrate dehydrogenase, specific for NADP+

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,191,739	0	A	G	100.0%	37.1 / NA	21	K387K (AAA→AAG)	icd	e14 prophage; isocitrate dehydrogenase, specific for NADP+
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/9);  total (12/9)

GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGG‑CTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGA  >  CP009273/1191597‑1191798                                                                                                                                                |                                                            gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGGTAAAGGTATGGGAGGCGCAATCAATGCCAAGACCGTa                                                       >  1:273059/1‑149 (MQ=18)      gcgcCGAAAGAGGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCGGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa                                                  <  2:46792/149‑1 (MQ=17)       cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGGAGGCGCAATCAGTGCCAAGAACGTAActttt                                                 >  1:171861/1‑145 (MQ=18)       cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACCGACGAGGAATGGC‑TGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                                                 <  1:506064/149‑1 (MQ=18)         ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTTACTTGAATGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                                               <  1:31977/149‑1 (MQ=18)          cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGGTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAc                                              >  1:729438/1‑149 (MQ=17)            aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                            >  1:14217/1‑149 (MQ=17)            aaaTAGGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                            <  2:41421/149‑1 (MQ=17)                  gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACg                                      >  1:813580/1‑149 (MQ=14)                    cGGTCAGGACAAAGTAAATCCTGGCTCTTTTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                    <  2:16573/149‑1 (MQ=14)                    cGGGCAGGACATAGTGAATCCTGGCTCTTTTATGCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                    <  2:468857/149‑1 (MQ=11)                      gTCAGGACAAAGTAAATCCTGTCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                  >  2:801840/1‑149 (MQ=14)                      gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGGGATGATGCTGCGCCATATGGGTTGGACTGAATCGG‑CTGGCCTGGCTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                  <  2:751202/149‑1 (MQ=14)                           gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatgga                             >  2:16514/1‑149 (MQ=14)                            aCAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatggga                            >  1:400441/1‑147 (MQ=11)                                           gCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg             <  2:544749/149‑1 (MQ=14)                                               tattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCATGACCGTAACTTATGACTTTGAACGCCTGATGGAAGGCGCTAAGCTGCTGAAAt         <  1:525884/149‑1 (MQ=14)                                               tattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt         >  1:927107/1‑149 (MQ=14)                                               tattatTCTCTCAGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt         >  2:800875/1‑149 (MQ=14)                                                attattCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACAGTAACTTATGAATTCGAAGGTCTGATGGAAGGCCCTAAGCTGCTGAAATg        >  2:849119/1‑149 (MQ=14)                                                      ctctcCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGACGCTAAGCTGCTGAACTGTTCaga  >  2:120041/1‑149 (MQ=14)                                                                                                                                                |                                                            GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGG‑CTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGA  >  CP009273/1191597‑1191798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑