Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	445,409	C→T	W230* (TGG→TAG)	cyoB ←	cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	445,409	0	C	T	100.0%	139.8 / NA	50	W230* (TGG→TAG)	cyoB	cytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/24);  total (26/24)

GTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACT  >  CP009273/445267‑445554                                                                                                                                               |                                                                                                                                                  gTTGATGTACATCATCATGTTTCCACCCATATCGTTGGTAAAGAAATGGGTGCGCAGAAAGGGAGCGAGGGTAAACAACGCGACGGTGACCGGCAGAAGTGGGGAGGAAGCAATAATCAGGACGTTTGCGCACAGGGATGCATAGGTaa                                                                                                                                             >  1:32810/1‑149 (MQ=255) gTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGCTCAACAACGCGACGGTAACCGACCGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGATaa                                                                                                                                             >  1:329057/1‑149 (MQ=255)   tGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATGCAGGGGCAACAACGCGACGGTAACCGTCGGAATTGGGAAAGAAGCAAGAATCAGTAAGTTCGCGCACAGTGATGCCTAGGTacct                                                                                                                                           >  1:499630/1‑146 (MQ=255)    gATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAAGGCCAAGAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATa                                                                                                                                          >  2:117096/1‑149 (MQ=255)      tGTACATCATCATGCTGCCCCCCATATCGTTGGTAAAGAAATGGGTGCCCAGAGAGCGATCCAGGGTCAACAACGCGACGGGAACCGTCAGAATTTGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACt                                                                                                                                        <  1:381830/149‑1 (MQ=255)        tACATCATCATGTTGCCACCCATATCGATGCTGTCAATATTTGTGCCCAGATAGCGCTCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGCAGGAAGCAATAATCAGTACGCTCGCGCACAGTAATGCCTCGGGAAATACTgg                                                                                                                                      >  1:359086/1‑149 (MQ=255)          catcatcatGTTGCCACCCATATCGTGGGTAAAGAAATGGGTGCCCAGATAGCGATCGAGGGTCAACAACGCGGCGGTAACCGTCAGAATTGGGAAGGAAGCAATAGTCAGTAGGTTAGCGCACGGTGATGCCTAGGTAGAGACTGGCa                                                                                                                                    >  1:516151/1‑149 (MQ=255)             catcatGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCt                                                                                                                                 >  1:723303/1‑149 (MQ=255)              atcatGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCtt                                                                                                                                >  2:603766/1‑149 (MQ=255)               tcatGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTg                                                                                                                               <  2:211477/149‑1 (MQ=255)                 atGTGGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGaa                                                                                                                             <  2:244096/149‑1 (MQ=255)                     tGCCACCCATTTTGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATg                                                                                                                         <  2:719622/149‑1 (MQ=255)                             gtatCGTTGGGAAAGAAATGGGTGCACAGATAGCGATTCAGGGTCAACAACGCGGCGGTAACCGTCAGAAGTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGcc                                                                                                                 <  2:50663/148‑1 (MQ=255)                                   ttGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTgc                                                                                                           >  2:271235/1‑149 (MQ=255)                                     ggTATAGAAATGGGTGCCCAGATAGGGATCCAGGGTCAACAACGCGACGGTAACAGTTAGAATTGGGAAGGAAGCAATCATCAGTACGTGCGCGCACAGTGATGCCTAGGTAAACACTGGCATCTTGAACATGGTCATGCCCGGTgcgc                                                                                                         <  2:93747/149‑1 (MQ=255)                                       tAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGGAGGAAGCAGTAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAGCATGGGCATGCCCGGTgcgcga                                                                                                       >  2:394206/1‑148 (MQ=255)                                       tAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTgcgcgc                                                                                                       >  2:211776/1‑149 (MQ=255)                                        aaaGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGAAGCCTAGGAAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCa                                                                                                      >  1:509179/1‑149 (MQ=255)                                         aaGAAATGGGTGCCCAGATAGGGATCCAGGGTCAAGAACGCGACGGTAACCGTCAGAATTGGGGAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTgcgcgccg                                                                                                     >  1:774623/1‑147 (MQ=255)                                         aaGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCAt                                                                                                     >  1:199772/1‑149 (MQ=255)                                             aaTGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTc                                                                                                 <  1:63842/149‑1 (MQ=255)                                                gggTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGa                                                                                              >  2:163644/1‑149 (MQ=255)                                                  gTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTGATGCCCGGTGCGCGCATCTTCaaaac                                                                                            >  2:244221/1‑148 (MQ=255)                                                       cAGATGGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTCAAGCCTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTaa                                                                                       <  1:321055/149‑1 (MQ=255)                                                                aTCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGt                                                                              >  1:611600/1‑149 (MQ=255)                                                                   cAGGGTCAACAACGCGACGGTAACCGTTAGAATTGGGAAGGAAGCAATAATCAGTACGGTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGa                                                                           <  2:199363/149‑1 (MQ=255)                                                                    aGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGAt                                                                          >  2:350913/1‑149 (MQ=255)                                                                      ggTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCGGTAGGGGAACGAAGAAGTTGATAc                                                                        >  1:579906/1‑149 (MQ=255)                                                                                gcgACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTAATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGcgt                                                              >  2:479265/1‑149 (MQ=255)                                                                                gcgACGGTAACCGTCAGAAGTGGGAAGGAAGCAATAATCAGTACGTTCGCGCATAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGCCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGcgt                                                              <  2:651161/149‑1 (MQ=255)                                                                                   aCGGGAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGcgtcgt                                                           <  2:655430/149‑1 (MQ=255)                                                                                    cGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTa                                                          <  1:163644/149‑1 (MQ=255)                                                                                     ggTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGTGTCGTAc                                                         <  2:723303/149‑1 (MQ=255)                                                                                      gTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGCTGCATCGTGGAAGACTGGCCCATTTTTCAACTACATGCCCGGTGCGCGCAGCTTCTTAATTGAAACTAAGAAATTGATACCGGTAAGCGTCGGAcc                                                        >  2:763235/1‑149 (MQ=255)                                                                                            gTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCCTCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATGCCGGTAAGCGTCGTACCTATccc                                                  >  1:54294/1‑149 (MQ=255)                                                                                                gAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGAt                                              <  1:272476/149‑1 (MQ=255)                                                                                                    tGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCt                                          <  1:603766/149‑1 (MQ=255)                                                                                                     gggAAGGAAGCAATAATCCGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTg                                         <  2:50472/149‑1 (MQ=255)                                                                                                      ggaaggaaGCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTgg                                        <  1:291823/149‑1 (MQ=255)                                                                                                       gaaggaagCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGga                                       >  1:107806/1‑149 (MQ=255)                                                                                                            aagCAATAATCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTc                                  <  1:211776/149‑1 (MQ=255)                                                                                                                aataatCAGTACGTTCGCGCACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATCGCCGGCGCCTCCata                              >  2:326088/1‑149 (MQ=255)                                                                                                                 ataatCAGTACGTTCGCGCACAGGGATGACTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCatat                             >  2:428971/1‑149 (MQ=255)                                                                                                                          aCGTTCGCGAGCAGGGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATc                    <  1:335811/149‑1 (MQ=255)                                                                                                                              tCGCGCACAGTGATGCCTAGGTAAATACCGGCATCTTGAACATGGTCATGCCCGGGGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGaca                <  2:384230/149‑1 (MQ=255)                                                                                                                                gcgcACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATAGCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACAcc              <  2:77011/149‑1 (MQ=255)                                                                                                                                  gcACAGTGATGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACGCCATATCCAGTAATCGACACCGa            >  1:635576/1‑149 (MQ=255)                                                                                                                                      aatGATGTCTAGGTAATTACTGGCATCTGGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTcc        <  2:7778/147‑1 (MQ=255)                                                                                                                                          aTGCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGa    <  2:199772/149‑1 (MQ=255)                                                                                                                                            gCCTAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACt  <  2:12184/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                  GTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACT  >  CP009273/445267‑445554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑