| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 367,764 | A→T | 19.6% | H65L (CAT→CTT) | mhpD → | 2‑keto‑4‑pentenoate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 367,764 | 0 | A | T | 19.6% | 39.8 / 2.2 | 20 | H65L (CAT→CTT) | mhpD | 2‑keto‑4‑pentenoate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (9/7); new base T (2/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTT > CP009273/367625‑367905 | cAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGt > 1:186999/1‑149 (MQ=255) gCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAGATGGGCAACATGACGTTGCGCAGGGGGGGCGGGTGGGAGGGCGTAAAGTGGGCCGGACACATCCGAAAGTGCAACAACAACTggg > 1:388704/1‑149 (MQ=255) aCCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAGCAACt > 2:110069/1‑144 (MQ=255) aCCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCg > 1:125030/1‑149 (MQ=255) cGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTgg > 2:339242/1‑144 (MQ=255) cGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGtt > 1:53797/1‑149 (MQ=255) gcttgATAACGCTGAAGGGGCCTACGCCATTCAGCACATAAATGTGGAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAACGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGAttt < 2:80349/140‑1 (MQ=255) gATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACTTCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTggg > 2:223292/1‑144 (MQ=255) gATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACTTCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTggg > 2:302368/1‑144 (MQ=255) gCTGAAGCGGCTTACGCCATTCAGCGCATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGc > 1:222395/1‑149 (MQ=255) cGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATg < 1:371460/149‑1 (MQ=255) aTAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAAt > 1:286439/1‑149 (MQ=255) tgtgCAACCTGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTc < 1:46724/149‑1 (MQ=255) tCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTCGCCCACTTGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCa < 1:210774/149‑1 (MQ=255) aGGGCGTAAAGTGGGCCTGACACTTCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCgg < 1:336539/149‑1 (MQ=255) aGGGCGTAAAGTGGGCCTGACACTTCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCgg < 1:251600/149‑1 (MQ=255) gggCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACCTGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGaa > 2:235457/1‑144 (MQ=255) cGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCg < 2:47840/144‑1 (MQ=255) aaGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCa < 1:346698/149‑1 (MQ=255) tGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCg < 2:327212/144‑1 (MQ=255) acaTCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGGGtt > 2:121624/1‑144 (MQ=255) | CAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCCATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTT > CP009273/367625‑367905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |