| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,048,586 | A→T | 100% | V18E (GTG→GAG) | glnG ← | fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,048,586 | 0 | A | T | 65.0% | 14.2 / 13.7 | 20 | V18E (GTG→GAG) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base T (10/3); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
| Rejected as polymorphism: Not supported by required number of reads on both strands. | |||||||||||
CAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGC > CP009273/4048444‑4048725 | cagcGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAAc < 1:38106/149‑1 (MQ=255) agcGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCATGCTCAAGCTcc > 2:289784/1‑144 (MQ=255) cGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAACCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTc > 2:83190/1‑144 (MQ=255) atatCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGAc < 1:274863/149‑1 (MQ=255) tGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATcc > 1:16654/1‑149 (MQ=255) aTCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCTAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTg > 2:15254/1‑144 (MQ=255) cGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTAAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACg > 1:147408/1‑149 (MQ=255) ggCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCACGGCGAGCCCACGCCCAACCACCCAACGGATGGAACGATCACCATCGACTACCCAGACTATCCCTCGCTACATa > 2:126917/1‑144 (MQ=255) tCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAACCGTCACCt > 2:243604/1‑144 (MQ=255) tCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCt > 2:314802/1‑144 (MQ=255) cAGCGCCTCCCGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCt < 1:146372/149‑1 (MQ=255) gCACTTCTGCGCCGTTCTCAAACGTGGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTAGCATAAACGTCACCTTTATTTCCTGATAGGCAGGt > 1:221812/1‑149 (MQ=255) tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGcc > 1:254412/1‑149 (MQ=255) tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTACCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCCGGTCAACCGAGAACTCGGTATGccc > 2:303706/1‑144 (MQ=255) aGGTTAATCCTGCCCCAGCGTGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAc < 1:145301/149‑1 (MQ=255) gTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAAACTATCCCTCGTTGCATAAACGCCCCCTTTATTTTCTAATGGGCAGGTAAACCGAGAACTCGGGATGCCCTGGcc > 2:37435/1‑144 (MQ=255) gTTAAACCTGCCCCAGCGAGCGCACGGTCAAGCTCCCAACGTTTGGCACGATCGGCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCCCCTTTATTTCCTGAGAGGCAGGCAAACCGAGAACCCCATATGCCCTGGcc > 2:96872/1‑144 (MQ=255) aaaCCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTa > 1:2993/1‑149 (MQ=255) tGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCACCTGGTa > 2:54171/1‑144 (MQ=255) aaGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTGAATTCAATTTTGCCTGAATGc > 2:130521/1‑144 (MQ=255) | CAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGC > CP009273/4048444‑4048725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |