| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,062,550 | G→A | 16.7% | intergenic (‑3/+18) | yccJ ← / ← wrbA | uncharacterized protein/NAD(P)H:quinone oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,062,550 | 0 | G | A | 16.7% | 48.0 / 4.2 | 24 | intergenic (‑3/+18) | yccJ/wrbA | uncharacterized protein/NAD(P)H:quinone oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (9/11); new base A (2/2); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
TCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTA > CP009273/1062417‑1062687 | tcCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGaa > 1:129455/1‑149 (MQ=255) ccctctCCGCCAGTTGTTCGTGTGTCCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTCCGTTTTTGTTGGGTGCATGCGTATCCTCCTGTTGAAGa < 2:345198/139‑1 (MQ=255) gCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTGGCTGCTTGAGTTGGCATGCGTATCCTCCTGTTGAGGATTTGGCGTTAAGt > 1:250330/1‑149 (MQ=255) aTTTTCTCGTCCTACCCGGCGCCTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCCTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTa < 1:162222/149‑1 (MQ=255) ttttttCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTaa < 1:143426/149‑1 (MQ=255) ttttCGTCGAACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTACTGCGCAGAGATGCCCATTCAACGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACt < 1:396585/149‑1 (MQ=255) tcgtACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTg > 2:245243/1‑144 (MQ=255) ccGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGTAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCg > 1:256261/1‑149 (MQ=255) ttCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGTGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCATATCCTCCTGTTGAAGATTGGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTc > 1:1202/1‑149 (MQ=255) ttCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGTGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCATATCCTCCTGTTGAAGATTAGCCGTTACGTTTGACTTCCAGACCTGCGACATATTc > 1:107827/1‑149 (MQ=255) ttCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCGTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACa > 2:119930/1‑144 (MQ=255) aaaTGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTc < 2:35812/144‑1 (MQ=255) gCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGCGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCCGACCTGCGACATATTCCCCTTGATac > 1:43070/1‑148 (MQ=255) ccTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAAc > 1:110902/1‑149 (MQ=255) cTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGATTCGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGa < 2:20568/144‑1 (MQ=255) tCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGa < 1:164243/149‑1 (MQ=255) cGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAGCGAGCAATAgcc > 2:39223/1‑142 (MQ=255) tGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCg < 1:282340/149‑1 (MQ=255) tGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCg < 1:220194/149‑1 (MQ=255) aTTCACCGACGTGATGAGCTTTCGCATCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAAc > 1:238174/1‑149 (MQ=255) gACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCg < 2:69374/144‑1 (MQ=255) ttcttGGGTTGGCATGCATATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGAtggtg < 2:982/144‑1 (MQ=255) gtcttGAGTTGGCATGCATATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGAtggtg < 2:96175/143‑1 (MQ=255) gCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTa < 2:114831/144‑1 (MQ=255) | TCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTA > CP009273/1062417‑1062687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |