| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,419,933 | G→T | 67.2% | A112D (GCC→GAC) | hisJ ← | histidine/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,419,933 | 0 | G | T | 67.2% | 18.0 / 13.7 | 24 | A112D (GCC→GAC) | hisJ | histidine/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (3/5); new base T (8/8); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
TTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAA > CP009273/2419804‑2420062 | tttGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTAGACTGTCGGCTGAATGTCAGGATTTTTCGCCACCACCAAACGAGAATCGGCATCGTACAGTTTGGCGGt > 1:258/1‑149 (MQ=255) gTGCCCAATGTTCATTACCTAACGTCTCCTGGATGGTGCCTTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAg < 1:190082/149‑1 (MQ=255) aTGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGGTTGTCGGTGAAGGCTAttt > 1:4744/1‑149 (MQ=255) aCCGGACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGAc < 1:132934/149‑1 (MQ=255) tggtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGACTTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGGGAAGGCTCTTGCTTGCTGACGTTTTTCCGTaa > 2:63459/1‑144 (MQ=255) gtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATg < 2:314882/144‑1 (MQ=255) gtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATg < 2:165354/144‑1 (MQ=255) cTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAg > 2:256503/1‑144 (MQ=255) tGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTGATGGAAAGCGATGa > 1:101133/1‑149 (MQ=255) ccaCCCGTTTGCCTTTCAGCGACTCGACTGCCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACatga < 2:305437/142‑1 (MQ=255) cTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTCCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCg < 1:313446/149‑1 (MQ=255) tttGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGAAATGATGGCGTCAAtct > 1:148409/1‑149 (MQ=255) ccTTTCAGCGCCTCGACTGTCGGCTGAATGTCAGAATTTGTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAAtc < 2:319631/144‑1 (MQ=255) tttCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCGGCGTACAGTTTGTCGGGGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGTGGGCAATCTCCTTCg > 1:66761/1‑149 (MQ=255) gactcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTaa > 1:108519/1‑149 (MQ=255) ctcgactGTCGGCTTAATGTCAGGATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAgg < 1:69999/149‑1 (MQ=255) ctcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCGCCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCttt < 2:56595/144‑1 (MQ=255) actGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAg < 2:102865/144‑1 (MQ=255) ctGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACggg > 1:272629/1‑149 (MQ=255) gCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAAc < 1:334586/149‑1 (MQ=255) aaTGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTaa > 2:111332/1‑144 (MQ=255) ttCACCACCCCCAAACGAGAACCCTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCCGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGa < 2:304101/144‑1 (MQ=255) ccaccaAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATGAACGCATCCAGCGGATTTTCGa < 2:4043/144‑1 (MQ=255) ccaAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGCTTTTCGACaa > 2:119717/1‑144 (MQ=255) | TTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAA > CP009273/2419804‑2420062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |