Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,362,541 (G)5→4 intergenic (+53/‑85) groL → / → yjeI Cpn60 chaperonin GroEL, large subunit of GroESL/DUF4156 family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,362,5370G.98.8% 366.9 / ‑0.0 84intergenic (+49/‑89)groL/yjeICpn60 chaperonin GroEL, large subunit of GroESL/DUF4156 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base . (36/45);  minor base A (0/1);  total (36/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCCGCGCTACTGATGGCGGGTT  >  CP009273/4362389‑4362680
                                                                                                                                                     |                                                                                                                                               
caccAAACGCATCGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATCTAATTGCCCTGCACCTCGCAGTAATATACAAACCCCCGGGCAGAAATGTCTg                                                                                                                                                 <  1:711758/149‑1 (MQ=255)
         cATGGTTACCGACCTGCCGAAAACCGATGAGCAGTTGGTAGCCGCGGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACATCGCCGATATCTAATAATCCACGGGCAGCAATGTCTggggcaaac                                                                                                                                         <  2:875602/148‑6 (MQ=255)
         cATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTc                                                                                                                                       <  1:1355737/149‑1 (MQ=255)
              ttACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGAACAGTTGTCTGTGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTg                                                                                                                                  <  1:997926/149‑1 (MQ=255)
               tatcgtactgctgAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTTGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTg                                                                                                                                  <  2:292316/141‑1 (MQ=255)
               tACCGCCCTGCCGAAAACCGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTg                                                                                                                                  <  2:658745/148‑1 (MQ=255)
                  cGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAACGTCTGGGGTGTTTCTTTTGGTCa                                                                                                                              >  1:505149/1‑149 (MQ=255)
                       tGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGGCTGGGGTATTTCGTTTGGTCATCttt                                                                                                                         >  1:1026379/1‑149 (MQ=255)
                        gCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGGATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCttt                                                                                                                         >  2:270514/1‑148 (MQ=255)
                        gCCGAAAAACGATGCAGCTGACTTAGGCGCCGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCACCATGTGTGTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCttt                                                                                                                         <  2:492255/148‑1 (MQ=255)
                          cGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCt                                                                                                                       >  2:298559/1‑148 (MQ=255)
                           gAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTa                                                                                                                      >  2:949850/1‑148 (MQ=255)
                           gAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAg                                                                                                                     <  1:689659/149‑1 (MQ=255)
                              aaaCGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAAGGACTGGGGTTTTGCTTTTGGTCATCTTTCTAGtat                                                                                                                  >  1:32618/1‑149 (MQ=255)
                               aaCGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGtat                                                                                                                  >  2:1241662/1‑148 (MQ=255)
                               aaCGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGtat                                                                                                                  >  2:821784/1‑148 (MQ=255)
                               aaCGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTGTTCTTTTGGTCATCTTTCTAGtcta                                                                                                                 >  1:368898/1‑149 (MQ=255)
                                aCGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGCTTTCCCTTTGGTCATCTTTCTAGTGTaa                                                                                                                >  1:589991/1‑149 (MQ=255)
                                  gATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGTGTTTTTCTTTTGGTCATCTTTCTAGTATAAGa                                                                                                              <  1:73177/149‑1 (MQ=255)
                                   aTGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGGATAAGa                                                                                                              >  2:715557/1‑148 (MQ=255)
                                   aTGCAGCTGACGTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGa                                                                                                              <  2:389891/148‑1 (MQ=255)
                                             aCTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGTTCATCTTTCTAGTATAAGATTCAGACACg                                                                                                    <  1:833734/149‑1 (MQ=255)
                                             aCTTAGGCGCTGCCGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACACCCGGGATGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACg                                                                                                    >  1:935966/1‑149 (MQ=255)
                                               ttAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAGTGGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGAGTCAGACACgg                                                                                                   >  2:820074/1‑148 (MQ=255)
                                               ttAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTCGTCTAAGATTCAGACACgg                                                                                                   >  2:92114/1‑148 (MQ=255)
                                                tAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGga                                                                                                  <  2:849017/148‑1 (MQ=255)
                                                   gcgcTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGCTTCAGACACGgacgac                                                                                              >  1:578011/1‑149 (MQ=255)
                                                   gcgcTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGCTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGgacgc                                                                                               >  2:1081383/1‑147 (MQ=255)
                                                    cgcTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGacgacg                                                                                             >  1:611025/1‑149 (MQ=255)
                                                    cgcTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGAAGTAATTACCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGAGACGGacgacg                                                                                             >  1:610806/1‑149 (MQ=255)
                                                      ctgctgGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGAcgc                                                                                            <  2:874542/148‑1 (MQ=255)
                                                       tgctgGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAAGGTCTGGGGTATATATTTTGGTCATCTTTCTAGTATAAACGTCAGACACGGACGAcgcg                                                                                           >  2:676450/1‑148 (MQ=255)
                                                        gctgGCGGTATGGGCGGCACGGGTGGGATGGCCGGCATGATGTAATTGCCCTGCACCTCGCAGAAAGAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGTTTCAGACACGGACGACGCGa                                                                                          <  2:173109/148‑1 (MQ=255)
                                                         ctgGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGAGTCAGACACGGACGACGCGAGt                                                                                        <  1:970427/149‑1 (MQ=255)
                                                          ctgCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGGCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTg                                                                                       <  1:1175011/147‑1 (MQ=255)
                                                           ggCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTgg                                                                                      <  1:705378/149‑1 (MQ=255)
                                                           ggCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTg                                                                                       <  2:647274/148‑1 (MQ=255)
                                                             cGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGc                                                                                     <  2:520078/148‑1 (MQ=255)
                                                                tATGGGCGGCATGGGTGGTATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTc                                                                                  <  2:1080304/148‑1 (MQ=255)
                                                                tATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTc                                                                                  <  2:454071/148‑1 (MQ=255)
                                                                tATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTc                                                                                  <  2:927526/148‑1 (MQ=255)
                                                                 aTGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCa                                                                                <  1:1152374/149‑1 (MQ=255)
                                                                  tGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAg                                                                               <  1:1162974/149‑1 (MQ=255)
                                                                    ggcggcATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACCCGAGTGGCGTCCAGc                                                                              >  2:1042550/1‑148 (MQ=255)
                                                                      cggcATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCGGCTc                                                                            >  2:37941/1‑148 (MQ=255)
                                                                      cggcATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGGGGCGTCCAGccc                                                                            >  2:996459/1‑146 (MQ=255)
                                                                      cggcATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGATTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGGGTGGCGTCCCGcaca                                                                           >  1:1231281/1‑149 (MQ=255)
                                                                           tGGGTGGCACGGGCGGCGTGATGTAATTGCCCTGCCCCACCCAGAAATAAACACACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCAGCTTTCTAATAGGAGATTCATACACGGACGACGCGAGTGGCGTCCAGCTCATTGa                                                                       <  2:453184/148‑1 (MQ=255)
                                                                             ggTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGAtt                                                                     <  2:1053170/148‑1 (MQ=255)
                                                                              gTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTAt                                                                   <  1:1189535/149‑1 (MQ=255)
                                                                                 gCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATgg                                                                 >  2:6572/1‑148 (MQ=255)
                                                                                 gCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCAGTGATTATgg                                                                 >  2:621305/1‑148 (MQ=255)
                                                                                  gaTGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATggg                                                                <  2:555050/147‑1 (MQ=255)
                                                                                  cATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGATTTTCTTTTGGTCATCTTTCTAGTATAAGATTCCGACACGGACGACGCGAGGGGCGTCCAGGTCATTGATGATggg                                                                >  2:318794/1‑148 (MQ=255)
                                                                                  cATGGGCGGCATGATGTAAGTGCCCTGCCCCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATggg                                                                <  2:994208/148‑1 (MQ=255)
                                                                                     gggCGGCATGATGTAATTGCCCTGCACCTAGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAAt                                                            >  1:834143/1‑149 (MQ=255)
                                                                                          gcATTATGGAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACAt                                                       <  1:1358678/149‑1 (MQ=255)
                                                                                          gcATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACa                                                        <  2:1167978/148‑1 (MQ=255)
                                                                                          gcATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACAt                                                       <  1:1162136/149‑1 (MQ=255)
                                                                                           cATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACAt                                                       >  2:147476/1‑148 (MQ=255)
                                                                                            atgatgTAATTGCCCTGCAGCTCGCTAAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGc                                                     <  1:1262880/149‑1 (MQ=255)
                                                                                              gatgTAGTTGCCCTGGACCTCGCGGAAATAACCAAACCCCCGGGCAGAAAGGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCAc                                                   <  1:98280/149‑1 (MQ=255)
                                                                                              gatgTAAATGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCGGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCAc                                                   <  1:296450/149‑1 (MQ=255)
                                                                                               atgTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGGTGCGTCCACCCCATTGATTATGGGGAATAACATGCACg                                                  >  1:458572/1‑149 (MQ=255)
                                                                                               atgTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTGTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCAc                                                   <  2:482978/148‑1 (MQ=255)
                                                                                               atgTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGATTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCGGCTCGTTGATTATGGGGAATAACATGCAc                                                   >  2:89171/1‑148 (MQ=255)
                                                                                                tgTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGt                                                 >  1:895115/1‑149 (MQ=255)
                                                                                                     ttGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGACTATGGGGAATAACATGCACGTaaaa                                             >  2:549728/1‑148 (MQ=255)
                                                                                                      tGCCCTGCACCCCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCCGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATa                                           <  1:1318923/149‑1 (MQ=255)
                                                                                                         ccTGCTCCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACt                                         <  2:740572/148‑1 (MQ=255)
                                                                                                                 tCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGa                                <  1:1346027/149‑1 (MQ=255)
                                                                                                                     aGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGt                            <  1:175197/149‑1 (MQ=255)
                                                                                                                     aGAAAAAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGt                            <  1:42443/149‑1 (MQ=255)
                                                                                                                      gAAATTAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGCGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTc                           <  1:607321/149‑1 (MQ=255)
                                                                                                                        aaTAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCgg                         <  1:1203605/149‑1 (MQ=255)
                                                                                                                        aaTAAACAAACCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCg                          <  2:593594/148‑1 (MQ=255)
                                                                                                                            aacgaacCCCCGGGCAGAAAGGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGAATAAGATTCAGACACGGACGACGAGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGCTTGTCGgcgc                      >  2:148273/1‑148 (MQ=255)
                                                                                                                            aacaaacCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGc                      >  2:1103581/1‑148 (MQ=255)
                                                                                                                               aaacCCCCGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTCTGGGGAATAACATGCACGTAAAATACTTAGCGGGGATTGTCGGTGCcgcg                  >  1:210852/1‑149 (MQ=255)
                                                                                                                               aaacCCCCGAGCAGAAATGTCTGGGGTTTTTCTTTTTGTCATCTTTCTAGTATAAGATTCAGACACGCACGACGCGAGTTGCGTCCATCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCcgcg                  <  1:616079/149‑1 (MQ=255)
                                                                                                                                   ccgcGGGCAGAAATGGCAGGGGTTCTTCATTTGGTCATTTTTCTAGTATTAGATTCAGACACGGCTGACGCGAGTGTCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTTAAATACTTAGCAGGGATTGTCGGTGCCGCGCTa               <  2:133347/145‑1 (MQ=255)
                                                                                                                                   ccccGGGGGGAAATGGCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCCGCGCTa               <  2:1184647/148‑1 (MQ=255)
                                                                                                                                   ccccGGGCAGAAATGTCTGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCCGCGCTAc              <  1:358395/149‑1 (MQ=255)
                                                                                                                                     ccGGGCAGAAATGTCTGGGGTTTTTCCTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCCGCGCTACTg            >  1:333661/1‑149 (MQ=255)
                                                                                                                                            gAAGTGTCTGGGGTTTTTCTTTTGGTTATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATGGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCCGCGCTACTGATGGCgg     <  1:958014/149‑1 (MQ=255)
                                                                                                                                             gaaacggcgggggTTTCTCTTTTGGCCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCCGCGCTACTGATGGCg      <  2:1020171/140‑1 (MQ=255)
                                                                                                                                               ctGTCTGGGGTTTTTGTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCCGCGCTACTGATGGCGGGtt  <  1:1038786/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAAGATTCAGACACGGACGACGCGAGTGGCGTCCAGCTCATTGATTATGGGGAATAACATGCACGTAAAATACTTAGCAGGGATTGTCGGTGCCGCGCTACTGATGGCGGGTT  >  CP009273/4362389‑4362680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: