Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,370,460 (T)5→6 coding (320/639 nt) sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,370,4551.T96.3% 167.6 / NA 55D109N (GAC→AAC) sspAstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base T (22/30);  minor base G (1/1);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGG  >  CP009273/3370312‑3370597
                                                                                                                                                |                                                                                                                                               
cAGGCTGAACTCATCGCCCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTTAAGAGTTTGTTCATCAGCGTGTACCAGTCttttt                                                                                                                                           <  1:332694/149‑1 (MQ=255)
  ggCTGAACTCCTCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCtttttt                                                                                                                                          <  2:945567/148‑2 (MQ=255)
  ggCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCtttttt                                                                                                                                          <  2:872176/148‑2 (MQ=255)
  ggCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCtttttt                                                                                                                                          <  2:111584/148‑2 (MQ=255)
   gCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCttttttcg                                                                                                                                        <  1:186631/149‑4 (MQ=255)
    cTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATATGCTTCAGAAGCTGCACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCttttttcg                                                                                                                                        <  2:919464/148‑4 (MQ=255)
     tGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCttttttcgat                                                                                                                                      >  1:1087866/1‑144 (MQ=255)
     tGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCttttttcgag                                                                                                                                      >  1:49021/1‑144 (MQ=255)
       aaCTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTcgatgc                                                                                                                                     <  1:804673/149‑1 (MQ=255)
         cTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTcgatgcga                                                                                                                                   <  1:459675/149‑1 (MQ=255)
            aTCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTcgatgcgatg                                                                                                                                 >  2:1222782/1‑148 (MQ=255)
             gcccggagaaaGTCCGGCTTCGGATCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCgatgggatgc                                                                                                                                <  2:580721/139‑1 (MQ=255)
              cGCTCAGGAAGTACGGCTTCTGACGGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCAt                                                                                                                              <  1:1226761/149‑1 (MQ=255)
              cGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCAt                                                                                                                              <  1:41939/149‑1 (MQ=255)
               gCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATg                                                                                                                             >  1:1274222/1‑149 (MQ=255)
                 tCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGt                                                                                                                            >  2:169050/1‑148 (MQ=255)
                     gAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGa                                                                                                                       >  1:1176366/1‑149 (MQ=255)
                     gAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGa                                                                                                                       <  1:553267/149‑1 (MQ=255)
                         tACGGCTTCTGACCGAAGACCGGCGCAATCTCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGc                                                                                                                   >  1:711440/1‑149 (MQ=255)
                              cTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTc                                                                                                               <  2:1038071/148‑1 (MQ=255)
                               ttCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGGGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCa                                                                                                              <  2:644393/148‑1 (MQ=255)
                                   gACCGAAGACCGGCGCAATCGCCAGCCGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTGATCAGCTTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACcgc                                                                                                          <  2:808792/148‑1 (MQ=255)
                                    aCCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGa                                                                                                        <  1:676303/149‑1 (MQ=255)
                                    aCCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGa                                                                                                        <  1:744485/149‑1 (MQ=255)
                                      cGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTCCCAGTCTTGTTTCGATGCGATGCCTGTACAGCCGGCTTTCACCGCGAGc                                                                                                      >  1:523000/1‑149 (MQ=255)
                                      cGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGGTGCATGTACAGACGGCTTTCACCGCGAg                                                                                                       >  2:821242/1‑148 (MQ=255)
                                      cGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGc                                                                                                      >  1:898162/1‑149 (MQ=255)
                                      cGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGCTCCTCCGCGTGTACCCGTCTTTTTTCGGTGCGAGGCATGTACAGACGGCTTGCAGcggcgg                                                                                                       >  2:615308/1‑144 (MQ=255)
                                       gAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCt                                                                                                     >  1:272901/1‑149 (MQ=255)
                                         cgcccGGCGCGATCGCCAGCAGGTGTTCGCGGAGTTGCTTGCGTGCGGCATCTGCTTCCGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGCTGCGATGCATGTACAGACGGCTTTCACCGCGAGCTa                                                                                                    <  2:1064033/145‑1 (MQ=255)
                                           aCCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCg                                                                                                 <  1:556428/149‑1 (MQ=255)
                                            ccGGCGCAATCGCCAGCAGTTTTTCGCGCAGTTGCTTACGTGCGGCATCGGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCgg                                                                                                <  1:626639/149‑1 (MQ=255)
                                               gcgcAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGCGTACCAGTCTTTTTTCGATGCGATGCCTGTACAGACAGCTTTCACCGCGAGcaccagggg                                                                                              >  2:1226481/1‑140 (MQ=255)
                                                    aTCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAg                                                                                        <  1:945005/149‑1 (MQ=255)
                                                     tCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGGGCTACCGGGTAAACAgg                                                                                       >  1:311943/1‑149 (MQ=255)
                                                     tCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAgg                                                                                       >  1:311903/1‑149 (MQ=255)
                                                     tCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGGAAACAgg                                                                                       >  1:558819/1‑149 (MQ=255)
                                                              gTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTgg                                                                              <  1:1326648/149‑1 (MQ=255)
                                                               ttcttcGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTgg                                                                              <  2:1035769/148‑1 (MQ=255)
                                                                 cttcGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGACATCAGTGGCgg                                                                           >  1:159417/1‑149 (MQ=255)
                                                                      cgcAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTTACCGCGTGCTACCGGGTAAACAGGCATCAGTGGCGGATGCg                                                                      <  1:1212868/149‑1 (MQ=255)
                                                                         aGTTGCTTACGTGCGGGATCTGCTTCAGAAGCTGAACCGTTGATGGTGGTGTTCATCAGCGTGTACCAGACGTTTTTCGATGCGATGCATGTACAAACGGCTTTCACCGCGGGCTACGCGGGAAAGAGGCCTCGATGGCgggtctggg                                                                    >  2:520838/1‑141 (MQ=255)
                                                                               ttACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCAACAGTGGCGGAGGCGGGAAACga                                                              >  2:206391/1‑147 (MQ=255)
                                                                                 aCGTTCGGCATCTGCTTCGGAAGCGGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTc                                                            <  2:446433/148‑1 (MQ=255)
                                                                                 aCGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCa                                                           >  1:746338/1‑149 (MQ=255)
                                                                                      cGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCAGGAGTGGCGGATGCGGGGAACGCACATCCa                                                       >  2:437726/1‑148 (MQ=255)
                                                                                       ggCATCTGCGTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAg                                                      <  2:868655/148‑1 (MQ=255)
                                                                                            cTGCGTCAGAAGCTGAACCGTTGATGATGGTGTTCAGCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATAtt                                                 <  2:644778/148‑1 (MQ=255)
                                                                                             tGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAACCGCTCATCCAGATATTcc                                               >  1:682168/1‑149 (MQ=255)
                                                                                              gCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCa                                              >  1:1345847/1‑149 (MQ=255)
                                                                                                        cTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCg                                    >  1:1254642/1‑149 (MQ=255)
                                                                                                         tGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCg                                    <  2:759374/148‑1 (MQ=255)
                                                                                                               gTTGAGGATGGTGTGCATCGGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTc                              <  2:697896/148‑1 (MQ=255)
                                                                                                                    tgatgGTGTTCATCAGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCcaca                         <  2:179427/148‑1 (MQ=255)
                                                                                                                    tgatgGTGTTCATCAGCGTGTACCAGGCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCcaca                         <  2:565564/148‑1 (MQ=255)
                                                                                                                        ggTGTTCCTCGGTGTGTACCAGTCGTTTTTCGATGCGATGCATGTACAGCCGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGt                     <  2:71927/148‑1 (MQ=255)
                                                                                                                         gtgtTCATCAGCGTGTACCGGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTc                    <  2:803168/148‑1 (MQ=255)
                                                                                                                              catcaGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCa              <  1:394072/149‑1 (MQ=255)
                                                                                                                               atcaGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCAc             >  1:172571/1‑149 (MQ=255)
                                                                                                                                tcaGCGTGTACCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGGAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACg            >  1:169342/1‑149 (MQ=255)
                                                                                                                                         aCCAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccacca    <  2:1014695/148‑1 (MQ=255)
                                                                                                                                          ccAGTCTTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCAACGGGGGCAGCTCACGTTCCACCAg   <  2:277142/148‑1 (MQ=255)
                                                                                                                                           cAGTCTTTTTTCGATGCGAGGCATGTACAGACGGCTTTCACCGCGAGCGACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAgg  <  2:814348/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGG  >  CP009273/3370312‑3370597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: