Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,353,426 A→C V69G (GTT→GGT)  rhmT ← putative L‑rhamnonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,353,4260AC98.6% 230.1 / NA 69V69G (GTT→GGT) rhmTputative L‑rhamnonate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (29/39);  minor base G (1/0);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACATACAGCGCCAACAT  >  CP009273/2353282‑2353571
                                                                                                                                                |                                                                                                                                                  
gcagcaCGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCAGGCCATGGCTGCCGAAAGAAATCCACACAGCAGAGTTGTCGTACCAATCCAGGCTCTGGCCCCACGGTTGCGCATCAAAAGATTCGCCGGAccac                                                                                                                                                 >  2:678454/1‑148 (MQ=255)
gcagcaCGAAGCAGAGTGCGAACTATCAGAAAGTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGaccacc                                                                                                                                                <  1:1123052/149‑1 (MQ=255)
    caCGCAGCGGAGTGCTAACTAGCAGAAAGTGCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGTAGTGTTGTCGTACCAATCCAGGTGCTGGCCCCCATTTTGCGCATCAAAAGATTCGCCGGACCACCCAg                                                                                                                                             <  2:622466/148‑1 (MQ=255)
     aCGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACGGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCCCCCAGa                                                                                                                                            >  2:53257/1‑148 (MQ=255)
              agTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCAGGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCAtata                                                                                                                                 <  1:127345/149‑1 (MQ=255)
               gTGCTAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAAGCCCCACCGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCAtata                                                                                                                                 <  2:1137942/148‑1 (MQ=255)
                tGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATAcc                                                                                                                               >  1:120772/1‑149 (MQ=255)
                    aaCTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACcaca                                                                                                                            <  2:712970/148‑1 (MQ=255)
                     aCTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTACCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACaaa                                                                                                                          <  1:398718/149‑1 (MQ=255)
                       tATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGa                                                                                                                        >  1:1190175/1‑149 (MQ=255)
                       tATCAGAAATTTCGCTTCAGTATCAGCCCATGCCCTGGCTGCCGAAAGAAATCCCCACAGCAGTGTGGTCGTACCAATCCAGCTTCTTGCCCCCAGTCTGCGCATCAAAACATTCGCCGCGCCACCCAGAAACGCATATACCACAAAg                                                                                                                         >  2:51485/1‑148 (MQ=255)
                        accagatccttCGCTTCAGTATCAACCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGaa                                                                                                                       <  1:923429/140‑1 (MQ=255)
                         tCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCCGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGaaa                                                                                                                      >  1:344378/1‑149 (MQ=255)
                           ataaaTTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAAt                                                                                                                    <  1:1235632/147‑1 (MQ=255)
                           aGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCGGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCGAATACCACAAAGAAAAt                                                                                                                    >  1:281044/1‑149 (MQ=255)
                           aGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGACCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGACTATACCACAACGaaaa                                                                                                                     >  2:101864/1‑148 (MQ=255)
                            gAAATTCCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGACATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAAt                                                                                                                    <  2:740397/148‑1 (MQ=255)
                               aTTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTGGTAGCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATCACACAAAGACAAGGcc                                                                                                                 >  2:442097/1‑148 (MQ=255)
                                tttCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGGTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGGAACGCATATACCAAAAAGAAAAAGccc                                                                                                                >  2:979020/1‑147 (MQ=255)
                                      ttCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCa                                                                                                         >  1:189711/1‑149 (MQ=255)
                                       tCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAg                                                                                                        <  1:202477/149‑1 (MQ=255)
                                           tATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCa                                                                                                    >  1:946533/1‑149 (MQ=255)
                                           tATCGGCCCATGCCATGGCTGCCGAAAGAAACCCCCACGGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACCCATATACCACAAAGAAAAGGCCTGCTCCCAgcgc                                                                                                     <  2:688758/148‑1 (MQ=255)
                                                gCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTCTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGACGGACCACCCAGAAACGCATATACCACAAAGAACATGCCCGCTCCGAGCGCATAAg                                                                                                >  2:375088/1‑148 (MQ=255)
                                                  ccatgccatgGCTGCCGAGAGAAATCCTCACATCAGTGTTGTCGTACCATTCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCt                                                                                              <  2:812588/148‑1 (MQ=255)
                                                  ccatgccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCtt                                                                                             <  1:19623/149‑1 (MQ=255)
                                                    aggcaatgGCTGCCGGAAGAAACCCCCACAGCATTGTTGTCGTACCCATCCAGGTCCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTc                                                                                            <  2:55060/143‑1 (MQ=255)
                                                     tgccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCAt                                                                                          <  1:451051/149‑1 (MQ=255)
                                                        catgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTa                                                                                        <  2:1011990/148‑1 (MQ=255)
                                                         atgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACt                                                                                      >  1:851937/1‑149 (MQ=255)
                                                               gCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAAAGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAAccc                                                                                <  1:1282601/149‑1 (MQ=255)
                                                                   aaagaaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTa                                                                            <  1:59477/149‑1 (MQ=255)
                                                                   aaagaaaTCCCCACAGCACTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGt                                                                             <  2:2916/148‑1 (MQ=255)
                                                                    gagaaaGCCCCACAGCAGGGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGAGCATCAAAAGATGCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTAt                                                                           <  1:222834/148‑1 (MQ=255)
                                                                    aagaaaTCCCCACAGCAGTGGTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTa                                                                            <  2:703188/148‑1 (MQ=255)
                                                                    aagaaaCCGCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTa                                                                            <  2:1156534/148‑1 (MQ=255)
                                                                       aaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCaa                                                                        <  1:1195340/149‑1 (MQ=255)
                                                                        aactcccACAGCCGTGTTGTCGTACCAATCCAGGTCCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCaa                                                                        <  2:522376/144‑1 (MQ=255)
                                                                         aTCCCCACCGCAGTCTTGTCGTACCCATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAATTCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATc                                                                      <  1:677320/149‑1 (MQ=255)
                                                                          tCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCt                                                                     >  1:115717/1‑149 (MQ=255)
                                                                             ccACCGCATTCTTGTCGTACCAATCCAGGTGCTCGCCCCCAGTTTGCGAATAAAAAGATTCGCCTTACCACCCATAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTgg                                                                   <  2:136120/148‑1 (MQ=255)
                                                                              cacaGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTa                                                                 <  1:1307624/149‑1 (MQ=255)
                                                                              cacaGCAGTGTTGTCGTACCAATCCAGGTTCTGGCACCCAGTTTGCGCATCAAAAGATTCGCCGGAGCACCCAGAAAAGCATATACCACAAAGACAATGCCTGCTCCCAGCGCATACGCTTCATTACCCAACACGGTATCACTCAGGt                                                                  >  2:387494/1‑148 (MQ=255)
                                                                               acaGCAGTGTTGTCGTACCAACCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTa                                                                 <  2:678500/148‑1 (MQ=255)
                                                                                   cagTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGCCCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCACCCCGCAATCACTCTGGTAGGACt                                                            >  1:178551/1‑149 (MQ=255)
                                                                                       gttgtCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGttt                                                        <  2:253191/148‑1 (MQ=255)
                                                                                          gtCGTACCAATCCAGGTGCTGGCCCCAAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGc                                                     <  1:203877/149‑1 (MQ=255)
                                                                                              tACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACACATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCTGGTATCAATCTGGTAGGTCTGTTTGGCaaaa                                                 >  1:919403/1‑149 (MQ=255)
                                                                                               aCCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTAAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAAc                                                >  1:862576/1‑149 (MQ=255)
                                                                                                 cAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCg                                              >  1:1170157/1‑149 (MQ=255)
                                                                                                  aaTCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGAATCAATCTGGTAGGTCTGTTTGGCAAAACCGa                                             >  1:254932/1‑149 (MQ=255)
                                                                                                    tCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCAGATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGata                                           >  1:1078112/1‑149 (MQ=255)
                                                                                                    tCCAGGTTCTGGCCCCCAGTCTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGat                                            <  2:256830/148‑1 (MQ=255)
                                                                                                      cAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATAtt                                         <  1:430529/149‑1 (MQ=255)
                                                                                                      cAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATAtt                                         <  1:158387/149‑1 (MQ=255)
                                                                                                       aGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTc                                        >  1:1352177/1‑149 (MQ=255)
                                                                                                       aGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTc                                        >  1:1144866/1‑149 (MQ=255)
                                                                                                           tCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGaa                                     <  2:1092811/148‑1 (MQ=255)
                                                                                                                 ccccAGTTTGCGCATCAAAAGATTCGCCGGACCACCCAGAAACGCCTGATACCGATATGGAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATCTTCGAACGGTCga                              <  1:722859/149‑1 (MQ=255)
                                                                                                                          gcgcATCAAAAGACTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATTTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCa                     >  1:934598/1‑149 (MQ=255)
                                                                                                                           cgcATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGAGATTCGAACGGTCGCGAAACGCCAg                    >  1:980867/1‑149 (MQ=255)
                                                                                                                           cgcATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCAGCTCCCAGCGCATAAGCTGCAATACTCAACCCGGAATGAATGAGGTAGGTCTGTTTGGCAAAACCGAAATTCGAACGATAGAGAAAcccaa                     >  2:1213548/1‑144 (MQ=255)
                                                                                                                            gcATCAAAAGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGTATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAg                    <  2:529650/148‑1 (MQ=255)
                                                                                                                                 aaaaGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTCCTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACATa              <  1:568924/149‑1 (MQ=255)
                                                                                                                                  aaaGATTCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGAATCACTCTGGTAGGTGTGTTTGGCACAGACGATATGCGAACGGACGAGGAACGCAAGcacaga              >  2:317816/1‑146 (MQ=255)
                                                                                                                                        tCGCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACATACAGCGcc       >  1:570273/1‑149 (MQ=255)
                                                                                                                                          gCCGGACCGCCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACATACAGCGCCa      <  2:105366/148‑1 (MQ=255)
                                                                                                                                          gCCGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTGATTAGTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACGTATATTCGAACGGTCGAGAAACGCCAGCACATACAtcgcgca     >  1:798106/1‑146 (MQ=255)
                                                                                                                                           ccGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACATACAGCGCCaa     <  2:1073849/148‑1 (MQ=255)
                                                                                                                                           ccGGACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTCCATTACTCAACCCGGTCACAACCCGGAAGGTCTCGTTGGCAAAACCGAAATTCGACCGGTCCAAAAACGCCAGCACATACAGCGCCaa     >  2:1089452/1‑148 (MQ=255)
                                                                                                                                             ggACCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCTGCGAGCCGTCTTCATTACCCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACATACAGCGCCAACAt  <  1:1275805/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCACATACAGCGCCAACAT  >  CP009273/2353282‑2353571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: