Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,219,839	C→G	R83G (CGT→GGT)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,219,839	0	C	G	98.6%	221.1 / NA	70	R83G (CGT→GGT)	ygjI	putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (31/38);  minor base T (0/1);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAT  >  CP009273/3219694‑3219981                                                                                                                                                  |                                                                                                                                               gCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGGGGAGGtt                                                                                                                                             >  1:22786/1‑149 (MQ=255)   ttGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATAATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTGAAAAGTTCGCTGGGCGGAGGTTgg                                                                                                                                           >  1:841509/1‑149 (MQ=255)      gACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAAGTGAGAAGCCGGTGTCTACGCGAGGGTCAAAAGTTCGCTGGGCGGAGGTTGGGc                                                                                                                                         >  2:681842/1‑148 (MQ=255)       aCTGTCCTCGGCACCGATGTTTTTCCTCGCTACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCAt                                                                                                                                       <  1:147391/149‑1 (MQ=255)         tGGCCTCGGCACCGATGTTTTTCCTCGCGTCGATTTTTTATTTTGTTCCCTTCTGTCTGATCATCGCAGGATTTGTTTCGTTTAATGAAAGCTCAGAAGCGGGTGTCTACGGGTGGGTAAAAAGTTTGGTGGGCGGGGGGTGGGCGtt                                                                                                                                      >  2:435957/1‑148 (MQ=255)           gCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATttat                                                                                                                                   <  1:683588/149‑1 (MQ=255)               cGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTA‑TCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTAAGAAGCCGGTGTCTACGCGTGGGTAAGAAGTTCGCAGGGCGGAGGTCCGACATttactcac                                                                                                                               >  2:407824/1‑143 (MQ=255)                   aCCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGACTTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGTTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGcc                                                                                                                            >  2:77114/1‑148 (MQ=255)                      gATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTGTCGGTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGGGGTTGGGCATTTATTACTGCCtat                                                                                                                         >  2:1055967/1‑148 (MQ=255)                       aTGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATAc                                                                                                                       <  1:1018897/149‑1 (MQ=255)                           ttttCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTTTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGACGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTAc                                                                                                                   <  1:595485/149‑1 (MQ=255)                                cgcgcgACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGt                                                                                                               <  2:877455/146‑1 (MQ=255)                                  cgcgACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTc                                                                                                             <  2:898462/148‑1 (MQ=255)                                     gacgaTTTTTTATTTTATTCCCTTCTGCCTGATCATCGCTGTCTTCGTGTTGTCGAGGAAAACCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGTCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTaa                                                                                                         <  1:262386/149‑1 (MQ=255)                                       cggttttttATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTaaa                                                                                                        <  2:107664/145‑1 (MQ=255)                                        gaTTTTTTATTTTATTCCCTGCTGTCTGATCATCGCAGAATTTGTTTCGTTATATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAGAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATCCCTACTGGTTCGTAAAc                                                                                                       <  2:924256/148‑1 (MQ=255)                                        gaTTTTTCATTTTATTCCCTTCGGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCCTGCGTAATAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAAc                                                                                                       <  2:1121997/148‑1 (MQ=255)                                          ttgtttagtttatTGCCTTCTTTATGAGCACCGCAGAATTTTTTTCGTTAAATAAAAACTCAGAGGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGTAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCt                                                                                                     <  2:372915/145‑1 (MQ=255)                                             tttattttatTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGGAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCAGTTATTACTGCCTATACCTACTGGTTCCTAGACCttgtc                                                                                                 >  1:223717/1‑145 (MQ=255)                                             tttattttatTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTc                                                                                                 <  1:761844/149‑1 (MQ=255)                                             tttattttatTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACATACTGGTTCGTAAACCTGtac                                                                                                 >  1:1031015/1‑147 (MQ=255)                                             tttattttatCCCCATCCGTCTGATCTTCGCAGAATTTTGTTCGTTAAATAAAAACTCAGACGCCGGGGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGCAGGGTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGtt                                                                                                  <  2:301895/148‑1 (MQ=255)                                               tattttattCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCtt                                                                                               >  1:127866/1‑149 (MQ=255)                                                 ttttattCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACACTAAATCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCtttt                                                                                             >  1:728749/1‑149 (MQ=255)                                                   ttattCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTc                                                                                           <  1:189109/149‑1 (MQ=255)                                                   ttactccactctgtctgATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCttttt                                                                                            <  2:60014/139‑1 (MQ=255)                                                    tattCTCTTCTGTCTGGTCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTc                                                                                           <  2:805009/148‑1 (MQ=255)                                                     attCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCa                                                                                          <  2:110814/148‑1 (MQ=255)                                                         ccTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTc                                                                                      >  2:18197/1‑148 (MQ=255)                                                          cTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCa                                                                                     <  2:93662/148‑1 (MQ=255)                                                           ttCTGTCTGATCATCGCCGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACt                                                                                   <  1:1080382/149‑1 (MQ=255)                                                           ttCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTccc                                                                                    >  2:329869/1‑146 (MQ=255)                                                           ttCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCAc                                                                                    >  2:329935/1‑148 (MQ=255)                                                           ttCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACt                                                                                   >  1:1362643/1‑149 (MQ=255)                                                            tctggctgATCATCGGAGAAGTTGTTTCGTTAAATAAACACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGAATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACt                                                                                   <  2:268904/148‑1 (MQ=255)                                                                tctgATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTAATGACTATCCCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGc                                                                              >  1:1042517/1‑149 (MQ=255)                                                                 ctgATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGTGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGcc                                                                             <  1:1043830/149‑1 (MQ=255)                                                                 ctgATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGc                                                                              <  2:428849/148‑1 (MQ=255)                                                                   gctcatcGCAGAATTTGTTTCGTTAAATAAAAGCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCcg                                                                            <  2:687206/146‑1 (MQ=255)                                                                           gtgAATTGTTTTCGTTAAATAAATGCTCAGAAGTCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTAt                                                                    <  2:1226027/146‑1 (MQ=255)                                                                           cAGAATTTGGTTCGTTAAATAAAAGCTCAGAAGCCGGTGTCTACGCGGGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTAtt                                                                   <  1:85652/149‑1 (MQ=255)                                                                             gAATTTCTTTCGTTAAATCAAAACTCAGAAGCCGGTGTCTACGCGCGGGTAAAAAGTTCGCTGGGCGGATGTGGGGCATTCATCACTTCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGc                                                                 <  1:1001258/149‑1 (MQ=255)                                                                              aattggctgcGTGAAATAAAACCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGc                                                                 <  2:33915/139‑1 (MQ=255)                                                                                 ttGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAGAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTTCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCtttt                                                             >  1:1232625/1‑147 (MQ=255)                                                                                  tGTTTCGTTAAATAAAAACGCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTAt                                                             <  2:301618/148‑1 (MQ=255)                                                                                   gTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGc                                                           <  1:743909/149‑1 (MQ=255)                                                                                     ttCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTACCAATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTACCGCGCGTTATGGCTTATGCtt                                                         >  1:389996/1‑149 (MQ=255)                                                                                         ttAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTCTCACCGCACGGTTGCCGCGCGTTATTGCTTCTGCTTCGTa                                                     >  1:1269801/1‑149 (MQ=255)                                                                                          taaataaaAACGCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTa                                                     <  2:182138/148‑1 (MQ=255)                                                                                             ataaaAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGc                                                  >  2:454985/1‑148 (MQ=255)                                                                                               aaaaaCTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCGTTTATTACTGCCTATACCTACTGGTTCGTGAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGATTCGTATGCCtt                                               >  1:712807/1‑149 (MQ=255)                                                                                                     tCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCgg                                         >  1:80070/1‑149 (MQ=255)                                                                                                     tCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTACCGCGCGTTATTGCTTCTGCTTCGTTTGCCTTCCTCgg                                         >  1:18155/1‑149 (MQ=255)                                                                                                     tCAGAAGCCGGTGTCCACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCg                                          <  2:761229/148‑1 (MQ=255)                                                                                                       aGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGa                                        <  2:932434/148‑1 (MQ=255)                                                                                                       aGAAGCCGGTCCCCACGCGTGGGTAAAAAGCTCGCCGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGAt                                       <  1:482264/149‑1 (MQ=255)                                                                                                         aaGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCCCTGATAc                                     >  1:606808/1‑149 (MQ=255)                                                                                                                   cTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATAtatta                           <  1:764243/149‑1 (MQ=255)                                                                                                                    tACGCGTTGGTCAAAATTTCGCTGGGCGGAGGTGGGGCATTTAATACGTCCTATTCCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATAtatta                           <  2:20162/148‑1 (MQ=255)                                                                                                                       gcgTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGAGTACGAATTtattctca                        >  2:1164876/1‑144 (MQ=255)                                                                                                                        cgTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACg                      <  1:817084/149‑1 (MQ=255)                                                                                                                               aaaaaGTTCGCTGGGCTGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGtt                <  2:1235559/148‑1 (MQ=255)                                                                                                                               aaaaaGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCCCCTCACTGTTGCCGCGCGTTATTGCTTATGCTCCGTATGCCTTCATCGGCTACGAAGATATTATGACGCCGGtt                >  2:535026/1‑148 (MQ=255)                                                                                                                                 aaaGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTGTACTAGCGGGTTCGTAAACCCGGTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGcc             >  1:870269/1‑149 (MQ=255)                                                                                                                                 aaaGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGc              >  2:455232/1‑148 (MQ=255)                                                                                                                                     ttCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCCCCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGccacca         >  1:865145/1‑149 (MQ=255)                                                                                                                                        gCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGccaccacc       >  2:1132968/1‑148 (MQ=255)                                                                                                                                        gCTCGGCGGAGGTTGCGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCCCTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGccaccacc       <  2:938176/148‑1 (MQ=255)                                                                                                                                            ggCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCTCCACCATTAt  >  1:1127183/1‑149 (MQ=255)                                                                                                                                             gCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCCTATGCCTTCCTCGCATACGAATCTATTATGACGCCGGTTGCCCCCACcatcat  <  2:779909/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               GCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAT  >  CP009273/3219694‑3219981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑