Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,138,811 T→C D338G (GAC→GGC)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,138,8110TC99.1% 351.9 / ‑6.0 108D338G (GAC→GGC) rnefused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (39/67);  total (41/67)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 108 total aligned reads displayed.

TTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATGG  >  CP009273/1138665‑1138955
                                                                                                                                                  |                                                                                                                                                
tttCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCa                                                                                                                                                <  2:1702769/149‑1 (MQ=255)
  tCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCCCGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGCACTGGCGGCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACg                                                                                                                                              >  2:1800836/1‑149 (MQ=255)
    cagcagGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCa                                                                                                                                            >  1:1857885/1‑149 (MQ=255)
     agcagGCCAAAGCGAGAAATATGGCTGATTTGAAGACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAg                                                                                                                                           <  2:1228899/149‑1 (MQ=255)
      gcagGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAgg                                                                                                                                          <  2:1851993/149‑1 (MQ=255)
        agGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGgcg                                                                                                                                        <  2:1737698/149‑1 (MQ=255)
          gCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGGAGTCGATAACAATCGGGCCGCCGAGGCCACGCAGgcgcg                                                                                                                                      >  1:869579/1‑148 (MQ=255)
           ccAAAGCGAGAAATATGGCTGATTTGAATATGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAg                                                                                                                                     <  1:1297052/149‑1 (MQ=255)
            cAAAGCGCGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGc                                                                                                                                    <  1:1515063/149‑1 (MQ=255)
                  gagaAATGTGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCCCCACGAAGACGGTTCTCTACCGCACGCTGGGGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGa                                                                                                                              <  2:201595/149‑1 (MQ=255)
                  gagaAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGa                                                                                                                              <  1:392181/149‑1 (MQ=255)
                   agaAATATGCCTGATTTGAATACGCGAACGGTCCTTACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGGCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAg                                                                                                                             <  2:1722761/149‑1 (MQ=255)
                    gaAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGc                                                                                                                            >  2:806435/1‑149 (MQ=255)
                       atatGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCGGCCGAGGCCACGCAGGCGCAGCTGCCGAGCAAt                                                                                                                         <  1:1467348/149‑1 (MQ=255)
                       atatGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAAt                                                                                                                         <  2:1866555/149‑1 (MQ=255)
                        tatGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAAtc                                                                                                                        >  1:309434/1‑149 (MQ=255)
                            gCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCAt                                                                                                                    >  2:1210956/1‑149 (MQ=255)
                            gCTGATTTGAATACGCGCACGGTCCTGACGAACGCCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCCCGCAGGCGCAGCTGACGAGCACTCTCAt                                                                                                                    >  1:984988/1‑149 (MQ=255)
                             cTGATTTGAATCCGCGCACGCTCCTGACGCACCTCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATc                                                                                                                   <  1:1789066/149‑1 (MQ=255)
                             cTGATTTGAATACGCGCACGGTCCTGTCGCACCGTTTCACGAAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATc                                                                                                                   <  1:267131/149‑1 (MQ=255)
                             cTGACTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATc                                                                                                                   <  1:343016/149‑1 (MQ=255)
                              tGATTTGAATACGCGCACGGTCCGGACGCACCGCTTCATGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCg                                                                                                                  >  1:1081048/1‑149 (MQ=255)
                                aTTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGCGCAATCTCATCGGc                                                                                                                >  1:414151/1‑149 (MQ=255)
                                     aaTACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCtt                                                                                                           >  1:129973/1‑149 (MQ=255)
                                        aCGCACACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGa                                                                                                        <  1:1383680/149‑1 (MQ=255)
                                          gcgcACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAgg                                                                                                      >  2:451099/1‑149 (MQ=255)
                                             cACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTa                                                                                                   <  1:1923396/149‑1 (MQ=255)
                                              accgTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGCGGCGTACTGGCGCCATGTCGATCAAGCCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAg                                                                                                  <  2:1401313/146‑1 (MQ=255)
                                               cggccgtGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGACGTCGATATCACTAATGCCGCCTAGGCCACGCAGGCGCAGCTGACGAGCAATGTCAGCTGCAGCTTCGAGGTTAgt                                                                                                 <  2:394945/143‑1 (MQ=255)
                                               cGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgt                                                                                                 <  2:1634606/149‑1 (MQ=255)
                                               cGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgt                                                                                                 <  2:200154/149‑1 (MQ=255)
                                               cGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgg                                                                                                 <  2:1138246/149‑2 (MQ=255)
                                                tgTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgcg                                                                                                <  2:1378279/148‑3 (MQ=255)
                                                ggTGCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgtg                                                                                                <  1:411489/149‑1 (MQ=255)
                                                 gTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgtgt                                                                                               <  2:538899/149‑1 (MQ=255)
                                                  tCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGCTGAAGTCGACAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCCATCTCATCGGCCGCTTCGAGGTTAGTGtt                                                                                              >  1:1404625/1‑149 (MQ=255)
                                                  tCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCGGCTTCGAGGTTAGTGtt                                                                                              >  2:1225821/1‑149 (MQ=255)
                                                    cTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTaa                                                                                            <  1:942494/149‑1 (MQ=255)
                                                     gggcgcgCCGCTTCACGCGGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTaaa                                                                                           <  2:46256/146‑1 (MQ=255)
                                                         gCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACACTCAGGCCGCCGAGGCCACGCATGCGCAGCTGTCGGGCAATCTCATCGGCAGCTTTGAGGTTAGTGTTAAAcgcg                                                                                       <  2:1305404/149‑1 (MQ=255)
                                                         gCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCGTCGAGGTTAGTGTTAAAcgcg                                                                                       >  1:1872743/1‑149 (MQ=255)
                                                         ccACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAAcgcg                                                                                       <  1:1835165/148‑1 (MQ=255)
                                                         ccACCCCTTCCCGCAGACGGTCGTCTACCGCACGCGGGCGGCGTACCGGCGACACGCCGATGAAGTCGACAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAAcgcg                                                                                       <  2:1123851/148‑1 (MQ=255)
                                                          cACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGCTGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTGGTGTTAAACGCgg                                                                                      >  2:776861/1‑149 (MQ=255)
                                                              gCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttc                                                                                  <  2:52687/149‑1 (MQ=255)
                                                               cTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGGGGTTAGTGTTAAACGCGGTttct                                                                                 >  1:773269/1‑149 (MQ=255)
                                                               cTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttct                                                                                 >  2:1777001/1‑149 (MQ=255)
                                                                ttCACGCCGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttctt                                                                                <  1:300660/149‑1 (MQ=255)
                                                                ttCACGCAGGCGGATTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGACAACAATCAGGCCGCCGAGGCCACGCAGGCGCGGCTGACGAGCAATCTCATCGGCATCTTCTAGGTTAGTGTTAAACGCGGTttctt                                                                                <  2:1454038/149‑1 (MQ=255)
                                                                ttCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGGTTCGAGGTTAGTGTTAAACGCGGTttctt                                                                                <  1:490441/149‑1 (MQ=255)
                                                                tgcACGCGGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGCTGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttctt                                                                                <  2:778087/147‑1 (MQ=255)
                                                                  cACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGGGGTTAGTGTTAAACGCGGTTTCTTCg                                                                              >  1:911498/1‑149 (MQ=255)
                                                                  cACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCg                                                                              >  2:1148574/1‑149 (MQ=255)
                                                                       aGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCAGCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATc                                                                         >  2:874064/1‑149 (MQ=255)
                                                                       aGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGAAAACAGTCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTGAGTGTTAAACGCGGGTTCTTCGATATc                                                                         >  1:281875/1‑149 (MQ=255)
                                                                       aGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTAATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATc                                                                         <  2:1685979/149‑1 (MQ=255)
                                                                       aGACGCTTTTCTACCGCACGCTGGGGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTGCTTCGATATc                                                                         <  1:370164/149‑1 (MQ=255)
                                                                           cgTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATcgccg                                                                     <  1:949427/148‑1 (MQ=255)
                                                                             ttttCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATcgccgcc                                                                   >  2:409471/1‑149 (MQ=255)
                                                                             ttttCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGATGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATACcgccgcc                                                                   >  2:1380509/1‑149 (MQ=255)
                                                                              tttCTGCCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATcgccgccg                                                                  <  2:1479998/149‑1 (MQ=255)
                                                                                 ctcccGCACGGGGGTTGCGTTCTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCCGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCgg                                                               <  2:49067/146‑1 (MQ=255)
                                                                                 cTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCgg                                                               >  1:3603/1‑149 (MQ=255)
                                                                                 cTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCgg                                                               <  1:834587/149‑1 (MQ=255)
                                                                                 cTACCGCACGCTGGTGGCGTACGGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCgg                                                               <  2:27607/149‑1 (MQ=255)
                                                                                 cTACCGCACGCGGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCgg                                                               <  1:1204362/149‑1 (MQ=255)
                                                                                   aCCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGt                                                             <  2:330277/149‑1 (MQ=255)
                                                                                   aCCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCACCTTCGGGGTTAGTGCTCAGCGTGGGTTCTTCGATATCGCCGCCGCGGGt                                                             >  1:271141/1‑149 (MQ=255)
                                                                                    ccGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCCCATCGGCAGCTTCGCGGTTAGTGTTAAACGCGGTCACTTCGATATCGCCGCCGCGGGTc                                                            >  2:60064/1‑149 (MQ=255)
                                                                                            tggtggCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGc                                                    <  1:1657172/149‑1 (MQ=255)
                                                                                             ggtggCGTACTGGCGCCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAAGCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCg                                                   <  1:1936429/149‑1 (MQ=255)
                                                                                               tggCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGa                                                 >  2:321532/1‑149 (MQ=255)
                                                                                                  cGTACTGGCGTCATGTCTATGAAGTCGATAACAACCAGGCCGTCGAGGCCACGCAGGCGCACCTGACGTGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTTAACGCGGTTTCTTCGATATCGCCGCCTCGGGTCGCTCGTGCGGAGtt                                              <  2:152153/149‑1 (MQ=255)
                                                                                                  cGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGtt                                              <  2:1858406/149‑1 (MQ=255)
                                                                                                  cGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGtt                                              <  1:409149/149‑1 (MQ=255)
                                                                                                   gTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTg                                             <  1:1245039/149‑1 (MQ=255)
                                                                                                    tACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTGGCGCGTGCGGAGTTGa                                            >  1:1529960/1‑149 (MQ=255)
                                                                                                     aCTGGCGTCCTGGCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGAt                                           <  2:2918/149‑1 (MQ=255)
                                                                                                      cTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTGCGTGGTTAGCGTCGAATGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGGTGagg                                          >  1:386193/1‑147 (MQ=255)
                                                                                                         gCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCg                                       >  2:114826/1‑149 (MQ=255)
                                                                                                          cGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGCGGCTCGTGCGGAGTTGATGTCGa                                      >  1:1857233/1‑149 (MQ=255)
                                                                                                           ggagtctcgactaaGCCGAAACCACTCAGCGCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGAt                                     <  1:1953380/137‑1 (MQ=255)
                                                                                                            tCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCCCGGGTCGCGCGTGCGGAGTTGaccgctgtg                                    >  1:155236/1‑141 (MQ=255)
                                                                                                                gTCGATGAAGTCGATATCAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACTAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTGTCGGCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGAGGTCGATGGACg                                >  2:1302583/1‑149 (MQ=255)
                                                                                                                   gttGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTa                             <  2:381102/147‑1 (MQ=255)
                                                                                                                   gATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTa                             >  1:1537426/1‑149 (MQ=255)
                                                                                                                    aTGTAGTCGATAACAACCAGGCGGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGTTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTaa                            <  2:892825/149‑1 (MQ=255)
                                                                                                                     tGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCCCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAAc                           <  1:1425164/149‑1 (MQ=255)
                                                                                                                        aGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATAGGCGGCTTCGTGGTTAGTGTTCATCTCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGCTGTTGTGGCTGGCCGTTGACGCt                        >  1:1973099/1‑149 (MQ=255)
                                                                                                                         gTCGATAACACTCAGGCCGCCGAGGCCACGCAGGCGCAGCCGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCtt                       <  1:679966/149‑1 (MQ=255)
                                                                                                                              tAACAATCGGGCTGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTGCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTg                  <  2:1080129/149‑1 (MQ=255)
                                                                                                                                  aaccAGGCCGCCGAGGCCACCCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGt              <  1:853205/146‑1 (MQ=255)
                                                                                                                                  aaTCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGTTGGCCGTTAACCCTTCGGTGCTgc              >  1:541403/1‑148 (MQ=255)
                                                                                                                                    tCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTCAAGGTCGATTGCCGTTAACGCTTCGGTGCTGTCg            <  2:1796801/149‑1 (MQ=255)
                                                                                                                                     cAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGa           <  1:887060/149‑1 (MQ=255)
                                                                                                                                      aGGCCGCCGAGGCCACGCACGCACATCTGGCTAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGAGGTCGATGGCCGTTAACGCTTCGGTGCTGTCGAt          <  2:1291670/149‑1 (MQ=255)
                                                                                                                                            ccgAGGCCACGGAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGGTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAAt    <  2:284446/149‑1 (MQ=255)
                                                                                                                                             cgAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATg   <  2:703902/149‑1 (MQ=255)
                                                                                                                                              gAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATgg  <  1:1809409/149‑1 (MQ=255)
                                                                                                                                              gAGGCCACGCAGGCGCAGCTGACGAGCAACCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATgg  <  1:1611595/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATGG  >  CP009273/1138665‑1138955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑