Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,346,136 G→T R4L (CGC→CTC)  glpA → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,346,1360GT98.0% 300.4 / NA 102R4L (CGC→CTC) glpAsn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (53/47);  minor base C (0/2);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

ATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACG  >  CP009273/2345994‑2346279
                                                                                                                                              |                                                                                                                                               
aTTTACGAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTc                                                                                                                                           <  1:280553/149‑1 (MQ=255)
aTTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTc                                                                                                                                           <  1:590411/149‑1 (MQ=255)
  ttACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTAAGAGGGATAACAATGAAAACTCTCGACTCGc                                                                                                                                         >  2:925426/1‑149 (MQ=255)
    aCAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAACTCAAACAAGTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCaa                                                                                                                                       <  1:78829/149‑1 (MQ=255)
    aCAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCaa                                                                                                                                       <  1:976845/149‑1 (MQ=255)
    aCAAAATGGTGAAAATGCCGCATGAAAGCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGGTAACAATGAAAACTCTCGACGCGCaa                                                                                                                                       <  2:1622675/149‑1 (MQ=255)
     cAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAAt                                                                                                                                      <  1:1193447/149‑1 (MQ=255)
     cAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAAt                                                                                                                                      >  2:1617567/1‑149 (MQ=255)
      aaaaTGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAAAGAACTTCAGAGGGATAACAATGAAAACTCTCAACTCGCAATc                                                                                                                                     >  2:1176283/1‑149 (MQ=255)
       aaaTGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAAtaa                                                                                                                                    >  2:772663/1‑147 (MQ=255)
          tGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGt                                                                                                                                 >  2:102460/1‑149 (MQ=255)
          tGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAAGATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGt                                                                                                                                 <  1:587437/149‑1 (MQ=255)
          tGTTCAAAATGACGCATGAAAACACGTTGCACTTTCGCATTCTGCGCGAATATGCGCGAAATCAAACAATCCATGTTTTTACTATGGCCAAAAGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCCACTCGCAATCAAGt                                                                                                                                 <  2:40766/149‑1 (MQ=255)
            ttCAAAACGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGa                                                                                                                               <  1:877937/149‑1 (MQ=255)
              cAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAAATTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTCACg                                                                                                                             >  2:522487/1‑149 (MQ=255)
              cAAAATGACGCATGAAATCACGGTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACCCGCAATCAAGTGGCg                                                                                                                             >  1:315661/1‑149 (MQ=255)
                aaaTGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTg                                                                                                                           >  2:1358073/1‑149 (MQ=255)
                      cGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGGGACGGGATTATc                                                                                                                     >  1:1456804/1‑149 (MQ=255)
                       gCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCa                                                                                                                    >  2:907846/1‑149 (MQ=255)
                       gCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCa                                                                                                                    <  1:217897/149‑1 (MQ=255)
                         aTGAAATCACGCATCACTTTCGAATTCTGAGCGAATATGCGCGAAATCGAACAATTCATGTTTTAACTATGGCTAAATGGTAAAAAACGAACTGCAGAGGGATAACAATGAAAACTCTCGACTCGCAAGCAAGCGAGGTGATTATCAtt                                                                                                                  >  2:433313/1‑149 (MQ=255)
                           gAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTgg                                                                                                                <  1:937983/149‑1 (MQ=255)
                            aagtCACGTTTCACTTTCGAATTATGAGCGAATAGGAGCGAAATCGAACAATCCCTGTTTTTACTATGGTTAAATGGTAAACAACTAACTTCAGAGAAAAAAAAAAAAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTggc                                                                                                               <  2:1836066/146‑1 (MQ=255)
                            aaaaCACGTTTCACTTTCGAATTATGAGCTAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTggc                                                                                                               <  2:882052/149‑1 (MQ=255)
                            aaaTCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAGTCAAGTGACGTGATTATCATTggc                                                                                                               <  1:1496793/149‑1 (MQ=255)
                            aaaTCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTggc                                                                                                               <  2:817279/149‑1 (MQ=255)
                                    tttCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGgcgc                                                                                                       >  2:23132/1‑149 (MQ=255)
                                       cACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGAAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAAc                                                                                                    >  1:374080/1‑149 (MQ=255)
                                        aCTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACg                                                                                                   >  1:161028/1‑149 (MQ=255)
                                          tttCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTACAAAACGAAGTTCAGAGGGATAACAATGAAACCTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACggg                                                                                                 >  1:928310/1‑149 (MQ=255)
                                          tttCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACggg                                                                                                 >  1:229165/1‑149 (MQ=255)
                                          tttCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACggg                                                                                                 >  2:887685/1‑149 (MQ=255)
                                             cGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGc                                                                                              >  1:1367436/1‑149 (MQ=255)
                                              gAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGcc                                                                                             <  1:412570/149‑1 (MQ=255)
                                               aaTTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCg                                                                                            <  1:1580923/149‑1 (MQ=255)
                                                 ttATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCGACGGGAGCCggg                                                                                          >  2:1400324/1‑149 (MQ=255)
                                                 ttATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCggg                                                                                          >  2:1713940/1‑149 (MQ=255)
                                                 ttATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGACGCAACGGGAGCCggg                                                                                          >  1:779015/1‑149 (MQ=255)
                                                      aGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGCGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTgg                                                                                     >  1:1836040/1‑148 (MQ=255)
                                                       gCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc                                                                                    <  1:1019928/149‑1 (MQ=255)
                                                        ccaaaagacccgAAATTAAACAATTCATGTTTTTACTAGGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATTAAAACTCCCGACTCGCAACCAACTGACGTGATTATCAGTGGCGGCGGCGCAACGGGAGCCGGGATTGccc                                                                                   <  2:919589/139‑1 (MQ=255)
                                                              tGCGCGAAAGCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAATCGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACt                                                                             <  2:1004265/149‑1 (MQ=255)
                                                                 gcgAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGCCGAGGTTATCAGTGGCGGCGCGGCAACGGGAGCCGGGGTTGACCGCGACtgtg                                                                          >  2:1781698/1‑149 (MQ=255)
                                                                  cgAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGc                                                                         <  1:1807994/149‑1 (MQ=255)
                                                                   gAAATCAAACAATTCATGTTTTTACCATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGcc                                                                        <  2:1557311/149‑1 (MQ=255)
                                                                         aaaCACTGCATGTTTTTATTATTGCTAAATTGTAAAAAAGGAACTTCAGAGGGCTACCAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCTTGATTGCTCGCGACTGTGCCCTgcgc                                                                  <  2:406222/149‑1 (MQ=255)
                                                                         aaaCAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGCCTCGCCATCAAGTGACGTGAGTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCGCGCGGCTGTGCCCTgcgc                                                                  >  1:1004748/1‑149 (MQ=255)
                                                                         aaaCAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGGTTGCCCGAGACTGTGCCCTgcgc                                                                  >  1:549959/1‑149 (MQ=255)
                                                                          aaCAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGGCGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGcgcg                                                                 >  1:1360681/1‑149 (MQ=255)
                                                                          aaCAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGcgcg                                                                 <  1:1001138/149‑1 (MQ=255)
                                                                            cAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCGGGGATTGCCCGCGACTGTGCCGTGCGCggg                                                               >  1:54695/1‑149 (MQ=255)
                                                                                  aTGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTgcgc                                                         >  1:240617/1‑149 (MQ=255)
                                                                                  aTGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTgcgc                                                         >  2:1736288/1‑149 (MQ=255)
                                                                                  aTGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCACCGATCGCCCGCGACTGTGCCCTGCGCGGGCTgcgc                                                         <  1:1985549/149‑1 (MQ=255)
                                                                                   tGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGcgcg                                                        >  1:1928626/1‑149 (MQ=255)
                                                                                   tGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGcgcg                                                        >  1:910798/1‑149 (MQ=255)
                                                                                    gtttatgccaccgCTAAACGGACAAAAACGAACCACAGAGGGCTAACAATGAAAACTCTCGACCCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGt                                                       <  1:1953557/137‑1 (MQ=255)
                                                                                    gTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGt                                                       <  2:515434/149‑1 (MQ=255)
                                                                                    gTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGt                                                       <  1:1115591/149‑1 (MQ=255)
                                                                                    gTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGt                                                       <  1:1758759/149‑1 (MQ=255)
                                                                                            tagggCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTgg                                               <  1:486634/146‑1 (MQ=255)
                                                                                            tATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTgg                                               <  1:1097935/149‑1 (MQ=255)
                                                                                            tATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGACTATGTGCTTGCCCTGCGCGGGCTGCGCGTGATTTTgg                                               <  1:1479810/149‑1 (MQ=255)
                                                                                             aTGGGTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCCATGAAGTGACGTGATTATCAGTGGCGGCGGCGCAACGGGGGGCGGGATTGCCGGCGGCTGGGCCCTGCGCGGGCTGCGCGTGATTTTGGt                                              <  2:68955/149‑1 (MQ=255)
                                                                                             aTGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGAGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGt                                              >  2:1688874/1‑149 (MQ=255)
                                                                                             aTGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATAAAGTGACGTGATTATCATTGGCGGAGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTGTGGt                                              >  2:1688805/1‑149 (MQ=255)
                                                                                              tGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGtt                                             <  1:435214/149‑1 (MQ=255)
                                                                                                gCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAGCTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGa                                           <  1:920009/149‑1 (MQ=255)
                                                                                                gCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACCAGCAATCAAGTGAAGTGAATATCATAGGCGGCGTCGCGACGGGAGCCGGTGTTGTCCGCGACTGTGCCCTTCGCGGGCTGCGCGTGATTTTGGTTgg                                           >  1:78418/1‑148 (MQ=255)
                                                                                                 cTAAATGGTAAAAAACGAACTTCATAGGGATAACAATGAAACCTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGTATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAg                                          <  1:814089/149‑1 (MQ=255)
                                                                                                 cTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAg                                          >  2:1631548/1‑149 (MQ=255)
                                                                                                  tAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAgc                                         >  1:1684230/1‑149 (MQ=255)
                                                                                                    aaTGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGGGATTTTGGTTGAgcgc                                       >  2:1352974/1‑149 (MQ=255)
                                                                                                      tGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGGTTTTGGGTGAGCGccc                                     >  2:373628/1‑148 (MQ=255)
                                                                                                       ggTAAAAAACGAACTTCAGAGGGATAACAATGAAAATTCTCGACCCGCATTCAAGTGACGTCATTATCATTGGCGGCGGCGCATCGGGGGTCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCAc                                    <  2:813523/149‑1 (MQ=255)
                                                                                                        gTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACg                                   >  2:153822/1‑149 (MQ=255)
                                                                                                        gTAAAAAACGAACGTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCCCg                                   >  1:1367786/1‑149 (MQ=255)
                                                                                                         tAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGa                                  >  1:536381/1‑149 (MQ=255)
                                                                                                         tAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGACTGCGCGTGATCTTGGTTGAGCGCCACgc                                  >  2:89315/1‑148 (MQ=255)
                                                                                                          aaaaaaCGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGAc                                 <  1:1871169/149‑1 (MQ=255)
                                                                                                             aaaCGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATc                              >  2:1752094/1‑149 (MQ=255)
                                                                                                             aaaCGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATc                              <  1:1636187/149‑1 (MQ=255)
                                                                                                              aaCGAACTTCAGAGGGAAAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCg                             <  2:827216/149‑1 (MQ=255)
                                                                                                               aCGAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGc                            >  1:1494140/1‑149 (MQ=255)
                                                                                                                 gAACTTCAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCaa                          <  2:147534/149‑1 (MQ=255)
                                                                                                                     ttCAGAGGGATAACAATGAAAACCCCCGACTCGCAATCAAGTGACGTTAGTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCgg                      <  1:765311/149‑1 (MQ=255)
                                                                                                                       cAGAGGGATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTg                    <  2:1704981/149‑1 (MQ=255)
                                                                                                                          agGGATAACAATGAAAACTCTCGACTCGAAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCCCGACATCGCAACCGGTGCCa                 >  1:1527421/1‑149 (MQ=255)
                                                                                                                           gggATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGGTTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCAc                >  1:1744863/1‑149 (MQ=255)
                                                                                                                            ggATAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGACTACGCGTGATTTTGGTTGAGCGCCACGACATCTGAACCGGTGCCAcc               >  1:1053400/1‑149 (MQ=255)
                                                                                                                              aTAACAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCgg             <  1:252482/149‑1 (MQ=255)
                                                                                                                                aacaacGAAAACTCTCGACTCGCAATCAAGGGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCATGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGc           <  1:979327/149‑1 (MQ=255)
                                                                                                                                 aCAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCg          >  1:1117341/1‑149 (MQ=255)
                                                                                                                                  cAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGt         <  2:1758045/149‑1 (MQ=255)
                                                                                                                                  cAATGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGAGAGTCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGt         >  1:1337389/1‑149 (MQ=255)
                                                                                                                                   aaTGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGGGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTa        >  1:570708/1‑149 (MQ=255)
                                                                                                                                     tGAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAAc      <  1:26225/149‑1 (MQ=255)
                                                                                                                                      gAAAACTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAAcc     >  1:1618299/1‑149 (MQ=255)
                                                                                                                                        aaaCTCTCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCAc   <  1:1820760/149‑1 (MQ=255)
                                                                                                                                         tcctctCGACTCGCAATCAAGGGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACg  <  1:1653217/147‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
ATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACG  >  CP009273/2345994‑2346279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑