BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,546,084	G→T	H18Q (CAC→CAA)	yjiC ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,546,084	0	G	T	99.2%	427.4 / ‑5.4	128	H18Q (CAC→CAA)	yjiC	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (47/79); total (47/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 142 total aligned reads displayed.

AGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCAT  >  CP009273/4545938‑4546228

 gTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTGTGGTTATTGCACCGGGTAAGAGGCGAAATAGAGCTGTTTTTCTTATCCAGGCGTGCATATTTTAATTTACGTGTAAACTCCCTGTCGACTTTTGGGTGATGAAGGCATTGa                                                                                                                                               <  2:869632/149‑1 (MQ=255)

     tCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTGCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATTGAGGAATTGAGCAt                                                                                                                                           >  1:1325735/1‑149 (MQ=255)

        aaTTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTg                                                                                                                                        <  1:1851072/149‑1 (MQ=255)

         aTTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGTTAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGt                                                                                                                                       <  2:1327268/149‑1 (MQ=255)

          tttCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGGATTGAGCATCTGta                                                                                                                                      >  1:1520316/1‑149 (MQ=255)

          tttCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGta                                                                                                                                      <  1:1002633/149‑1 (MQ=255)

          tttCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGta                                                                                                                                      >  1:92456/1‑149 (MQ=255)

          tttCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGta                                                                                                                                      >  1:1441257/1‑149 (MQ=255)

           ttCGACAACGATTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGGCTATTGGGTGATGAAGGAATTGAGCATCtttca                                                                                                                                     >  1:667406/1‑145 (MQ=255)

            tCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGtaat                                                                                                                                    >  2:256687/1‑149 (MQ=255)

              gactgcccTTTTTCCAGCGTAATTATGGTTATTGCCACGAGTAAGAGGGGGAAGATATATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGtaataa                                                                                                                                  <  2:425260/142‑1 (MQ=255)

                catcggttgttCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATGGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAAtt                                                                                                                                <  2:341599/140‑1 (MQ=255)

                cAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAAtt                                                                                                                                <  1:1936969/149‑1 (MQ=255)

                 aaCGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAAtta                                                                                                                               >  2:901247/1‑148 (MQ=255)

                  aCGGTTTTTCCATAGGAATTATGGTTATTGCCATGAGTAAGAGGCGGAAGATTGATGTTTTACGAATCCATGCGTTCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTg                                                                                                                              <  2:698858/149‑1 (MQ=255)

                   cGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGa                                                                                                                             <  1:239219/149‑1 (MQ=255)

                   cGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGa                                                                                                                             <  2:1742799/149‑1 (MQ=255)

                          tCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGaa                                                                                                                      <  1:1539537/149‑1 (MQ=255)

                           ccctCGTAATTATGGTTATTGCCCCGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAg                                                                                                                     <  2:21083/146‑1 (MQ=255)

                             ctCGTAATTATGGTTAGTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAAACACTTAATATCTGTAATAATTTGACTCTGAAggg                                                                                                                   <  1:490101/148‑1 (MQ=255)

                             aTCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAggg                                                                                                                   >  2:351812/1‑149 (MQ=255)

                               cGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCa                                                                                                                 <  2:254303/149‑1 (MQ=255)

                                gTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCCGAAGGGCAt                                                                                                                >  2:188568/1‑149 (MQ=255)

                                   aTTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGc                                                                                                             >  1:364734/1‑149 (MQ=255)

                                    ttATGGTTATTGCCACGATTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATCTTTTAATTTACGTTTAACCTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCTTCCGTAATAATTTGACTCTGAAGGGCTTTGCt                                                                                                            >  2:1159606/1‑149 (MQ=255)

                                    ttATGGTTATTGCCACGAGTAAGAGGCGGTAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTAGGTGATGAAGGAATTGGGCATCTGTTATAATTTGAGTCTACACGGCATTGCt                                                                                                            >  2:912886/1‑149 (MQ=255)

                                    ttATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCt                                                                                                            >  2:352910/1‑149 (MQ=255)

                                        ggTTATTGCCACGAGTAAGAGGCGGAGGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAAt                                                                                                        <  1:1011331/149‑1 (MQ=255)

                                        ggTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAAt                                                                                                        <  1:477121/149‑1 (MQ=255)

                                        ggTTATTGCCACGAGAAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAAt                                                                                                        <  2:552129/149‑1 (MQ=255)

                                        ggTTATTGCCAAGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCCCTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTGGACTCTGAAGGGCATTGCTTAAt                                                                                                        <  1:252002/149‑1 (MQ=255)

                                         gTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATg                                                                                                       <  1:911284/149‑1 (MQ=255)

                                          ttATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATgg                                                                                                      <  2:1280521/149‑1 (MQ=255)

                                            aTTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCa                                                                                                    <  1:1431735/149‑1 (MQ=255)

                                               gCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAg                                                                                                 >  1:1847874/1‑149 (MQ=255)

                                                ccACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGa                                                                                                >  2:799000/1‑149 (MQ=255)

                                                ccACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTAGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGa                                                                                                >  2:746166/1‑149 (MQ=255)

                                                   cGAGGAAGAGGCAGAAGATAGAGGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACGCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACat                                                                                             <  2:560935/149‑1 (MQ=255)

                                                     aGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataa                                                                                           <  1:1268539/149‑1 (MQ=255)

                                                       tAAGAGGCGGAAGATCGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAATGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                         <  1:1164363/149‑1 (MQ=255)

                                                       tAAGAGGCGGAAGATAGATGTTTTCCGATTCAATGCGTGCGTTTGTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                         <  1:48405/149‑1 (MQ=255)

                                                       tAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                         <  2:1160017/149‑1 (MQ=255)

                                                       tAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                         <  2:201905/149‑1 (MQ=255)

                                                       gaaGAGGCGGAAGATAGATGGTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                         <  1:433910/148‑1 (MQ=255)

                                                         agagGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGt                                                                                       <  2:1250455/149‑1 (MQ=255)

                                                          gagGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCCTAGACATAATAGtt                                                                                      >  1:381783/1‑149 (MQ=255)

                                                           cggCGGAAGATAGATGTTTTCCGAACCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTa                                                                                     <  1:491856/148‑1 (MQ=255)

                                                            ggCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTAc                                                                                    <  1:779517/149‑1 (MQ=255)

                                                             gCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTAcc                                                                                   >  2:479266/1‑149 (MQ=255)

                                                              cGGAAGATAGACGGTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCt                                                                                  <  2:290574/149‑1 (MQ=255)

                                                               ggAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCtt                                                                                 >  2:1129597/1‑149 (MQ=255)

                                                                  agatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTa                                                                              <  1:1662522/149‑1 (MQ=255)

                                                                  agatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTa                                                                              <  1:1732434/149‑1 (MQ=255)

                                                                   gatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTAt                                                                             <  2:1590416/149‑1 (MQ=255)

                                                                   gatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTAt                                                                             <  1:994037/149‑1 (MQ=255)

                                                                    atagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATg                                                                            >  2:1850921/1‑149 (MQ=255)

                                                                           ttttCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTAttt                                                                     <  1:1333280/149‑1 (MQ=255)

                                                                           ttttCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTCTCGACTATTGGGTGATGAAGGAATAGAGCACCTGTAATAATTTGACTCTGAAGGGCATTGCTTAGTGGCATAGACTCAATAGTTACCTTTTATAAATAttt                                                                     >  2:315330/1‑149 (MQ=255)

                                                                             ttCCGAATCCAGTCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCa                                                                   <  2:1510214/149‑1 (MQ=255)

                                                                              tCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCAc                                                                  <  1:382108/149‑1 (MQ=255)

                                                                                 gAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTTGAGGGCATTGATTACTGGCATAGACATAATAGTTACCTTTTATGAGTATTTcccaga                                                               >  2:491266/1‑144 (MQ=255)

                                                                                  aaTCTATGCGTTCATATTTTAATTTACTTTTAAACTCACTGTCGACAATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGAt                                                              <  1:1120125/149‑1 (MQ=255)

                                                                                  aaTCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGAt                                                              >  1:342094/1‑149 (MQ=255)

                                                                                  aaTCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGAt                                                              <  2:1508227/149‑1 (MQ=255)

                                                                                    tCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGt                                                            <  2:1114267/149‑1 (MQ=255)

                                                                                     ccATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTTATGtt                                                           <  2:1130884/149‑1 (MQ=255)

                                                                                       aTGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTa                                                         >  2:924818/1‑149 (MQ=255)

                                                                                              cATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTGATGATTTGACTCTGAAGGGCATTGCTTAATGGAATAGACATACAAGTTACCTTTTATGCGCATTTACCTGATGTTTAGACAAAt                                                  >  2:426427/1‑149 (MQ=255)

                                                                                              cATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGAGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGTCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAAt                                                  <  2:713890/149‑1 (MQ=255)

                                                                                               atatTTTAATTTACGGGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGCCAAATa                                                 <  2:266894/149‑1 (MQ=255)

                                                                                                tatTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAg                                                >  2:1557799/1‑149 (MQ=255)

                                                                                                    ttAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATaa                                            >  2:1415099/1‑149 (MQ=255)

                                                                                                      aaTTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAt                                          <  1:1680762/149‑1 (MQ=255)

                                                                                                          tACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttttt                                      <  2:1344621/149‑1 (MQ=255)

                                                                                                              tgtAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgt                                  <  2:1503696/149‑1 (MQ=255)

                                                                                                               gtAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgta                                 >  1:976963/1‑149 (MQ=255)

                                                                                                               gtAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgta                                 <  1:159698/149‑1 (MQ=255)

                                                                                                                  aaCTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgtagta                              >  2:1453936/1‑149 (MQ=255)

                                                                                                                  aaCTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgtagta                              >  1:710901/1‑149 (MQ=255)

                                                                                                                    cTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTaaa                            <  1:632683/149‑1 (MQ=255)

                                                                                                                       aCTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAag                         <  1:1563984/149‑1 (MQ=255)

                                                                                                                           tatacaaattgtttatgaAGGAATTGAGCATATGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATATTTACCTTTTATGAGTATTTCACTGAAGTAAAAAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGaa                     <  2:1403720/135‑1 (MQ=255)

                                                                                                                           tCGACTATTGGGTGATGAAGGGATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGCTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGaa                     >  1:1422703/1‑149 (MQ=255)

                                                                                                                                tATTGGGTAATGAAGAAATTGCGCATCGGTAATAAATTCACTCTGAAGGGCATTGCATAATGGCATAGACATGATAGTTACATGATATGATTATTTCACTGATGTTGAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTaaa                <  2:132932/149‑1 (MQ=255)

                                                                                                                                  ttGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATCGATAAATTTTTCTGGAGTAAAAAGAGCAGTAAACa              >  1:235022/1‑149 (MQ=255)

                                                                                                                                     ggTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGCTGTCAAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAAt           <  2:920292/149‑1 (MQ=255)

                                                                                                                                       tgatgaAGGAATGGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGa         <  1:879067/149‑1 (MQ=255)

                                                                                                                                        gatgaAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAc        >  2:877553/1‑149 (MQ=255)

                                                                                                                                        gatgaAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAc        >  1:1932875/1‑149 (MQ=255)

                                                                                                                                          tgaAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACTTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGGGAAGTAAACAAATGAaat      >  1:10019/1‑149 (MQ=255)

                                                                                                                                           ggaggaatTGCGCATCTGTAATTGTTTGACGGTGAAGGGCATTTCTTAATGGCATAGACATAATAGGTACCTTTTATGAGTATTGCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatg     <  2:1755169/147‑1 (MQ=255)

                                                                                                                                           gaaggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGGAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatg     >  1:667521/1‑149 (MQ=255)

                                                                                                                                           gaaggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatg     >  1:818963/1‑149 (MQ=255)

                                                                                                                                           gaaggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAAAGAcatg     >  2:1483059/1‑149 (MQ=255)

                                                                                                                                           gaaggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGGAAAAAGAGAAGTAAAAAAATGAcatg     >  2:1745788/1‑149 (MQ=255)

                                                                                                                                           gaaggaagTGAGCATCTGTAATAATTTGACGCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatg     <  1:1266495/149‑1 (MQ=255)

                                                                                                                                            aaggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcattc    >  2:1183138/1‑147 (MQ=255)

                                                                                                                                            aaggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgc    <  2:885519/149‑1 (MQ=255)

                                                                                                                                             aggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgca   >  1:1135142/1‑149 (MQ=255)

                                                                                                                                             aggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAAGGAcatgca   >  2:588156/1‑149 (MQ=255)

AGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCAT  >  CP009273/4545938‑4546228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`