| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 628,974 | C→G | 58.0% | intergenic (‑41/‑68) | ybdH ← / → ybdL | putative oxidoreductase/methionine aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 628,974 | 0 | C | G | 58.0% | 9.0 / 18.3 | 19 | intergenic (‑41/‑68) | ybdH/ybdL | putative oxidoreductase/methionine aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (5/3); new base G (6/5); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTGGCG > CP009273/628834‑629122 | gttgttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGGTAGGAGTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAACCGTCTTGCTTGcc > 1:80629/1‑150 (MQ=255) aaaaaaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTGTTGATTGCCAAATACTAGCATCg > 1:132658/1‑150 (MQ=255) tCGTGCAGGTGATCGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTtat < 1:192405/150‑1 (MQ=255) aGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGcc > 2:246832/1‑150 (MQ=255) aaCTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAAATTTAGGTGT‑TTGCCACCCATT‑CGTAATGTAGACATCTCAACTTCTTGATTGCCGAATCCTAGCATCGTGTTATAGTGCCTTCAACACGc > 2:262962/1‑150 (MQ=255) gCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTCCAATAAAAATGa > 2:24642/1‑150 (MQ=255) ccGACGACCACGCGGATAGGATTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATa < 1:261716/150‑1 (MQ=255) acgacCACGCGGATAGGATTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAAcc < 2:120542/150‑1 (MQ=255) ggATTGTGAGGCAT‑GTTGTGTTGAC‑CTTATTGTTTTATTTAGACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACaaa > 1:218917/1‑150 (MQ=255) ggATTGGGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACaaa < 2:45670/150‑1 (MQ=255) aGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACtt < 2:216040/150‑1 (MQ=255) aT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCcac > 1:153993/1‑150 (MQ=255) gtgttgtC‑CTTATTGTTTTATTTAGACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACtt < 2:118324/150‑1 (MQ=255) gTTTTATTTAGACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCACCACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTAtttt > 2:292525/1‑150 (MQ=255) tatttaGACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCAcc < 2:111440/150‑1 (MQ=255) tatttaGACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCAcc < 2:289422/150‑1 (MQ=255) gACATCTAAACGTGTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGCCACCATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCaagaa > 2:317749/1‑146 (MQ=255) aTCTAAACGTGTTGATTGCCAAATACTAGCATCGTGCTATAGTGCCTTCAACCCACAACTTCGTTAGGTACCATAAAAATGACAAATAACCGTCTGATTCCACAAAGCAAACTTCCACAACTTGGCGCCACTATTTTCACCCAGAAGAgc > 1:299061/1‑150 (MQ=255) tCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTGgcg > 1:11412/1‑150 (MQ=255) | GTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAT‑GTTGTGTTGTC‑CTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTGGCG > CP009273/628834‑629122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |