| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,048,586 | A→T | 43.9% | V18E (GTG→GAG) | glnG ← | fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,048,586 | 0 | A | T | 43.9% | 1.9 / 22.0 | 25 | V18E (GTG→GAG) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base A (9/5); new base T (5/6); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
GCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATC > CP009273/4048446‑4048730 | gcgcCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGCGCGCACGTTCAGGCAcccctcgcc > 1:73965/1‑144 (MQ=255)gcgcCAGCCCGTCCAGTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAGACGTCTGACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGa < 2:302056/150‑1 (MQ=255) cccGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCCCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCg > 2:274547/1‑150 (MQ=255) ccGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGTTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCAACGa > 1:125726/1‑150 (MQ=255) ccGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGTTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGAATGGAACTATCGTCATCGa > 1:146863/1‑150 (MQ=255) atCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCGGTTCTCAAACGTCGGACAGGTTAAACCTCCCCCCGCGACCGCACGTTCAAGCACCCAACGGATGGAACTATCGACATCGACTACCCGCACTAt > 1:4426/1‑150 (MQ=255) cagcaCATCCCGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTg < 2:121495/150‑1 (MQ=255) tGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCGCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTa < 2:66542/150‑1 (MQ=255) cTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTCtt > 1:127492/1‑150 (MQ=255) gcgcCTCCAGCACTTCTGCGCCGTTCCCAAACGTCGTACAGGTTAACCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGAt < 2:73965/150‑1 (MQ=255) cTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGc < 2:205315/150‑1 (MQ=255) cAGCCCTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACTCCCCCCAGCGAGCGCACGTTCCAGCTCCCAACGGATGGAACTATCGCCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTCCCTGATAGGCAgg < 1:176465/150‑1 (MQ=255) cACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTag > 1:227304/1‑149 (MQ=255) cAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATg > 2:51603/1‑150 (MQ=255) cAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGCATGCCCTGGCCAAc > 1:262619/1‑150 (MQ=255) ggTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTg > 2:133704/1‑150 (MQ=255) ggTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGGCATCGACTACCAAGACTAGCCCTCGTTGCATAAACGACACCTTGATTTCGTGATAGGCAGGTAAAGCGAGAACTCGGTTGGCACTAGCCAAccg > 1:321413/1‑148 (MQ=255) aaCCTGCCCCAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaca > 1:292309/1‑148 (MQ=255) aaCCGGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa < 2:212985/150‑1 (MQ=255) ccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAttt > 2:1851/1‑150 (MQ=255) cAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGGAAATTCAAttct > 1:238918/1‑148 (MQ=255) cggccgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGcc < 2:65780/146‑1 (MQ=255) cACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATg < 2:63188/150‑1 (MQ=255) ttCAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGa < 2:356269/150‑1 (MQ=255) cAAGCTCCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGatc < 2:183821/150‑1 (MQ=255) | GCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATC > CP009273/4048446‑4048730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |