| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,419,720 | T→C | 64.7% | D183G (GAT→GGT) | hisJ ← | histidine/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,419,720 | 0 | T | C | 64.7% | 8.8 / 9.2 | 17 | D183G (GAT→GGT) | hisJ | histidine/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base C (4/7); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
TTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCG > CP009273/2419578‑2419865 | ttCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTCGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGaa < 2:238461/150‑1 (MQ=255) ttATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCGTCAATa > 2:49603/1‑150 (MQ=255) gCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAACTTGTTATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATA‑CGTCCGGCAgtcaggtc < 2:84020/150‑1 (MQ=255) ccATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATA‑CGTCCGGCAgtcaggtcag < 2:256456/150‑1 (MQ=255) tttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCCCCGCGATGAGCGACCTCACCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAt < 2:261583/150‑1 (MQ=255) ccACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCtttt > 1:195282/1‑150 (MQ=255) aCCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTTACCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTAcc < 2:206414/150‑1 (MQ=255) aCAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAAc < 2:128145/150‑1 (MQ=255) cAGGTTGTTTGAGGAATCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGTCTTTTGGTGCCCAATGTTCATTACCGAACg < 2:214679/150‑1 (MQ=255) ggTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCCCCCTGGAACGCGGCATCAATA‑CGTCCGCCAGTCAGGTCAGAACAAATGTTGTCCTGCCCCTGATACGCGACGATTTCAATGCCTGTTGAGGCCCAATGTCCATTACCGAACGtc > 1:23919/1‑150 (MQ=255) ggTTGTTTGAGGAAACCTCCGATGCCAGCGACCTCACCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCACAGCTTTTTGGTGCCCAATGTCCACTACCGaagcat > 1:23934/1‑146 (MQ=255) gttgttTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGtct > 1:207420/1‑150 (MQ=255) ttCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTg < 2:283109/150‑1 (MQ=255) tCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCTTTACCGAACGTCTCCTGGGTGGTGCCCTGc < 2:252724/150‑1 (MQ=255) gCTGGCAGCGACCTCACCCTGGAACGCAG‑ATAATGATTTTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGGTCATTACCGACCGTCTCCTGGGTGGTGCCCGGCaa > 1:346072/1‑150 (MQ=255) gCAGCGGCCTCACCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCACTGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACg < 2:105175/150‑1 (MQ=255) cTCATCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGGACGTCTCCTGGCTGGTGCCCTGCAATACGCATCCCCg > 1:173892/1‑150 (MQ=255) | TTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATA‑CGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCG > CP009273/2419578‑2419865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |