| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,353 | A→G | 44.8% | intergenic (‑86/+135) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,353 | 0 | A | G | 44.8% | 3.3 / 30.4 | 29 | intergenic (‑86/+135) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (10/6); new base G (4/9); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01 | |||||||||||
CTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGT > CP009273/2420221‑2420498 | cTGGAGCAGGCCAGCACCAGGTAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTGTTATAGGGAATATTTACAGGTTTGTTACACGTCTCGTTCCATAACATTGCCCATCAAGGCAAGATATCCCGCCCCGTTAg < 1:180646/150‑1 (MQ=255)cTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTGCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg < 1:68176/150‑1 (MQ=255) cAGAACCAGAGAGAGCGATAGCACCAGTTTTTGCATAATGTCCTCAACTGACAGTTTTTTAAAAACGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTAttt < 2:261391/150‑1 (MQ=255) caacAGTTTTTTCATAATGGCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTa < 2:23934/147‑1 (MQ=255) cACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTa < 2:23919/150‑1 (MQ=255) ncAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATg > 2:222824/2‑150 (MQ=255) aTAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGc > 2:235986/1‑150 (MQ=255) aCAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaac < 1:273296/150‑1 (MQ=255) tCTTGTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAc < 2:166375/150‑1 (MQ=255) gggAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑Ca > 2:286340/1‑150 (MQ=255) tttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCatgat < 2:286012/150‑1 (MQ=255) aCAGTTTTTTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATgatgat < 2:251526/150‑1 (MQ=255) aGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCa < 1:249771/150‑1 (MQ=255) ggTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGAATGCATTTCAATGCACCACGATAGAGCAACAAACCTGACGTG‑CACCataccaatcat > 1:162397/1‑141 (MQ=255) ggTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACCAACCTGGCGTG‑CACCATGATGATCAt > 1:131753/1‑150 (MQ=255) ggTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATCTTTCATTAAAAATGCTCAAGTATCCATTTCAATGCACCACGATAGAGCAACAAACCTGGCATC‑CACCATAACGATCAt > 1:120663/1‑150 (MQ=255) ggTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAAGATCCCGCCCCGTTAGACGTATTATTTTTCATGAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCAt < 1:9404/150‑1 (MQ=255) tACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCa > 2:167720/1‑150 (MQ=255) acacTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CAAAatgaaaaggaaaacattag < 1:182078/150‑16 (MQ=255) gTGCCATAAAATTGACCATCAACGCAAGATATCCTGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACACCCCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCtt < 2:62412/150‑1 (MQ=255) ccATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTTccc > 1:136826/1‑150 (MQ=255) gaTAAAATTGACCATCAAGGCAAAGTATCCCGCCCCGTTTGACGTATGATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGGTAGTGCAACAAACCTGGCGAG‑CACCATGACAACCATCCCATCAGGTACACCTTCCCa > 1:268146/2‑150 (MQ=255) cATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTCCCCa > 2:106688/1‑150 (MQ=255) tAAAATTGACCATCAAGGCAGGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTTCCCAGc > 2:260685/1‑150 (MQ=255) aaaTTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGa > 2:276086/1‑150 (MQ=255) aaTTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGAc > 2:138174/1‑150 (MQ=255) cATCAAGGCAAAATTTCCCGCCCCGTTAGACGTATTATTGTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAg < 2:20292/150‑1 (MQ=255) aTCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGGATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGt < 2:206688/150‑1 (MQ=255) gCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATATAAATACAACACGATAGTGCAACAAACCTGGCGTGACAACTTCATG‑TCATTCCTTCAGGGCCTGATTCCCAGCGACGTATCAGTCACCGt > 2:315970/1‑150 (MQ=255) | CTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTG‑CACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGT > CP009273/2420221‑2420498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |